Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermoplasma acidophilum |
Crystallization (Comment) | Organism |
---|---|
wild type enzyme, sitting drop vapor diffusion method, using 50 mM MOPS, 50 mM sodium HEPES pH 7.5, 20% (w/v) ethylene glycol, 10% (w/v) PEG 8000, 20 mM glutamic acid (racemic), 20 mM glycine, 20 mM serine (racemic), 20 mM alanine (racemic), 20 mM lysine-HCl (racemic). Mutant enzyme F34M/S405N, sitting drop vapor diffusion method, using 50 mM MES, 50 mM imidazole pH 6.5, 8.3% (w/v) PEG 8000, 16.7% (w/v) ethylene glycol, 20 mM sodium formate, 20 mM sodium citrate, 20 mM sodium oxamate, 20 mM ammonium acetate, 20 mM sodium potassium tartrate | Thermoplasma acidophilum |
Protein Variants | Comment | Organism |
---|---|---|
F34M/S405N | the mutant shows a slight increase in NAD+ acceptance and a 9fold improvement in catalytic efficiency with NAD+ compared with the wild type enzyme | Thermoplasma acidophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde + NADP+ + H2O | Thermoplasma acidophilum | - |
D-glycerate + NADPH + H+ | - |
? | |
D-glyceraldehyde + NADP+ + H2O | Thermoplasma acidophilum ATCC 25905 | - |
D-glycerate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | Q9HK01 | - |
- |
Thermoplasma acidophilum ATCC 25905 | Q9HK01 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde + NADP+ + H2O | - |
Thermoplasma acidophilum | D-glycerate + NADPH + H+ | - |
? | |
D-glyceraldehyde + NADP+ + H2O | - |
Thermoplasma acidophilum ATCC 25905 | D-glycerate + NADPH + H+ | - |
? | |
additional information | NAD+ is a poor substrate | Thermoplasma acidophilum | ? | - |
- |
|
additional information | NAD+ is a poor substrate | Thermoplasma acidophilum ATCC 25905 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 56446, calculated from amino acid sequence | Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
ALDH | - |
Thermoplasma acidophilum |
glyceraldehyde dehydrogenase | - |
Thermoplasma acidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermoplasma acidophilum |