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Literature summary for 1.2.4.1 extracted from

  • Jeng, J.; Kallarakal, A.T.; Kim, S.F.; Popov, K.M.; Song, B.J.
    Pyruvate dehydrogenase E1alpha isoform in rat testis: cDNA cloning, characterization, and biochemical comparison of the recombinant testis and liver enzymes (1998), Comp. Biochem. Physiol. B, 120, 205-216.
    View publication on PubMed
Search for more literature for 1.2.4.1

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of alpha subunit with human liver beta subunit in Escherichia coli Homo sapiens
co-expression of alpha subunit with human liver beta subunit in Escherichia coli Rattus norvegicus
expression of alpha and beta subunits in Escherichia coli Homo sapiens
expression of alpha and beta subunits in Escherichia coli Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 thiamine diphosphate recombinant enzyme Rattus norvegicus
0.0015
-
 thiamine diphosphate recombinant enzyme Homo sapiens
0.0204
-
 pyruvate recombinant enzyme Rattus norvegicus
0.026
-
 pyruvate recombinant enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.26
-
 recombinant enzyme from testis Rattus norvegicus
8.13
-
 recombinant enzyme from liver Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + benzaldehyde
-
 Homo sapiens (R)-phenylacetylcarbinol
-
 ?
acetaldehyde + benzaldehyde
-
 Rattus norvegicus (R)-phenylacetylcarbinol
-
 ?
pyruvate + CoA + NAD+
-
 Rattus norvegicus acetyl-CoA + CO2 + NADH
-
 ?