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Literature summary for 1.2.3.1 extracted from

  • Itoh, K.; Asakawa, T.; Hoshino, K.; Adachi, M.; Fukiya, K.; Watanabe, N.; Tanaka, Y.
    Functional analysis of aldehyde oxidase using expressed chimeric enzyme between monkey and rat (2009), Biol. Pharm. Bull., 32, 31-35.
    View publication on PubMed
Search for more literature for 1.2.3.1

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeric cDNAs by mutual exchange of 2Fe-2S/FAD and MoCo domains between cynomolgous monkey and rat. Chimeric monkey/rat AO is referred to one with monkey type 2Fe-2S/FAD domains and a rat type MoCo domain. Rat/monkey AO is vice versa. Substrate inhibition is seen in rat AO and chimeric monkey/rat AO, but not in monkey AO and chimeric rat/monkey AO. A biphasic Eadie-Hofstee profile is observed in monkey AO and chimeric rat/monkey AO, but not rat AO and chimeric monkey/rat AO. Two-fold greater Vmax values are observed in monkey AO than in chimeric rat/monkey AO, and in chimeric monkey/rat AO than in rat AO Rattus norvegicus
additional information construction of chimeric cDNAs by mutual exchange of 2Fe-2S/FAD and MoCo domains between cynomolgous monkey and rat. Chimeric monkey/rat AO is referred to one with monkey type 2Fe-2S/FAD domains and a rat type MoCo domain. Rat/monkey AO is vice versa. Substrate inhibition is seen in rat AO and chimeric monkey/rat AO, but not in monkey AO and chimeric rat/monkey AO. A biphasic EadieHofstee profile is observed in monkey AO and chimeric rat/monkey AO, but not rat AO and chimeric monkey/rat AO. Two-fold greater Vmax values are observed in monkey AO than in chimeric rat/monkey AO, and in chimeric monkey/rat AO than in rat AO Macaca fascicularis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043
-
 4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine chimeric rat/monkey enzyme, high affinity value, pH 6.0, 37°C Rattus norvegicus
0.0043
-
 4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine chimeric rat/monkey enzyme, high affinity value, pH 6.0, 37°C Macaca fascicularis
0.0164
-
 4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine wild-type, low affinity value, pH 6.0, 37°C Macaca fascicularis
0.0171
-
 4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine chimeric rat/monkey enzyme, low affinity value, pH 6.0, 37°C Rattus norvegicus
0.0171
-
 4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine chimeric rat/monkey enzyme, low affinity value, pH 6.0, 37°C Macaca fascicularis
0.0351
-
 4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine wild-type, high affinity value, pH 6.0, 37°C Macaca fascicularis
0.0363
-
 4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine chimeric monkey/rat enzyme, pH 6.0, 37°C Rattus norvegicus
0.0363
-
 4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine chimeric monkey/rat enzyme, pH 6.0, 37°C Macaca fascicularis
0.0528
-
 4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine wild-type, pH 6.0, 37°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Macaca fascicularis
-
-
-
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine + H2O + O2 i.e. RS-8359, 2-oxidation of the S-enantiomer Rattus norvegicus ? + H2O2
-
 ?
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine + H2O + O2 i.e. RS-8359, 2-oxidation of the S-enantiomer Macaca fascicularis ? + H2O2
-
 ?