BRENDA - Enzyme Database
show all sequences of 1.2.3.6

Influence of the spxB gene on competence in Streptococcus pneumoniae

Baettig, P.; Muehlemann, K.; J. Bacteriol. 190, 1184-1189 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
additional information
pneumococcal spxB gene influences competence, the mechanism remains elusive
Streptococcus pneumoniae D39
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli
Streptococcus pneumoniae D39
Engineering
Protein Variants
Commentary
Organism
additional information
knockout of the spxB gene in strain D39 abolishes spontaneous transformation. Significant reduced expression levels of comC and recA as well as DNA release from bacterial cells during the early growth phase, coincident with the time of spontaneous competence in the parent strain. Supplementation with competencestimulating peptide 1 restores transformation. Neither supplementation with competencestimulating peptide 1 nor supplementation with the SpxB products H2O2 and acetate alteres DNA release. Supplementation of the parent strain with catalase does not reduce DNA release significantly
Streptococcus pneumoniae D39
Organism
Organism
UniProt
Commentary
Textmining
Streptococcus pneumoniae D39
-
serotype 2
-
Synonyms
Synonyms
Commentary
Organism
SpxB
-
Streptococcus pneumoniae D39
Application (protein specific)
Application
Commentary
Organism
additional information
pneumococcal spxB gene influences competence, the mechanism remains elusive
Streptococcus pneumoniae D39
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Streptococcus pneumoniae D39
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
knockout of the spxB gene in strain D39 abolishes spontaneous transformation. Significant reduced expression levels of comC and recA as well as DNA release from bacterial cells during the early growth phase, coincident with the time of spontaneous competence in the parent strain. Supplementation with competencestimulating peptide 1 restores transformation. Neither supplementation with competencestimulating peptide 1 nor supplementation with the SpxB products H2O2 and acetate alteres DNA release. Supplementation of the parent strain with catalase does not reduce DNA release significantly
Streptococcus pneumoniae D39
Other publictions for EC 1.2.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
692839
Baettig
Influence of the spxB gene on ...
Streptococcus pneumoniae D39
J. Bacteriol.
190
1184-1189
2008
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1
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694224
Ramos-Montanez
Polymorphism and regulation of ...
Streptococcus pneumoniae D39
Mol. Microbiol.
67
729-746
2008
1
1
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3
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1
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1
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1
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1
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674287
Regev-Yochay
Interference between Streptoco ...
Streptococcus pneumoniae, Streptococcus pneumoniae PN-20, Streptococcus pneumoniae Rx1
J. Bacteriol.
188
4996-5001
2006
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1
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3
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659039
Seki
Hydrogen peroxide production i ...
no activity in Streptococcus pyogenes
J. Bacteriol.
186
2046-2051
2004
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390438
Takeuchi
Pyruvate oxidase (CoA acetylat ...
Entamoeba histolytica
Biochem. Biophys. Res. Commun.
65
591-596
1975
1
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