Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.4.1 extracted from

  • Korotchkina, L.G.; Patel, M.S.
    Binding of pyruvate dehydrogenase to the core of the human pyruvate dehydrogenase complex (2008), FEBS Lett., 582, 468-472.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D289A the beta subunit mutant does not have any detectable activity in assays with NAD+ and shows no change in activity in 2,6-dichlorophenolindophenol assays Homo sapiens
D289N the beta subunit mutant shows by 36% reduced activity in assays with NAD+ Homo sapiens
E229A the beta subunit mutant does not show drastic changes compared to the wild type E1 activities Homo sapiens
E229Q the beta subunit mutant does not show drastic changes compared to the wild type E1 activities Homo sapiens
E232A the beta subunit mutant does not show drastic changes compared to the wild type E1 activities Homo sapiens
E232Q the beta subunit mutant does not show drastic changes compared to the wild type E1 activities Homo sapiens
E234A the beta subunit mutant does not show drastic changes compared to the wild type E1 activities Homo sapiens
E234Q the beta subunit mutant does not show drastic changes compared to the wild type E1 activities Homo sapiens
I329A shows 37-43% reduction in activity compared to the wild type enzyme Homo sapiens
additional information mutant I329DELTA (deletion at the C-terminal of E1) shows 62% reduction in activity compared to the wild type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + NAD+
-
Homo sapiens acetyl-CoA + CO2 + NADH
-
?
pyruvate + CoA + oxidized 2,6-dichlorophenolindophenol
-
Homo sapiens acetyl-CoA + CO2 + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
heterotetramer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
pyruvate dehydrogenase E1 bound to the E1-binding domain of dihydrolipoamide acetyltransferase (E2) in pyruvate dehydrogenase complex (PDC) Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens
thiamine diphosphate
-
Homo sapiens