Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D289A | the beta subunit mutant does not have any detectable activity in assays with NAD+ and shows no change in activity in 2,6-dichlorophenolindophenol assays | Homo sapiens |
D289N | the beta subunit mutant shows by 36% reduced activity in assays with NAD+ | Homo sapiens |
E229A | the beta subunit mutant does not show drastic changes compared to the wild type E1 activities | Homo sapiens |
E229Q | the beta subunit mutant does not show drastic changes compared to the wild type E1 activities | Homo sapiens |
E232A | the beta subunit mutant does not show drastic changes compared to the wild type E1 activities | Homo sapiens |
E232Q | the beta subunit mutant does not show drastic changes compared to the wild type E1 activities | Homo sapiens |
E234A | the beta subunit mutant does not show drastic changes compared to the wild type E1 activities | Homo sapiens |
E234Q | the beta subunit mutant does not show drastic changes compared to the wild type E1 activities | Homo sapiens |
I329A | shows 37-43% reduction in activity compared to the wild type enzyme | Homo sapiens |
additional information | mutant I329DELTA (deletion at the C-terminal of E1) shows 62% reduction in activity compared to the wild type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + NAD+ | - |
Homo sapiens | acetyl-CoA + CO2 + NADH | - |
? | |
pyruvate + CoA + oxidized 2,6-dichlorophenolindophenol | - |
Homo sapiens | acetyl-CoA + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
pyruvate dehydrogenase E1 | bound to the E1-binding domain of dihydrolipoamide acetyltransferase (E2) in pyruvate dehydrogenase complex (PDC) | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
thiamine diphosphate | - |
Homo sapiens |