BRENDA - Enzyme Database
show all sequences of 1.2.1.95

Domain structure characterization of the multifunctional alpha-aminoadipate reductase from Penicillium chrysogenum by limited proteolysis. Activation of alpha-aminoadipate does not require the peptidyl carrier protein box or the reduction domain

Hijarrubia, M.J.; Aparicio, J.F.; Martin, J.F.; J. Biol. Chem. 278, 8250-8256 (2003)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
5 mM, 75% inhibition
Penicillium chrysogenum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
NADPH
pH 9.0, 30°C
Penicillium chrysogenum
1.3
-
ATP
pH 9.0, 30°C
Penicillium chrysogenum
1.4
-
L-2-aminoadipate
pH 9.0, 30°C
Penicillium chrysogenum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolutely required for activity, optimal concentration 10 mM
Penicillium chrysogenum
Mn2+
can partly replace Mg2+, leading to 60% of maximum activity with Mg2+
Penicillium chrysogenum
additional information
no activity with Ca2+, Co2+, Hg2+, Cu2+
Penicillium chrysogenum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
154800
-
1 * 155000, SDS-PAGE, 1 * 154800, calculated
Penicillium chrysogenum
155000
-
1 * 155000, SDS-PAGE, 1 * 154800, calculated
Penicillium chrysogenum
171000
-
gel filtration
Penicillium chrysogenum
Organism
Organism
UniProt
Commentary
Textmining
Penicillium chrysogenum
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Penicillium chrysogenum
Storage Stability
Storage Stability
Organism
-20°C, stable
Penicillium chrysogenum
4°C, stable
Penicillium chrysogenum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-2-aminoadipate + NADPH + H+ + ATP
-
656194
Penicillium chrysogenum
(S)-2-amino-6-oxohexanoate + NADP+ + AMP + diphosphate
-
-
-
?
additional information
no substrates: adipic acid, DL-diaminopimelic acid, L-glutamate
656194
Penicillium chrysogenum
?
-
-
-
-
S-carboxymethyl-L-cysteine + NADPH + H+ + ATP
20fold lower activity than with L-2-aminoadipate
656194
Penicillium chrysogenum
? + NADP+ + AMP + diphosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 155000, SDS-PAGE, 1 * 154800, calculated
Penicillium chrysogenum
Synonyms
Synonyms
Commentary
Organism
alpha-AAR
-
Penicillium chrysogenum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
29
-
Penicillium chrysogenum
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
-
rapid loss of activity above
Penicillium chrysogenum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
1h, 20% loss of activity
Penicillium chrysogenum
37
-
15 min, 50% loss of activity
Penicillium chrysogenum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1
-
L-2-aminoadipate
pH 9.0, 30°C
Penicillium chrysogenum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Penicillium chrysogenum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9.5
-
Penicillium chrysogenum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
5 mM, 75% inhibition
Penicillium chrysogenum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
NADPH
pH 9.0, 30°C
Penicillium chrysogenum
1.3
-
ATP
pH 9.0, 30°C
Penicillium chrysogenum
1.4
-
L-2-aminoadipate
pH 9.0, 30°C
Penicillium chrysogenum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolutely required for activity, optimal concentration 10 mM
Penicillium chrysogenum
Mn2+
can partly replace Mg2+, leading to 60% of maximum activity with Mg2+
Penicillium chrysogenum
additional information
no activity with Ca2+, Co2+, Hg2+, Cu2+
Penicillium chrysogenum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
154800
-
1 * 155000, SDS-PAGE, 1 * 154800, calculated
Penicillium chrysogenum
155000
-
1 * 155000, SDS-PAGE, 1 * 154800, calculated
Penicillium chrysogenum
171000
-
gel filtration
Penicillium chrysogenum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Penicillium chrysogenum
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable
Penicillium chrysogenum
4°C, stable
Penicillium chrysogenum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-2-aminoadipate + NADPH + H+ + ATP
-
656194
Penicillium chrysogenum
(S)-2-amino-6-oxohexanoate + NADP+ + AMP + diphosphate
-
-
-
?
additional information
no substrates: adipic acid, DL-diaminopimelic acid, L-glutamate
656194
Penicillium chrysogenum
?
-
-
-
-
S-carboxymethyl-L-cysteine + NADPH + H+ + ATP
20fold lower activity than with L-2-aminoadipate
656194
Penicillium chrysogenum
? + NADP+ + AMP + diphosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 155000, SDS-PAGE, 1 * 154800, calculated
Penicillium chrysogenum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
29
-
Penicillium chrysogenum
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
-
rapid loss of activity above
Penicillium chrysogenum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
1h, 20% loss of activity
Penicillium chrysogenum
37
-
15 min, 50% loss of activity
Penicillium chrysogenum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1
-
L-2-aminoadipate
pH 9.0, 30°C
Penicillium chrysogenum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Penicillium chrysogenum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9.5
-
Penicillium chrysogenum
Other publictions for EC 1.2.1.95
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733659
Kalb
Activity of alpha-aminoadipate ...
Gelatoporia subvermispora, Gelatoporia subvermispora B
ChemBioChem
16
1426-1430
2015
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742278
Kalb
Activity of alpha-aminoadipat ...
Gelatoporia subvermispora
ChemBioChem
16
1426-1430
2015
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
1
1
4
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
695274
Yan
Cloning, sequencing and charac ...
Saccharomycopsis fibuligera, Saccharomycopsis fibuligera PD70
Yeast
24
189-199
2007
1
1
1
-
-
-
1
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657420
Guo
Posttranslational activation, ...
Schizosaccharomyces pombe
Yeast
21
1279-1288
2004
-
-
1
-
4
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656194
Hijarrubia
Domain structure characterizat ...
Penicillium chrysogenum
J. Biol. Chem.
278
8250-8256
2003
-
-
-
-
-
-
1
3
-
3
3
-
-
2
-
-
1
-
-
-
-
2
3
1
1
1
1
2
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
3
3
-
-
-
-
1
-
-
-
2
3
1
1
1
2
1
1
1
-
-
-
-
-
-
-
-
656800
Guo
Site-directed mutational analy ...
Candida albicans
Mol. Genet. Genomics
269
271-279
2003
-
-
1
-
61
-
-
-
-
1
-
-
-
5
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
61
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288203
Hijarrubia
Characterization of the lys2 g ...
Acremonium chrysogenum, Acremonium chrysogenum ATCC 48272
Mol. Gen. Genet.
264
755-762
2001
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288204
Guo
Novel posttranslational activa ...
Candida albicans
J. Bacteriol.
183
7120-7125
2001
-
-
1
-
7
-
-
-
-
-
1
-
-
4
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288201
Ehmann
Lysine biosynthesis in Sacchar ...
Saccharomyces cerevisiae
Biochemistry
38
6171-6177
1999
-
-
1
-
-
-
-
5
-
-
1
-
-
3
-
1
-
-
-
-
-
-
4
1
1
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
-
-
-
-
4
1
-
-
-
4
-
-
-
-
-
-
-
-
4
4
288198
Ford
Molecular properties of the ly ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe 972
Curr. Genet.
28
131-137
1995
-
-
1
-
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
733601
Lu
Regulation of alpha-aminoadipa ...
Penicillium chrysogenum
Can. J. Microbiol.
38
758-763
1992
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733888
Affenzeller
-
Lysine biosynthesis in Penicil ...
Penicillium chrysogenum
FEMS Microbiol. Lett.
58
293-297
1989
-
-
-
-
-
-
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-