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Literature summary for 1.2.3.15 extracted from

  • Kersten, P.J.
    Glyoxal oxidase of Phanerochaete chrysosporium: its characterization and activation by lignin peroxidase (1990), Proc. Natl. Acad. Sci. USA, 87, 2936-2940.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.3.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.64
-
 methylglyoxal at pH 4.5 and 25°C Phanerodontia chrysosporium
1.3
-
 methylglyoxal at pH 4.5 and 25°C Phanerodontia chrysosporium

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ full activation takes more than 1 h with 1 mM CuSO4 at pH 6.0 Phanerodontia chrysosporium
additional information no activation is observed with Mg2+, Zn2+, Fe2+, or Mn2+ Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 1 * 68000, SDS-PAGE Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxopropanal + H2O + O2 Phanerodontia chrysosporium
-
 pyruvate + H2O2
-
 ?
2-oxopropanal + H2O + O2 Phanerodontia chrysosporium BKM-F-1767
-
 pyruvate + H2O2
-
 ?
glyoxal + H2O + O2 Phanerodontia chrysosporium
-
 glyoxylate + H2O2
-
 ?
glyoxal + H2O + O2 Phanerodontia chrysosporium BKM-F-1767
-
 glyoxylate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q01772
-
-
Phanerodontia chrysosporium BKM-F-1767 Q01772
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Bio-Gel A column chromatography Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxopropanal + H2O + O2
-
 Phanerodontia chrysosporium pyruvate + H2O2
-
 ?
2-oxopropanal + H2O + O2
-
 Phanerodontia chrysosporium BKM-F-1767 pyruvate + H2O2
-
 ?
acetaldehyde + H2O + O2 5.7% activity compared to methylglyoxal Phanerodontia chrysosporium ?
-
 ?
acetaldehyde + H2O + O2 5.7% activity compared to methylglyoxal Phanerodontia chrysosporium BKM-F-1767 ?
-
 ?
dihydroxyacetone + H2O + O2 1.6% activity compared to methylglyoxal Phanerodontia chrysosporium ?
-
 ?
dihydroxyacetone + H2O + O2 1.6% activity compared to methylglyoxal Phanerodontia chrysosporium BKM-F-1767 ?
-
 ?
DL-glyceraldehyde + H2O + O2 1.1% activity compared to methylglyoxal Phanerodontia chrysosporium ?
-
 ?
formaldehyde + H2O + O2 3.6% activity compared to methylglyoxal Phanerodontia chrysosporium ?
-
 ?
glycolaldehyde + H2O + O2 8.1% activity compared to methylglyoxal Phanerodontia chrysosporium ?
-
 ?
glyoxal + H2O + O2
-
 Phanerodontia chrysosporium glyoxylate + H2O2
-
 ?
glyoxal + H2O + O2
-
 Phanerodontia chrysosporium BKM-F-1767 glyoxylate + H2O2
-
 ?
glyoxal + H2O + O2 3.3% activity compared to methylglyoxal Phanerodontia chrysosporium ?
-
 ?
glyoxylic acid + H2O + O2 12.4% activity compared to methylglyoxal Phanerodontia chrysosporium ?
-
 ?
methylglyoxal + H2O + O2 100% activity Phanerodontia chrysosporium methylglyoxylate + H2O2
-
 ?
methylglyoxal + H2O + O2 100% activity Phanerodontia chrysosporium BKM-F-1767 methylglyoxylate + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 68000, SDS-PAGE Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
GLOX
-
 Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
198
-
 methylglyoxal at pH 4.5 and 25°C Phanerodontia chrysosporium

pI Value

Organism Comment pI Value Maximum pI Value
Phanerodontia chrysosporium isoelectric focusing
-
 4.7
Phanerodontia chrysosporium isoelectric focusing
-
 4.9