Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
NADP+ | pH 7.2, 28°C | Trypanosoma cruzi | |
97 | - |
L-glutamate 5-semialdehyde | pH 7.2, 28°C | Trypanosoma cruzi | |
103 | - |
NAD+ | pH 7.2, 28°C | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Trypanosoma cruzi | 16020 | - |
mitochondrion | - |
Trypanosoma cruzi | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62400 | - |
- |
Trypanosoma cruzi |
64000 | - |
- |
Trypanosoma cruzi |
364600 | - |
SAXS distance distribution function calculation | Trypanosoma cruzi |
649300 | - |
dynamic light scattering | Trypanosoma cruzi |
714900 | - |
gel filtration | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q4DRT8 | - |
- |
Trypanosoma cruzi CL Brener | Q4DRT8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NAD+ + H2O | - |
Trypanosoma cruzi | L-glutamate + NADH + H+ | - |
? | |
1-pyrroline-5-carboxylate + NAD+ + H2O | - |
Trypanosoma cruzi CL Brener | L-glutamate + NADH + H+ | - |
? | |
L-glutamate 5-semialdehyde + NADP+ + H2O | - |
Trypanosoma cruzi | L-glutamate + NADPH + H+ | - |
? | |
L-glutamate 5-semialdehyde + NADP+ + H2O | - |
Trypanosoma cruzi CL Brener | L-glutamate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 62400, calculated, x * 64000, SDS-PAGE | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
P5CDH1 | - |
Trypanosoma cruzi |
Tc00.1047053510943.50 | - |
Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
NADP+ | pH 7.2, 28°C | Trypanosoma cruzi | |
7 | - |
L-glutamate 5-semialdehyde | pH 7.2, 28°C | Trypanosoma cruzi | |
7.5 | - |
NAD+ | pH 7.2, 28°C | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Trypanosoma cruzi |
Organism | Comment | Expression |
---|---|---|
Trypanosoma cruzi | 6fold upregulation during the infective stages of the parasite | up |
General Information | Comment | Organism |
---|---|---|
physiological function | gene is able to complement a yeast strain deficient in PUT2 activity | Trypanosoma cruzi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
L-glutamate 5-semialdehyde | pH 7.2, 28°C | Trypanosoma cruzi | |
0.0723 | - |
NAD+ | pH 7.2, 28°C | Trypanosoma cruzi | |
0.342 | - |
NADP+ | pH 7.2, 28°C | Trypanosoma cruzi |