BRENDA - Enzyme Database show
show all sequences of 1.2.3.15

Glyoxal oxidase of Phanerochaete chrysosporium: its characterization and activation by lignin peroxidase

Kersten, P.J.; Proc. Natl. Acad. Sci. USA 87, 2936-2940 (1990)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.64
-
methylglyoxal
at pH 4.5 and 25°C
Phanerochaete chrysosporium
1.3
-
methylglyoxal
at pH 4.5 and 25°C
Phanerochaete chrysosporium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
full activation takes more than 1 h with 1 mM CuSO4 at pH 6.0
Phanerochaete chrysosporium
additional information
no activation is observed with Mg2+, Zn2+, Fe2+, or Mn2+
Phanerochaete chrysosporium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
1 * 68000, SDS-PAGE
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxopropanal + H2O + O2
Phanerochaete chrysosporium
-
pyruvate + H2O2
-
-
?
2-oxopropanal + H2O + O2
Phanerochaete chrysosporium BKM-F-1767
-
pyruvate + H2O2
-
-
?
glyoxal + H2O + O2
Phanerochaete chrysosporium
-
glyoxylate + H2O2
-
-
?
glyoxal + H2O + O2
Phanerochaete chrysosporium BKM-F-1767
-
glyoxylate + H2O2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phanerochaete chrysosporium
Q01772
-
-
Phanerochaete chrysosporium BKM-F-1767
Q01772
-
-
Purification (Commentary)
Commentary
Organism
DEAE Bio-Gel A column chromatography
Phanerochaete chrysosporium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxopropanal + H2O + O2
-
739561
Phanerochaete chrysosporium
pyruvate + H2O2
-
-
-
?
2-oxopropanal + H2O + O2
-
739561
Phanerochaete chrysosporium BKM-F-1767
pyruvate + H2O2
-
-
-
?
acetaldehyde + H2O + O2
5.7% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
acetaldehyde + H2O + O2
5.7% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium BKM-F-1767
?
-
-
-
?
dihydroxyacetone + H2O + O2
1.6% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
dihydroxyacetone + H2O + O2
1.6% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium BKM-F-1767
?
-
-
-
?
DL-glyceraldehyde + H2O + O2
1.1% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
formaldehyde + H2O + O2
3.6% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
glycolaldehyde + H2O + O2
8.1% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
glyoxal + H2O + O2
-
739561
Phanerochaete chrysosporium
glyoxylate + H2O2
-
-
-
?
glyoxal + H2O + O2
-
739561
Phanerochaete chrysosporium BKM-F-1767
glyoxylate + H2O2
-
-
-
?
glyoxal + H2O + O2
3.3% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
glyoxylic acid + H2O + O2
12.4% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
methylglyoxal + H2O + O2
100% activity
739561
Phanerochaete chrysosporium
methylglyoxylate + H2O2
-
-
-
?
methylglyoxal + H2O + O2
100% activity
739561
Phanerochaete chrysosporium BKM-F-1767
methylglyoxylate + H2O2
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 68000, SDS-PAGE
Phanerochaete chrysosporium
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
198
-
methylglyoxal
at pH 4.5 and 25°C
Phanerochaete chrysosporium
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Phanerochaete chrysosporium
isoelectric focusing
-
4.7
Phanerochaete chrysosporium
isoelectric focusing
-
4.9
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.64
-
methylglyoxal
at pH 4.5 and 25°C
Phanerochaete chrysosporium
1.3
-
methylglyoxal
at pH 4.5 and 25°C
Phanerochaete chrysosporium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
full activation takes more than 1 h with 1 mM CuSO4 at pH 6.0
Phanerochaete chrysosporium
additional information
no activation is observed with Mg2+, Zn2+, Fe2+, or Mn2+
Phanerochaete chrysosporium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
1 * 68000, SDS-PAGE
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxopropanal + H2O + O2
Phanerochaete chrysosporium
-
pyruvate + H2O2
-
-
?
2-oxopropanal + H2O + O2
Phanerochaete chrysosporium BKM-F-1767
-
pyruvate + H2O2
-
-
?
glyoxal + H2O + O2
Phanerochaete chrysosporium
-
glyoxylate + H2O2
-
-
?
glyoxal + H2O + O2
Phanerochaete chrysosporium BKM-F-1767
-
glyoxylate + H2O2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE Bio-Gel A column chromatography
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxopropanal + H2O + O2
-
739561
Phanerochaete chrysosporium
pyruvate + H2O2
-
-
-
?
2-oxopropanal + H2O + O2
-
739561
Phanerochaete chrysosporium BKM-F-1767
pyruvate + H2O2
-
-
-
?
acetaldehyde + H2O + O2
5.7% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
acetaldehyde + H2O + O2
5.7% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium BKM-F-1767
?
-
-
-
?
dihydroxyacetone + H2O + O2
1.6% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
dihydroxyacetone + H2O + O2
1.6% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium BKM-F-1767
?
-
-
-
?
DL-glyceraldehyde + H2O + O2
1.1% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
formaldehyde + H2O + O2
3.6% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
glycolaldehyde + H2O + O2
8.1% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
glyoxal + H2O + O2
-
739561
Phanerochaete chrysosporium
glyoxylate + H2O2
-
-
-
?
glyoxal + H2O + O2
-
739561
Phanerochaete chrysosporium BKM-F-1767
glyoxylate + H2O2
-
-
-
?
glyoxal + H2O + O2
3.3% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
glyoxylic acid + H2O + O2
12.4% activity compared to methylglyoxal
739561
Phanerochaete chrysosporium
?
-
-
-
?
methylglyoxal + H2O + O2
100% activity
739561
Phanerochaete chrysosporium
methylglyoxylate + H2O2
-
-
-
?
methylglyoxal + H2O + O2
100% activity
739561
Phanerochaete chrysosporium BKM-F-1767
methylglyoxylate + H2O2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 68000, SDS-PAGE
Phanerochaete chrysosporium
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
198
-
methylglyoxal
at pH 4.5 and 25°C
Phanerochaete chrysosporium
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Phanerochaete chrysosporium
isoelectric focusing
-
4.7
Phanerochaete chrysosporium
isoelectric focusing
-
4.9
Other publictions for EC 1.2.3.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739130
Son
Heterologous expression of Pha ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
Mycobiology
40
258-262
2012
-
-
1
-
-
-
-
-
-
-
1
2
-
5
-
-
1
-
-
-
-
-
2
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
738564
Whittaker
Glyoxal oxidase from Phaneroch ...
Phanerochaete chrysosporium
J. Biol. Chem.
271
681-687
1996
1
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
1
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738504
Kersten
Phanerochaete chrysosporium gl ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
J. Bacteriol.
177
6106-6110
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
739562
Kersten
Cloning and characterization o ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
Proc. Natl. Acad. Sci. USA
90
7411-7413
1993
-
-
-
-
-
-
-
-
-
-
1
2
-
5
-
1
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
739561
Kersten
Glyoxal oxidase of Phanerochae ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
Proc. Natl. Acad. Sci. USA
87
2936-2940
1990
-
-
-
-
-
-
-
2
-
2
1
4
-
5
-
-
1
-
-
-
-
-
15
1
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
2
1
4
-
-
-
1
-
-
-
-
15
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
738490
Kersten
Involvement of a new enzyme, g ...
Phanerochaete chrysosporium
J. Bacteriol.
169
2195-2201
1987
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
10
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-