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Literature summary for 1.2.3.4 extracted from

  • Chauhan, N.; Hooda, V.; Pundir, C.
    In vitro effects of metal oxide nanoparticles on barley oxalate oxidase (2013), J. Nanopart. Res., 15, 1493.
No PubMed abstract available
Search for more literature for 1.2.3.4

Activating Compound

Activating Compound Comment Organism Structure
diethyldithiocarbamate 35% increase of activity at 1 mM Hordeum vulgare
DW 1750000 21% increase of activity at 1 mM Hordeum vulgare
additional information compared to free enzyme, MnO2 nanoparticles(NP)-bound enzyme shows improved activity (35% stimulation at 2.5 mg/ml) while ZnO NPs- and CuO NPs-bound enzymes have no substantial improvement Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 92% residual activity at 1 mM Hordeum vulgare
K+ 94% residual activity at 1 mM Hordeum vulgare
additional information not influenced by EDTA, NaCl, and MgSO4 Hordeum vulgare
NEM 92% residual activity at 1 mM Hordeum vulgare
Sodium azide 86% residual activity at 1 mM Hordeum vulgare
Sodium molybdate 71% residual activity at 1 mM Hordeum vulgare
Sodium nitrate 45% residual activity at 1 mM Hordeum vulgare
sodium thiocyanate 72% residual activity at 1 mM Hordeum vulgare
ZnSO4 90% residual activity at 1 mM Hordeum vulgare

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 21% increase of activity at 1 mM Hordeum vulgare
Fe2+ 10% increase of activity at 1 mM Hordeum vulgare
Mn2+ 37% increase of activity at 1 mM Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxalate + O2 + H+ Hordeum vulgare
-
 CO2 + H2O2
-
 ?
oxalate + O2 + H+ Hordeum vulgare BH393
-
 CO2 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-
Hordeum vulgare BH393
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel column chromatography and Sephadex G-100 gel filtration Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Hordeum vulgare
-

Storage Stability

Storage Stability Organism
4-8°C, 90 days, native enzyme, up to 70% loss of activity Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxalate + O2 + H+
-
 Hordeum vulgare CO2 + H2O2
-
 ?
oxalate + O2 + H+
-
 Hordeum vulgare BH393 CO2 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
oxalate: O2 oxidoreductase
-
 Hordeum vulgare
OXO
-
 Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 nanoparticle-bound enzyme Hordeum vulgare
40
-
 free enzyme Hordeum vulgare

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 metal oxide nanoparticles(NP)-bound enzyme retains more activity when subjected to thermal treatment at 70°C for 30 min., retention of activity in the increasing order being 54%, 65%, 76%, and 87% for native, ZnO NPs-, CuO NPs-, and MnO2 NPs-bound enzyme, respectively Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2
-
 free enzyme Hordeum vulgare
4
-
 nanoparticle-bound enzyme Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
additional information FMN, FAD, NAD+, and riboflavin at 1 mM in the presence of nanoparticles have practically no effect on native and immobilized enzymes Hordeum vulgare