BRENDA - Enzyme Database
show all sequences of 1.2.2.B1

Membrane-associated quinoprotein formaldehyde dehydrogenase from Methylococcus capsulatus bath

Zahn, J.A.; Bergmann, D.J.; Boyd, J.M.; Kunz, R.C.; DiSpirito, A.A.; J. Bacteriol. 183, 6832-6840 (2001)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
partial inhibition
Methylococcus capsulatus
Fe2+
partial inhibition
Methylococcus capsulatus
additional information
the enzyme is insensitive to EDTA, N-ethylmaleimide, cyanide and azide
Methylococcus capsulatus
phenylhydrazine
-
Methylococcus capsulatus
Zn2+
partial inhibition
Methylococcus capsulatus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.143
-
formaldehyde
20°C, pH 7.4
Methylococcus capsulatus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
associated
Methylococcus capsulatus
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49500
-
4 * 49500, SDS-PAGE
Methylococcus capsulatus
210000
-
gel filtration
Methylococcus capsulatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
formaldehyde + ferricytochrome b559/569 complex
Methylococcus capsulatus
the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium
formate + ferrocytochrome b559/569 complex
-
-
?
formaldehyde + ferricytochrome b559/569 complex
Methylococcus capsulatus Bath
the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium
formate + ferrocytochrome b559/569 complex
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Methylococcus capsulatus
-
-
-
Methylococcus capsulatus Bath
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Methylococcus capsulatus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:high copper medium-grown cell
-
Methylococcus capsulatus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
146
-
-
Methylococcus capsulatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
formaldehyde + dichloroindophenol
formaldehyde is the only substrate that supports enzyme-mediated reduction of dichloroindophenol
288469
Methylococcus capsulatus
formate + reduced dichloroindophenol
-
-
-
?
formaldehyde + dichloroindophenol
formaldehyde is the only substrate that supports enzyme-mediated reduction of dichloroindophenol
288469
Methylococcus capsulatus Bath
formate + reduced dichloroindophenol
-
-
-
?
formaldehyde + ferricytochrome b559/569 + H2O
-
288469
Methylococcus capsulatus
formate + ferrocytochrome b559/569 + 2 H+
-
-
-
r
formaldehyde + ferricytochrome b559/569 + H2O
-
288469
Methylococcus capsulatus Bath
formate + ferrocytochrome b559/569 + 2 H+
-
-
-
r
formaldehyde + ferricytochrome b559/569 complex
-
288469
Methylococcus capsulatus
formate + ferrocytochrome b559/569 complex
-
-
-
?
formaldehyde + ferricytochrome b559/569 complex
the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium
288469
Methylococcus capsulatus
formate + ferrocytochrome b559/569 complex
-
-
-
?
formaldehyde + ferricytochrome b559/569 complex
-
288469
Methylococcus capsulatus Bath
formate + ferrocytochrome b559/569 complex
-
-
-
?
formaldehyde + ferricytochrome b559/569 complex
the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium
288469
Methylococcus capsulatus Bath
formate + ferrocytochrome b559/569 complex
-
-
-
?
additional information
no activity with: methanol, acetaldehyde, glyoxal, or butyraldehyde
288469
Methylococcus capsulatus
?
-
-
-
?
additional information
no activity with: methanol, acetaldehyde, glyoxal, or butyraldehyde
288469
Methylococcus capsulatus Bath
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 49500, SDS-PAGE
Methylococcus capsulatus
Synonyms
Synonyms
Commentary
Organism
DL-FalDH
-
Methylococcus capsulatus
formaldehyde-cytochrome b559/569 oxidoreductase
-
Methylococcus capsulatus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
266
-
ferricytochrome b559/569
20°C, pH 7.4
Methylococcus capsulatus
290
-
dichloroindophenol
20°C, pH 7.4
Methylococcus capsulatus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Methylococcus capsulatus
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
pyrroloquinoline quinone-to-subunit stochiometry of approximately 1:1, 3.6 mol of pyrroloquinoline quinone per mol of enzyme
Methylococcus capsulatus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
pyrroloquinoline quinone-to-subunit stochiometry of approximately 1:1, 3.6 mol of pyrroloquinoline quinone per mol of enzyme
Methylococcus capsulatus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
partial inhibition
Methylococcus capsulatus
Fe2+
partial inhibition
Methylococcus capsulatus
additional information
the enzyme is insensitive to EDTA, N-ethylmaleimide, cyanide and azide
Methylococcus capsulatus
phenylhydrazine
-
Methylococcus capsulatus
Zn2+
partial inhibition
Methylococcus capsulatus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.143
-
formaldehyde
20°C, pH 7.4
Methylococcus capsulatus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
associated
Methylococcus capsulatus
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49500
-
4 * 49500, SDS-PAGE
Methylococcus capsulatus
210000
-
gel filtration
Methylococcus capsulatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
formaldehyde + ferricytochrome b559/569 complex
Methylococcus capsulatus
the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium
formate + ferrocytochrome b559/569 complex
-
-
?
formaldehyde + ferricytochrome b559/569 complex
Methylococcus capsulatus Bath
the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium
formate + ferrocytochrome b559/569 complex
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methylococcus capsulatus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:high copper medium-grown cell
-
Methylococcus capsulatus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
146
-
-
Methylococcus capsulatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
formaldehyde + dichloroindophenol
formaldehyde is the only substrate that supports enzyme-mediated reduction of dichloroindophenol
288469
Methylococcus capsulatus
formate + reduced dichloroindophenol
-
-
-
?
formaldehyde + dichloroindophenol
formaldehyde is the only substrate that supports enzyme-mediated reduction of dichloroindophenol
288469
Methylococcus capsulatus Bath
formate + reduced dichloroindophenol
-
-
-
?
formaldehyde + ferricytochrome b559/569 + H2O
-
288469
Methylococcus capsulatus
formate + ferrocytochrome b559/569 + 2 H+
-
-
-
r
formaldehyde + ferricytochrome b559/569 + H2O
-
288469
Methylococcus capsulatus Bath
formate + ferrocytochrome b559/569 + 2 H+
-
-
-
r
formaldehyde + ferricytochrome b559/569 complex
-
288469
Methylococcus capsulatus
formate + ferrocytochrome b559/569 complex
-
-
-
?
formaldehyde + ferricytochrome b559/569 complex
the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium
288469
Methylococcus capsulatus
formate + ferrocytochrome b559/569 complex
-
-
-
?
formaldehyde + ferricytochrome b559/569 complex
-
288469
Methylococcus capsulatus Bath
formate + ferrocytochrome b559/569 complex
-
-
-
?
formaldehyde + ferricytochrome b559/569 complex
the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium
288469
Methylococcus capsulatus Bath
formate + ferrocytochrome b559/569 complex
-
-
-
?
additional information
no activity with: methanol, acetaldehyde, glyoxal, or butyraldehyde
288469
Methylococcus capsulatus
?
-
-
-
?
additional information
no activity with: methanol, acetaldehyde, glyoxal, or butyraldehyde
288469
Methylococcus capsulatus Bath
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 49500, SDS-PAGE
Methylococcus capsulatus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
266
-
ferricytochrome b559/569
20°C, pH 7.4
Methylococcus capsulatus
290
-
dichloroindophenol
20°C, pH 7.4
Methylococcus capsulatus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Methylococcus capsulatus
Other publictions for EC 1.2.2.B1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
288469
Zahn
Membrane-associated quinoprote ...
Methylococcus capsulatus, Methylococcus capsulatus Bath
J. Bacteriol.
183
6832-6840
2001
-
-
-
-
-
-
5
1
1
-
2
2
-
44
-
-
1
-
-
1
1
-
10
1
2
-
-
-
2
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
1
1
-
2
2
-
-
-
1
-
1
1
-
10
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-