BRENDA - Enzyme Database show
show all sequences of 1.2.1.96

Separation and partial characterization of the enzymes of the toluene-4-monooxygenase catabolic pathway in Pseudomonas mendocina KR1

Whited, G.M.; Gibson, D.T.; J. Bacteriol. 173, 3017-3020 (1991)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxybenzaldehyde + NADP+ + H2O
Pseudomonas mendocina
involved in the degradation pathway of toluene
4-hydroxybenzoate + NADPH + H+
-
Pseudomonas mendocina
?
4-hydroxybenzaldehyde + NADP+ + H2O
Pseudomonas mendocina KR1
involved in the degradation pathway of toluene
4-hydroxybenzoate + NADPH + H+
-
Pseudomonas mendocina KR1
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas mendocina
-
-
-
Pseudomonas mendocina KR1
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Pseudomonas mendocina
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas mendocina
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.17
-
-
Pseudomonas mendocina
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxybenzaldehyde + NADP+ + H2O
involved in the degradation pathway of toluene
288314
Pseudomonas mendocina
4-hydroxybenzoate + NADPH + H+
-
288314
Pseudomonas mendocina
?
4-hydroxybenzaldehyde + NADP+ + H2O
involved in the degradation pathway of toluene
288314
Pseudomonas mendocina KR1
4-hydroxybenzoate + NADPH + H+
-
288314
Pseudomonas mendocina KR1
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Pseudomonas mendocina
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Pseudomonas mendocina
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxybenzaldehyde + NADP+ + H2O
Pseudomonas mendocina
involved in the degradation pathway of toluene
4-hydroxybenzoate + NADPH + H+
-
Pseudomonas mendocina
?
4-hydroxybenzaldehyde + NADP+ + H2O
Pseudomonas mendocina KR1
involved in the degradation pathway of toluene
4-hydroxybenzoate + NADPH + H+
-
Pseudomonas mendocina KR1
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Pseudomonas mendocina
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas mendocina
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.17
-
-
Pseudomonas mendocina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxybenzaldehyde + NADP+ + H2O
involved in the degradation pathway of toluene
288314
Pseudomonas mendocina
4-hydroxybenzoate + NADPH + H+
-
288314
Pseudomonas mendocina
?
4-hydroxybenzaldehyde + NADP+ + H2O
involved in the degradation pathway of toluene
288314
Pseudomonas mendocina KR1
4-hydroxybenzoate + NADPH + H+
-
288314
Pseudomonas mendocina KR1
?
Other publictions for EC 1.2.1.96
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741220
Mukherjee
Redirection of metabolite bios ...
Daucus carota
Planta
243
305-320
2016
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1
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1
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726788
Chen
-
The catabolism of 2,4-xylenol ...
Pseudomonas putida, Pseudomonas putida NCIMB 9866
Appl. Microbiol. Biotechnol.
98
1349-1356
2014
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1
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4
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2
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2
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1
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8
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4
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1
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1
1
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4
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2
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1
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8
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4
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4
4
288312
Cronin
Organization and sequences of ...
Pseudomonas putida
DNA Seq.
10
7-17
1999
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1
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1
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1
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1
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733948
Miller Jr
-
Bioconversion of toluene to p- ...
Pseudomonas putida, Pseudomonas putida EM2839
Green Chem.
1
143-152
1999
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2
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2
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2
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1
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1
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2
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288314
Whited
Separation and partial charact ...
Pseudomonas mendocina, Pseudomonas mendocina KR1
J. Bacteriol.
173
3017-3020
1991
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2
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2
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1
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1
1
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2
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1
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1
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2
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1
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1
1
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