Crystallization (Comment) | Organism |
---|---|
homology modeling of structure. An N-terminal beta-propeller structure containing the catalytic copper center is linked to a C-terminal immunoglobulin-like domain, with both domains being involved in forming the active site. Residues His804, His889, and Tyr803 compose the first-shell coordination of the copper ion and Cys522-Tyr581 stabilize the free-radical species | Thermothelomyces thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.8 | - |
methylglyoxal | pH 6, 50°C | Thermothelomyces thermophilus | |
20.2 | - |
5-hydroxymethylfurfural | pH 6, 50°C | Thermothelomyces thermophilus | |
471.3 | - |
glycerol | pH 6, 50°C | Thermothelomyces thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | protein contains a radical-coupled copper complex, a mononuclear Cu(II) center with an axial coordination | Thermothelomyces thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces thermophilus | G2Q335 | - |
- |
Thermothelomyces thermophilus DSM 1799 | G2Q335 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxymethylfurfural + H2O + O2 | - |
Thermothelomyces thermophilus | 2,5-diformylfuran + H2O2 | - |
? | |
5-hydroxymethylfurfural + H2O + O2 | - |
Thermothelomyces thermophilus DSM 1799 | 2,5-diformylfuran + H2O2 | - |
? | |
formaldehyde + H2O + O2 | 4% of the activity with 5-hydroxymethylfurfural | Thermothelomyces thermophilus | formate + H2O2 | - |
? | |
formaldehyde + H2O + O2 | 4% of the activity with 5-hydroxymethylfurfural | Thermothelomyces thermophilus DSM 1799 | formate + H2O2 | - |
? | |
glycerol + H2O + O2 | 16% of the activity with 5-hydroxymethylfurfural | Thermothelomyces thermophilus | ? + H2O2 | - |
? | |
glycerol + H2O + O2 | 16% of the activity with 5-hydroxymethylfurfural | Thermothelomyces thermophilus DSM 1799 | ? + H2O2 | - |
? | |
methylglyoxal + H2O + O2 | 96% of the activity with 5-hydroxymethylfurfural | Thermothelomyces thermophilus | methylglyoxylate + H2O2 | - |
? | |
methylglyoxal + H2O + O2 | 96% of the activity with 5-hydroxymethylfurfural | Thermothelomyces thermophilus DSM 1799 | methylglyoxylate + H2O2 | - |
? | |
additional information | no substrates: furfural, 2,5-diformylfuran, glutaraldehyde | Thermothelomyces thermophilus | ? | - |
- |
|
additional information | no substrates: furfural, 2,5-diformylfuran, glutaraldehyde | Thermothelomyces thermophilus DSM 1799 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
GLOX | - |
Thermothelomyces thermophilus |
MYCTH_2294895 | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Thermothelomyces thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
15 min, 50% loss of activity | Thermothelomyces thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.6 | - |
methylglyoxal | pH 6, 50°C | Thermothelomyces thermophilus | |
15.9 | - |
5-hydroxymethylfurfural | pH 6, 50°C | Thermothelomyces thermophilus | |
34.1 | - |
glycerol | pH 6, 50°C | Thermothelomyces thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermothelomyces thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
glycerol | pH 6, 50°C | Thermothelomyces thermophilus | |
0.86 | - |
5-hydroxymethylfurfural | pH 6, 50°C | Thermothelomyces thermophilus | |
0.99 | - |
methylglyoxal | pH 6, 50°C | Thermothelomyces thermophilus |