Cloned (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
152000 | - |
- |
Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 | substrate channelling in the multienzyme complex rests on the recognition of the lipoyl domain by enzyme. Cofactor thiamine diphosphate and substrate pyruvate have distinct effects on enzyme/lipoyl domain interaction. conformational freedom is allowed by the linker in the movement of the lipoyl domain between active sites | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | binds to the di-domain (lipoyl domain + linker + peripheral subunit binding domain) of the dihydrolipoyl acetyltransferase (E2) component for acetylation | Geobacillus stearothermophilus | ? | - |
? | |
pyruvate + CoA + NAD+ | - |
Geobacillus stearothermophilus | acetyl-CoA + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | x-ray crystallography | Geobacillus stearothermophilus |
More | substrate channelling in the multienzyme complex rests on the recognition of the lipoyl domain by enzyme. Cofactor thiamine diphosphate and substrate pyruvate have distinct effects on enzyme/lipoyl domain interaction. conformational freedom is allowed by the linker in the movement of the lipoyl domain between active sites | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
E1 component of the pyruvate dehydrogenase multienzyme complex | - |
Geobacillus stearothermophilus |
thiamine diphosphate-dependent 2-oxo acid decarboxylase | - |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent | Geobacillus stearothermophilus |