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Literature summary for 1.2.1.99 extracted from

  • Ashok, S.; Sankaranarayanan, M.; Ko, Y.; Jae, K.E.; Ainala, S.K.; Kumar, V.; Park, S.
    Production of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae DELTAdhaTDELTAyqhD which can produce vitamin B12 naturally (2013), Biotechnol. Bioeng., 110, 511-524.
    View publication on PubMed
Search for more literature for 1.2.1.99

Application

Application Comment Organism
synthesis 3-hydroxypropionic acid is an important platform chemical that can be used to synthesize a range of chemical compounds, such as acrylic acid, 1,3-propanediol, methyl acrylate, malonic acid, acrylamide, and hydroxyamides Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
PuuC of Klebsiella pneumoniae alone or with coenzyme B12-dependent glycerol dehydratase, DhaB overexpressed homologously, and two major oxidoreductases, DhaT and YqhD, disrupted resulting in KpC_DELTA_dhaT_DELTA_yqhD strain and KpBC_DELTA_dhaT_DELTA_yqhD strain, overexpressing the glycerol dehydratase DhaB, too Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 PuuC protein in recombinant Klebsiella pneumoniae, SDS–PAGE Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity varies depending on the type of strain and culture conditions, e.g. aeration conditions and working volume Klebsiella pneumoniae
1.5
-
 recombinant KpBC_DELTA_dhaT_DELTA_yqhD strain, pH 8.0, 45°C Klebsiella pneumoniae
2.5
-
 recombinant KpC_DELTA_dhaT_DELTA_yqhD strain, pH 8.0, 45°C Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropionaldehyde + NAD+ + H2O 3-hydroxypropionaldehyde i.e. 3-HPA, 3-hydroxypropionic acid i.e. 3-HP, PuuC overexpression enhances the glycerol carbon flux towards 3-hydroxypropionaldehyde Klebsiella pneumoniae 3-hydroxypropanoate + NADH + 2 H+
-
 ?
additional information development and evaluation recombinant Klebsiella pneumoniae, for the production of 3-hydroxypropionic acid from glycerol, therefor PuuC together with a coenzyme B12-dependent glycerol dehydratase are overexpressed while two major oxidoreductases, DhaT and YqhD, are disrupted eliminating the production of 1,3-propanediol out of 3-hydroxypropionaldehyde Klebsiella pneumoniae ?
-
 ?

Synonyms

Synonyms Comment Organism
NAD+-dependent gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase
-
 Klebsiella pneumoniae
PuuC
-
 Klebsiella pneumoniae