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Literature summary for 1.2.1.88 extracted from

  • Mantilla, B.S.; Paes, L.S.; Pral, E.M.; Martil, D.E.; Thiemann, O.H.; Fernandez-Silva, P.; Bastos, E.L.; Silber, A.M.
    Role of delta1-pyrroline-5-carboxylate dehydrogenase supports mitochondrial metabolism and host-cell invasion of Trypanosoma cruzi (2015), J. Biol. Chem., 290, 7767-7790.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
NADP+ pH 7.2, 28°C Trypanosoma cruzi
97
-
L-glutamate 5-semialdehyde pH 7.2, 28°C Trypanosoma cruzi
103
-
NAD+ pH 7.2, 28°C Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Trypanosoma cruzi 16020
-
mitochondrion
-
Trypanosoma cruzi 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62400
-
-
Trypanosoma cruzi
64000
-
-
Trypanosoma cruzi
364600
-
SAXS distance distribution function calculation Trypanosoma cruzi
649300
-
dynamic light scattering Trypanosoma cruzi
714900
-
gel filtration Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q4DRT8
-
-
Trypanosoma cruzi CL Brener Q4DRT8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD+ + H2O
-
Trypanosoma cruzi L-glutamate + NADH + H+
-
?
1-pyrroline-5-carboxylate + NAD+ + H2O
-
Trypanosoma cruzi CL Brener L-glutamate + NADH + H+
-
?
L-glutamate 5-semialdehyde + NADP+ + H2O
-
Trypanosoma cruzi L-glutamate + NADPH + H+
-
?
L-glutamate 5-semialdehyde + NADP+ + H2O
-
Trypanosoma cruzi CL Brener L-glutamate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
multimer x * 62400, calculated, x * 64000, SDS-PAGE Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
P5CDH1
-
Trypanosoma cruzi
Tc00.1047053510943.50
-
Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
NADP+ pH 7.2, 28°C Trypanosoma cruzi
7
-
L-glutamate 5-semialdehyde pH 7.2, 28°C Trypanosoma cruzi
7.5
-
NAD+ pH 7.2, 28°C Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Trypanosoma cruzi

Expression

Organism Comment Expression
Trypanosoma cruzi 6fold upregulation during the infective stages of the parasite up

General Information

General Information Comment Organism
physiological function gene is able to complement a yeast strain deficient in PUT2 activity Trypanosoma cruzi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.072
-
L-glutamate 5-semialdehyde pH 7.2, 28°C Trypanosoma cruzi
0.0723
-
NAD+ pH 7.2, 28°C Trypanosoma cruzi
0.342
-
NADP+ pH 7.2, 28°C Trypanosoma cruzi