BRENDA - Enzyme Database show
show all sequences of 1.2.3.15

Heterologous expression of Phanerochaete chrysoporium glyoxal oxidase and its application for the coupled reaction with manganese peroxidase to decolorize malachite green

Son, Y.L.; Kim, H.Y.; Thiyagarajan, S.; Xu, J.J.; Park, S.M.; Mycobiology 40, 258-262 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Pichia pastoris strain GS115
Phanerochaete chrysosporium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
x * 70000, SDS-PAGE
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + H2O + O2
Phanerochaete chrysosporium
-
methylglyoxylate + H2O2
-
-
?
methylglyoxal + H2O + O2
Phanerochaete chrysosporium BKM-F-1767
-
methylglyoxylate + H2O2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phanerochaete chrysosporium
Q01772
-
-
Phanerochaete chrysosporium BKM-F-1767
Q01772
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-Histag column chromatography, and gel filtration
Phanerochaete chrysosporium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylglyoxal + H2O + O2
-
739130
Phanerochaete chrysosporium
methylglyoxylate + H2O2
-
-
-
?
methylglyoxal + H2O + O2
-
739130
Phanerochaete chrysosporium BKM-F-1767
methylglyoxylate + H2O2
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 70000, SDS-PAGE
Phanerochaete chrysosporium
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Phanerochaete chrysosporium
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
about 35% activity at 20°C, 100% activity at 30°C, about 95% activity at 40°C, and about 40% activity at 50°C. Less than 20% activity between 60 and 80°C
Phanerochaete chrysosporium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Phanerochaete chrysosporium
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
6
more than 30% activity at pH 4.0 and 6.0, no activity at pH 3.0 and above pH 7.0
Phanerochaete chrysosporium
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pichia pastoris strain GS115
Phanerochaete chrysosporium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
x * 70000, SDS-PAGE
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + H2O + O2
Phanerochaete chrysosporium
-
methylglyoxylate + H2O2
-
-
?
methylglyoxal + H2O + O2
Phanerochaete chrysosporium BKM-F-1767
-
methylglyoxylate + H2O2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-Histag column chromatography, and gel filtration
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylglyoxal + H2O + O2
-
739130
Phanerochaete chrysosporium
methylglyoxylate + H2O2
-
-
-
?
methylglyoxal + H2O + O2
-
739130
Phanerochaete chrysosporium BKM-F-1767
methylglyoxylate + H2O2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 70000, SDS-PAGE
Phanerochaete chrysosporium
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Phanerochaete chrysosporium
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
about 35% activity at 20°C, 100% activity at 30°C, about 95% activity at 40°C, and about 40% activity at 50°C. Less than 20% activity between 60 and 80°C
Phanerochaete chrysosporium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Phanerochaete chrysosporium
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
6
more than 30% activity at pH 4.0 and 6.0, no activity at pH 3.0 and above pH 7.0
Phanerochaete chrysosporium
Other publictions for EC 1.2.3.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739130
Son
Heterologous expression of Pha ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
Mycobiology
40
258-262
2012
-
-
1
-
-
-
-
-
-
-
1
2
-
5
-
-
1
-
-
-
-
-
2
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
738564
Whittaker
Glyoxal oxidase from Phaneroch ...
Phanerochaete chrysosporium
J. Biol. Chem.
271
681-687
1996
1
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
1
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738504
Kersten
Phanerochaete chrysosporium gl ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
J. Bacteriol.
177
6106-6110
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
739562
Kersten
Cloning and characterization o ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
Proc. Natl. Acad. Sci. USA
90
7411-7413
1993
-
-
-
-
-
-
-
-
-
-
1
2
-
5
-
1
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
739561
Kersten
Glyoxal oxidase of Phanerochae ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
Proc. Natl. Acad. Sci. USA
87
2936-2940
1990
-
-
-
-
-
-
-
2
-
2
1
4
-
5
-
-
1
-
-
-
-
-
15
1
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
2
1
4
-
-
-
1
-
-
-
-
15
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
738490
Kersten
Involvement of a new enzyme, g ...
Phanerochaete chrysosporium
J. Bacteriol.
169
2195-2201
1987
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
10
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-