KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value of NADH is below 0.5 mM, of NADPH is above 1 mM | Clostridium autoethanogenum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | enzyme complex comprises 6% of the cytoplasmic proteins | Clostridium autoethanogenum | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium autoethanogenum | S5Z061 and S5YUC5 | S5Z061: [FeFe]-hydrogenase subunit HytA, S5YUC5: formate dehydrogenase subunit FdhA | - |
Clostridium autoethanogenum DSM 10061 | S5Z061 and S5YUC5 | S5Z061: [FeFe]-hydrogenase subunit HytA, S5YUC5: formate dehydrogenase subunit FdhA | - |
Oxidation Stability | Organism |
---|---|
the enzyme is rapidly inactivated in the presence of only trace amounts of O2, and it loses activity upon dilution to much below 1 mg/ml even under strictly anoxic conditions | Clostridium autoethanogenum |
Purification (Comment) | Organism |
---|---|
- |
Clostridium autoethanogenum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7 | - |
formate formation reaction, pH 7.5, 37°C | Clostridium autoethanogenum |
8.7 | - |
H2 formation reaction, pH 7.5, 37°C | Clostridium autoethanogenum |
13 | - |
substrate H2, pH 6.5, 37°C | Clostridium autoethanogenum |
15.2 | - |
substrate formate, pH 7.5, 37°C | Clostridium autoethanogenum |
26.5 | - |
H2 formation reaction, pH 6.0, 37°C | Clostridium autoethanogenum |
29.2 | - |
substrate H2, pH 7.5, 37°C | Clostridium autoethanogenum |
32 | - |
substrate H2, pH 6.5, 37°C | Clostridium autoethanogenum |
35 | - |
formate formation, substrates CO2 and H2, pH 7.5, 37°C | Clostridium autoethanogenum |
41 | - |
formate formation, substrates CO2 and H2, pH 7.0, 37°C | Clostridium autoethanogenum |
18000 | - |
substrates H2 and methyl viologen, pH 7.5, 37°C | Clostridium autoethanogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 CO2 + NADPH + H+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
Clostridium autoethanogenum | 2 formate + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
r | |
2 CO2 + NADPH + H+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
Clostridium autoethanogenum DSM 10061 | 2 formate + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
r | |
2 formate + NAD+ + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
Clostridium autoethanogenum | 2 CO2 + NADH + H+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
r | |
2 formate + NAD+ + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
Clostridium autoethanogenum DSM 10061 | 2 CO2 + NADH + H+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
r | |
2 formate + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
Clostridium autoethanogenum | 2 CO2 + NADPH + H+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
r | |
2 formate + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
Clostridium autoethanogenum DSM 10061 | 2 CO2 + NADPH + H+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
r | |
H2 + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
Clostridium autoethanogenum | NADPH + 3 H+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
r | |
H2 + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
Clostridium autoethanogenum DSM 10061 | NADPH + 3 H+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
r | |
H2 + NADP+ + 2 oxidized methyl viologen | - |
Clostridium autoethanogenum | ? + NADPH + H+ + 2 reduced methyl viologen | - |
r | |
H2 + NADP+ + 2 oxidized methyl viologen | - |
Clostridium autoethanogenum DSM 10061 | ? + NADPH + H+ + 2 reduced methyl viologen | - |
r | |
NADPH + 3 H+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
Clostridium autoethanogenum | H2 + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | purified complex is composed of seven different subunits. The 78.8-kDa subunit FdhA is a selenocysteine- and tungsten-containing formate dehydrogenase, the 65.5-kDa subunit HytB is an ironsulfur flavin mononucleotide protein harboring the NADP binding site, the 51.4-kDa subunit HytA is the [FeFe]-hydrogenase, and the 18.1-kDa HytC, 28.6-kDa HytD, 19.9-kDa HytE1, and 20.1-kDa HytE2 subunits are iron-sulfur proteins | Clostridium autoethanogenum |
Synonyms | Comment | Organism |
---|---|---|
electron-bifurcating [FeFe]-hydrogenase | - |
Clostridium autoethanogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium autoethanogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
H2 formation from formate | Clostridium autoethanogenum |
6 | - |
H2 formation from NADPH and reduced ferredoxin | Clostridium autoethanogenum |
6.5 | - |
NADP and ferredoxin reduction with H2 | Clostridium autoethanogenum |
7 | - |
CO2 reduction with H2 to formate | Clostridium autoethanogenum |
7.5 | - |
NADP and ferredoxin reduction with formate at pH 7.5 | Clostridium autoethanogenum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | Km value is above 1 mM, NADPH is preferred over NADH | Clostridium autoethanogenum | |
NADPH | Km value is below 0.5 mM, NADPH is preferred over NADH | Clostridium autoethanogenum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme complex catalyzes both the reversible coupled reduction of ferredoxin and NADP+ with H2 or formate and the reversible formation of H2 and CO2 from formate. The complex has two functions in vivo, namely, to normally catalyze CO2 reduction to formate with NADPH and reduced ferredoxin in the Wood-Ljungdahl pathway and to catalyze H2 formation from NADPH and reduced ferredoxin when these redox mediators get too reduced during unbalanced growth on CO | Clostridium autoethanogenum |