BRENDA - Enzyme Database show
show all sequences of 1.2.3.15

Glyoxal oxidase from Phanerochaete chrysosporium is a new radical-copper oxidase

Whittaker, M.M.; Kersten P.J.; Nakamura, N.; Sanders-Loehr, J.; Schweizer, E.S.; Whittaker, J.W.; J. Biol. Chem. 271, 681-687 (1996)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
purified native glyoxal oxidase is catalytically inactive, but can be oxidatively activated by lignin peroxidase molybdicyanide (K3Mo(CN)8) or hexachloroiridate (Na2IrCl6), or Mn3+ EDTA
Phanerochaete chrysosporium
Cloned(Commentary)
Commentary
Organism
expressed in Aspergillus nidulans
Phanerochaete chrysosporium
Inhibitors
Inhibitors
Commentary
Organism
Structure
phosphate
millimolar inhibitor
Phanerochaete chrysosporium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
0.7-0.8 equivalents of Cu2+ per mol enzyme
Phanerochaete chrysosporium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phanerochaete chrysosporium
Q01772
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylglyoxal + H2O + O2
-
738564
Phanerochaete chrysosporium
methylglyoxylate + H2O2
-
-
-
?
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1
-
phosphate
at pH 6.0, temperature not specified in the publication
Phanerochaete chrysosporium
5.1
-
phosphate
at pH 4.5, temperature not specified in the publication
Phanerochaete chrysosporium
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
purified native glyoxal oxidase is catalytically inactive, but can be oxidatively activated by lignin peroxidase molybdicyanide (K3Mo(CN)8) or hexachloroiridate (Na2IrCl6), or Mn3+ EDTA
Phanerochaete chrysosporium
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Aspergillus nidulans
Phanerochaete chrysosporium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
phosphate
millimolar inhibitor
Phanerochaete chrysosporium
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1
-
phosphate
at pH 6.0, temperature not specified in the publication
Phanerochaete chrysosporium
5.1
-
phosphate
at pH 4.5, temperature not specified in the publication
Phanerochaete chrysosporium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
0.7-0.8 equivalents of Cu2+ per mol enzyme
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylglyoxal + H2O + O2
-
738564
Phanerochaete chrysosporium
methylglyoxylate + H2O2
-
-
-
?
Other publictions for EC 1.2.3.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739130
Son
Heterologous expression of Pha ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
Mycobiology
40
258-262
2012
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-
1
-
-
-
-
-
-
-
1
2
-
5
-
-
1
-
-
-
-
-
2
1
1
1
-
-
1
1
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-
-
-
-
-
-
1
-
-
-
-
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-
-
-
-
-
1
2
-
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-
1
-
-
-
-
2
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
738564
Whittaker
Glyoxal oxidase from Phaneroch ...
Phanerochaete chrysosporium
J. Biol. Chem.
271
681-687
1996
1
-
1
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1
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1
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-
-
1
-
-
-
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-
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1
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2
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1
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1
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1
2
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1
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738504
Kersten
Phanerochaete chrysosporium gl ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
J. Bacteriol.
177
6106-6110
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
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1
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-
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-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
739562
Kersten
Cloning and characterization o ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
Proc. Natl. Acad. Sci. USA
90
7411-7413
1993
-
-
-
-
-
-
-
-
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-
1
2
-
5
-
1
-
-
-
-
-
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2
1
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-
-
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-
-
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1
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1
2
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1
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-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
739561
Kersten
Glyoxal oxidase of Phanerochae ...
Phanerochaete chrysosporium, Phanerochaete chrysosporium BKM-F-1767
Proc. Natl. Acad. Sci. USA
87
2936-2940
1990
-
-
-
-
-
-
-
2
-
2
1
4
-
5
-
-
1
-
-
-
-
-
15
1
-
-
-
1
-
-
-
-
-
2
-
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-
-
-
-
-
-
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2
-
2
1
4
-
-
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1
-
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-
-
15
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
738490
Kersten
Involvement of a new enzyme, g ...
Phanerochaete chrysosporium
J. Bacteriol.
169
2195-2201
1987
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-
-
-
-
-
-
-
-
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1
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-
-
-
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-
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10
-
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1
1
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10
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1
1
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