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Literature summary for 1.2.3.1 extracted from

  • Rashidi, M.R.; Dehghany, M.; Dehghan, G.; Jouyban, A.; Faridi, A.
    Aldehyde oxidase activity and stability in water-miscible organic solvents (2013), Appl. Biochem. Biotechnol., 169, 901-910.
    View publication on PubMed
Search for more literature for 1.2.3.1

Inhibitors

Inhibitors Comment Organism Structure
Pyridine non-linear inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 phenanthridine methanol, pH 7.4, at 35°C Oryctolagus cuniculus
0.00625
-
 phenanthridine pyridine, pH 7.4, at 35°C Oryctolagus cuniculus
0.0063
-
 phenanthridine acetonitrile, pH 7.4, at 35°C Oryctolagus cuniculus
0.00666
-
 phenanthridine dioxane, pH 7.4, at 35°C Oryctolagus cuniculus
0.00769
-
 phenanthridine 2-propanol, pH 7.4, at 35°C Oryctolagus cuniculus
0.00833
-
 phenanthridine 1-propanol, pH 7.4, at 35°C Oryctolagus cuniculus
0.00833
-
 phenanthridine ethanol, pH 7.4, at 35°C Oryctolagus cuniculus
0.01
-
 phenanthridine N-N-dimethylformamide, pH 7.4, at 35°C Oryctolagus cuniculus
0.011
-
 phenanthridine phosphate buffer, pH 7.4, at 35°C Oryctolagus cuniculus
0.011
-
 phenanthridine tetrahydrofuran, pH 7.4, at 35°C Oryctolagus cuniculus

Organic Solvent Stability

Organic Solvent Comment Organism
Methanol concentration of half inactivation = 0.5% Oryctolagus cuniculus
additional information in the case of all organic solvents, the activity of aldehyde oxidase decreased when the concentration of the solvent increased in the reaction medium Oryctolagus cuniculus
N,N-dimethylformamide concentration of half inactivation = 13.3% Oryctolagus cuniculus
Pyridine concentration of half inactivation = 0.5% Oryctolagus cuniculus
tetrahydrofuran concentration of half inactivation = 0.5% Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus P80456 New Zealand rabbit
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by heat treatment and ammonium sulfate precipitation Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 in the case of all organic solvents, the activity of aldehyde oxidase decreases when the concentration of the solvent increases in the reaction medium Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenanthridine + H2O + O2 activity of rabbit liver aldehyde oxidase characterized in nine water-miscible organic mixtures i.e. phosphate buffer mixed with N-N-dimethylformamide, acetonitrile, tetrahydrofuran, 1-propanol, 2-propanol, ethanol, pyridine, dioxane, and methanol Oryctolagus cuniculus ?
-
 ?

Synonyms

Synonyms Comment Organism
AOX1
-
 Oryctolagus cuniculus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 reduction in the enzyme activity at all temperatures in the presence of all organic solvents, except methanol and acetonitrile, enzyme activity in the presence of these two solvents increases with a rise in temperature up to 45°C followed by a marked reduction at 55°C, behavior of aldehyde oxidase is similar to that in the organic solvent free buffer solution Oryctolagus cuniculus
25 55 in the organic solvent-free buffer, a significant increase in the aldehyde oxidase activity observable as temperature raises up to 45°C while enzyme activity decreases almost to zero when the reaction temperature is elevated to 55°C Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 incubation of aldehyde oxidase in all water-miscible organic solvents results in a marked loss of the catalytic activity, residual activity of aldehyde oxidase in the presence of dioxane, ethanol, N,N-dimethylformamide, and pyridine is 16%, 10%, 12%, and 17% after 24 h at 35°C Oryctolagus cuniculus
35
-
 the activity remains almost unchanged after 24 h in the absence of organic solvents at 35°C, enzyme is relatively stable in the presence of methanol, 2-propanol and acetonitrile Oryctolagus cuniculus
55
-
 at 55°C, the enzyme undergoes a conformational change producing an inactive form, conversion is reversible Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 the manner of the enzyme activity in the hydro-organic mixtures at different pHs is almost similar to that observed in the phosphate buffer system Oryctolagus cuniculus
9
-
 phosphate buffer with no added organic solvent shows the highest aldehyde oxidase activity Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 aldehyde oxidase active in either the organic solvent-free buffer or all nine water-miscible organic solvents, lowest enzyme activity is obtained at pH 6.0 with acetonitrile Oryctolagus cuniculus