Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.99 extracted from

  • Jo, J.E.; Mohan Raj, S.; Rathnasingh, C.; Selvakumar, E.; Jung, W.C.; Park, S.
    Cloning, expression, and characterization of an aldehyde dehydrogenase from Escherichia coli K-12 that utilizes 3-hydroxypropionaldehyde as a substrate (2008), Appl. Microbiol. Biotechnol., 81, 51-60.
    View publication on PubMed
Search for more literature for 1.2.1.99

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, activity increases by 27% Escherichia coli
dithiothreitol 1 mM, activity increases by 75% Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
cloned into Escherichia coli BL21 under the T5 promoter using the pQE-80L vector Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, complete inhibition Escherichia coli
Co2+ 1 mM, 49.7% inhibition Escherichia coli
Cu2+ 1 mM, 97.1% inhibition Escherichia coli
EDTA 1 mM, 14.7% inhibition Escherichia coli
Fe2+ 1 mM, 42.5% inhibition Escherichia coli
Hg2+ 1 mM, complete inhibition Escherichia coli
Li+ 1 mM, 97% inhibition Escherichia coli
Mg2+ 1 mM, 19.4% inhibition Escherichia coli
Mn2+ 1 mM, 15.2% inhibition Escherichia coli
NaCl 1 mM, 16.4% inhibition Escherichia coli
NH4Cl 1 mM, 21% inhibition Escherichia coli
Ni2+ 1 mM, 27.6% inhibition Escherichia coli
Semicarbazide hydrochloride 1 mM, 13.3% inhibition Escherichia coli
Sodium bisulfite 1 mM, 98% inhibition Escherichia coli
Zn2+ 1 mM, 93% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 3-hydroxypropionate pH 8.0, 37°C, cofactor: NADH Escherichia coli
0.24
-
 Valeraldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
0.29
-
 3-hydroxypropionaldehyde pH 8.0, 37°C, cofactor: NADP+ Escherichia coli
0.49
-
 3-hydroxypropionaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
0.68
-
 Isovaleraldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
0.97
-
 Butyraldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
1
-
 acetaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
1.21
-
 propionaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
5.37
-
 benzaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropionaldehyde + NAD+ + H2O Escherichia coli
-
 3-hydroxypropanoate + NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P23883
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 Vmax: 3-hydroxypropionate, pH 8.0, 37°C, cofactor: NADH Escherichia coli
5.5
-
 Vmax: 3-hydroxypropionaldehyde, pH 8.0, 37°C, cofactor: NADP+ Escherichia coli
12.39
-
 Vmax: acetaldehyde, pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
19.51
-
 Vmax: benzaldehyde, pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
27.35
-
 Vmax: propionaldehyde, pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
29.47
-
 Vmax: valeraldehyde, pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
30.07
-
 Vmax: butyraldehyde, pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
30.67
-
 Vmax: isovaleraldehyde, pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
32.1
-
 Vmax: 3-hydroxypropionaldehyde, pH 8.0, 37°C, cofactor: NAD+ Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropionaldehyde + NAD+ + H2O
-
 Escherichia coli 3-hydroxypropanoate + NADH + 2 H+
-
 ?
3-hydroxypropionaldehyde + NAD+ + H2O activity for the reduction of 3-hydroxypropionate to 3-hydroxypropionaldehyde is only 4% of that for the corresponding oxidation reaction, indicating that this enzyme catalyzes oxidation reactions under most physiological conditions Escherichia coli 3-hydroxypropanoate + NADH + 2 H+
-
 r
3-hydroxypropionaldehyde + NADP+ + H2O activity is decreased by 73% when NAD+ is replaced with NADP+. The reduction of 3-hydroxypropionate to 3-hydroxypropionaldehyde is not catalyzed with NADPH as cofactor Escherichia coli 3-hydroxypropanoate + NADPH + 2 H+
-
 ir
acetaldehyde + NAD+ + H2O
-
 Escherichia coli acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Escherichia coli benzoate + NADH + H+
-
 ?
butyraldehyde + NAD+ + H2O
-
 Escherichia coli butyrate + NADH + 2 H+
-
 ?
isovaleraldehyde + NAD+ + H2O
-
 Escherichia coli 3-methylbutanoate + NADH + 2 H+
-
 ?
propionaldehyde + NAD+ + H2O
-
 Escherichia coli propionate + NADH + 2 H+
-
 ?
valeraldehyde + NAD+ + H2O most preferred substrate Escherichia coli pentanoate + NADH + H+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 35% of maximal activity, 50°C: about 65% of maximal activity Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
 3-hydroxypropionate pH 8.0, 37°C, cofactor: NADH Escherichia coli
4.91
-
 3-hydroxypropionaldehyde pH 8.0, 37°C, cofactor: NADP+ Escherichia coli
11.03
-
 acetaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
17.37
-
 benzaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
24.35
-
 propionaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
26.24
-
 Valeraldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
26.95
-
 Butyraldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
27.31
-
 Isovaleraldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
28.54
-
 3-hydroxypropionaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 25% of maximal activity, pH 9.0: about 20% of maximal activity Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+ utilizes both NAD+ and NADP+ as cofactors, NAD+ is the preferred cofactor for all substrates except benzaldehyde and 2-furaldehyde. In case of 3-hydroxypropionaldehyde, the activity is decreased by 73% when NAD+ is replaced with NADP+ Escherichia coli
NADP+ utilizes both NAD+ and NADP+ as cofactors, NAD+ is the preferred cofactor for all substrates except benzaldehyde and 2-furaldehyde. In case of 3-hydroxypropionaldehyde, the activity is decreased by 73% when NAD+ is replaced with NADP+ Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2
-
 3-hydroxypropionate pH 8.0, 37°C, cofactor: NADH Escherichia coli
3.23
-
 benzaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
11.03
-
 acetaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
16.92
-
 3-hydroxypropionaldehyde pH 8.0, 37°C, cofactor: NADP+ Escherichia coli
20.12
-
 propionaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
27.79
-
 Butyraldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
40.16
-
 Isovaleraldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
58.57
-
 3-hydroxypropionaldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli
109
-
 Valeraldehyde pH 8.0, 37°C, cofactor: NAD+ Escherichia coli