BRENDA - Enzyme Database
show all sequences of 1.2.1.87

Oxygen-tolerant coenzyme A-acylating aldehyde dehydrogenase facilitates efficient photosynthetic n-butanol biosynthesis in cyanobacteria

Lan, E.; Ro, S.; Liao, J.; Energy Environ. Sci. 6, 2672-2681 (2013)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Klebsiella pneumoniae produces more n-butanol than ethanol
Klebsiella pneumoniae
gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Listeria monocytogenes produces more ethanol than n-butanol
Listeria monocytogenes
gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Salmonella enterica produces more ethanol than n-butanol
Salmonella enterica
Engineering
Protein Variants
Commentary
Organism
additional information
design of a coenzyme A (CoA) dependent n-butanol biosynthesis pathway tailored to the metabolic physiology of the cyanobacterium Synechococcus elongatus PCC 7942 by incorporating an ATP driving force and a kinetically irreversible trap. Oxygen-sensitive CoA-acylating butyraldehyde dehydrogenase (Bldh) is exchanged for the oxygen-tolerant PduP from Salmonella enterica. Replacing Bldh with PduP in the n-butanol synthesis pathway results in n-butanol production to a cumulative titer of 404 mg/l with peak productivity of 51 mg/l per day, exceeding the base strain by 20fold. Anaerobic growth rescue of Escherichia coli strain JCL166 by overexpression of the Clostridium butanol pathway with different aldehyde dehydrogenases PduP
Salmonella enterica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kientics
Listeria monocytogenes
0.056
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
0.0717
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
0.087
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
0.0926
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
0.181
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
0.342
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
acetyl-CoA + NADH + H+
Klebsiella pneumoniae
-
acetaldehyde + CoA + NAD+
-
-
r
acetyl-CoA + NADH + H+
Salmonella enterica
-
acetaldehyde + CoA + NAD+
-
-
r
acetyl-CoA + NADH + H+
Listeria monocytogenes
low activity
acetaldehyde + CoA + NAD+
-
-
r
acetyl-CoA + NADH + H+
Listeria monocytogenes M7
low activity
acetaldehyde + CoA + NAD+
-
-
r
butanoyl-CoA + NADH + H+
Salmonella enterica
-
butanal + CoA + NAD+
-
-
r
butanoyl-CoA + NADH + H+
Listeria monocytogenes
-
butanal + CoA + NAD+
-
-
r
butanoyl-CoA + NADH + H+
Klebsiella pneumoniae
high activity
butanal + CoA + NAD+
-
-
r
butanoyl-CoA + NADH + H+
Listeria monocytogenes M7
-
butanal + CoA + NAD+
-
-
r
propanoyl-CoA + NADH + H+
Klebsiella pneumoniae
best substrate
propanal + CoA + NAD+
-
-
r
propanoyl-CoA + NADH + H+
Salmonella enterica
best substrate
propanal + CoA + NAD+
-
-
r
propanoyl-CoA + NADH + H+
Listeria monocytogenes
best substrate
propanal + CoA + NAD+
-
-
r
propanoyl-CoA + NADH + H+
Listeria monocytogenes M7
best substrate
propanal + CoA + NAD+
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Klebsiella pneumoniae
-
-
-
Listeria monocytogenes
A0A0E0UUX4
-
-
Listeria monocytogenes M7
A0A0E0UUX4
-
-
Salmonella enterica
-
-
-
Oxidation Stability
Oxidation Stability
Organism
the enzyme is oxygen-tolerant
Klebsiella pneumoniae
the enzyme is oxygen-tolerant
Salmonella enterica
the enzyme is oxygen-tolerant
Listeria monocytogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.9
-
with butanoyl-CoA, pH 7.15, 30°C
Klebsiella pneumoniae
17
-
with butanoyl-CoA, pH 7.15, 30°C
Listeria monocytogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetyl-CoA + NADH + H+
-
742372
Klebsiella pneumoniae
acetaldehyde + CoA + NAD+
-
-
-
r
acetyl-CoA + NADH + H+
-
742372
Salmonella enterica
acetaldehyde + CoA + NAD+
-
-
-
r
acetyl-CoA + NADH + H+
low activity
742372
Listeria monocytogenes
acetaldehyde + CoA + NAD+
-
-
-
r
acetyl-CoA + NADH + H+
preferred substrate
742372
Listeria monocytogenes
acetaldehyde + CoA + NAD+
-
-
-
r
acetyl-CoA + NADH + H+
low activity
742372
Listeria monocytogenes M7
acetaldehyde + CoA + NAD+
-
-
-
r
acetyl-CoA + NADH + H+
preferred substrate
742372
Listeria monocytogenes M7
acetaldehyde + CoA + NAD+
-
-
-
r
butanoyl-CoA + NADH + H+
-
742372
Salmonella enterica
butanal + CoA + NAD+
-
-
-
r
butanoyl-CoA + NADH + H+
-
742372
Listeria monocytogenes
butanal + CoA + NAD+
-
-
-
r
butanoyl-CoA + NADH + H+
high activity
742372
Klebsiella pneumoniae
butanal + CoA + NAD+
-
-
-
r
butanoyl-CoA + NADH + H+
-
742372
Listeria monocytogenes M7
butanal + CoA + NAD+
-
-
-
r
additional information
substrate chain length specificity of the enzyme is C2-C12, overview
742372
Klebsiella pneumoniae
?
-
-
-
?
additional information
substrate chain length specificity of the enzyme is C2-C12, overview
742372
Salmonella enterica
?
-
-
-
?
additional information
substrate chain length specificity of the enzyme is C2-C12, overview
742372
Listeria monocytogenes
?
-
-
-
?
additional information
substrate chain length specificity of the enzyme is C2-C12, overview
742372
Listeria monocytogenes M7
?
-
-
-
?
propanoyl-CoA + NADH + H+
best substrate
742372
Klebsiella pneumoniae
propanal + CoA + NAD+
-
-
-
r
propanoyl-CoA + NADH + H+
best substrate
742372
Salmonella enterica
propanal + CoA + NAD+
-
-
-
r
propanoyl-CoA + NADH + H+
best substrate
742372
Listeria monocytogenes
propanal + CoA + NAD+
-
-
-
r
propanoyl-CoA + NADH + H+
best substrate
742372
Listeria monocytogenes M7
propanal + CoA + NAD+
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
CoA-acylating aldehyde dehydrogenase
-
Klebsiella pneumoniae
CoA-acylating aldehyde dehydrogenase
-
Salmonella enterica
CoA-acylating aldehyde dehydrogenase
-
Listeria monocytogenes
coenzyme A-acylating aldehyde dehydrogenase
-
Klebsiella pneumoniae
coenzyme A-acylating aldehyde dehydrogenase
-
Salmonella enterica
coenzyme A-acylating aldehyde dehydrogenase
-
Listeria monocytogenes
More
cf. EC 1.2.1.57
Salmonella enterica
PduP
-
Klebsiella pneumoniae
PduP
-
Salmonella enterica
PduP
-
Listeria monocytogenes
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Klebsiella pneumoniae
30
-
assay at
Salmonella enterica
30
-
assay at
Listeria monocytogenes
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.63
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
7.02
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
11.33
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
11.99
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
14.81
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
25.38
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.15
-
assay at
Klebsiella pneumoniae
7.15
-
assay at
Salmonella enterica
7.15
-
assay at
Listeria monocytogenes
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Klebsiella pneumoniae
NADH
-
Salmonella enterica
NADH
-
Listeria monocytogenes
Cloned(Commentary) (protein specific)
Commentary
Organism
gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Klebsiella pneumoniae produces more n-butanol than ethanol
Klebsiella pneumoniae
gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Listeria monocytogenes produces more ethanol than n-butanol
Listeria monocytogenes
gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Salmonella enterica produces more ethanol than n-butanol
Salmonella enterica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Klebsiella pneumoniae
NADH
-
Salmonella enterica
NADH
-
Listeria monocytogenes
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
design of a coenzyme A (CoA) dependent n-butanol biosynthesis pathway tailored to the metabolic physiology of the cyanobacterium Synechococcus elongatus PCC 7942 by incorporating an ATP driving force and a kinetically irreversible trap. Oxygen-sensitive CoA-acylating butyraldehyde dehydrogenase (Bldh) is exchanged for the oxygen-tolerant PduP from Salmonella enterica. Replacing Bldh with PduP in the n-butanol synthesis pathway results in n-butanol production to a cumulative titer of 404 mg/l with peak productivity of 51 mg/l per day, exceeding the base strain by 20fold. Anaerobic growth rescue of Escherichia coli strain JCL166 by overexpression of the Clostridium butanol pathway with different aldehyde dehydrogenases PduP
Salmonella enterica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kientics
Listeria monocytogenes
0.056
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
0.0717
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
0.087
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
0.0926
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
0.181
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
0.342
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
acetyl-CoA + NADH + H+
Klebsiella pneumoniae
-
acetaldehyde + CoA + NAD+
-
-
r
acetyl-CoA + NADH + H+
Salmonella enterica
-
acetaldehyde + CoA + NAD+
-
-
r
acetyl-CoA + NADH + H+
Listeria monocytogenes
low activity
acetaldehyde + CoA + NAD+
-
-
r
acetyl-CoA + NADH + H+
Listeria monocytogenes M7
low activity
acetaldehyde + CoA + NAD+
-
-
r
butanoyl-CoA + NADH + H+
Salmonella enterica
-
butanal + CoA + NAD+
-
-
r
butanoyl-CoA + NADH + H+
Listeria monocytogenes
-
butanal + CoA + NAD+
-
-
r
butanoyl-CoA + NADH + H+
Klebsiella pneumoniae
high activity
butanal + CoA + NAD+
-
-
r
butanoyl-CoA + NADH + H+
Listeria monocytogenes M7
-
butanal + CoA + NAD+
-
-
r
propanoyl-CoA + NADH + H+
Klebsiella pneumoniae
best substrate
propanal + CoA + NAD+
-
-
r
propanoyl-CoA + NADH + H+
Salmonella enterica
best substrate
propanal + CoA + NAD+
-
-
r
propanoyl-CoA + NADH + H+
Listeria monocytogenes
best substrate
propanal + CoA + NAD+
-
-
r
propanoyl-CoA + NADH + H+
Listeria monocytogenes M7
best substrate
propanal + CoA + NAD+
-
-
r
Oxidation Stability (protein specific)
Oxidation Stability
Organism
the enzyme is oxygen-tolerant
Klebsiella pneumoniae
the enzyme is oxygen-tolerant
Salmonella enterica
the enzyme is oxygen-tolerant
Listeria monocytogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.9
-
with butanoyl-CoA, pH 7.15, 30°C
Klebsiella pneumoniae
17
-
with butanoyl-CoA, pH 7.15, 30°C
Listeria monocytogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetyl-CoA + NADH + H+
-
742372
Klebsiella pneumoniae
acetaldehyde + CoA + NAD+
-
-
-
r
acetyl-CoA + NADH + H+
-
742372
Salmonella enterica
acetaldehyde + CoA + NAD+
-
-
-
r
acetyl-CoA + NADH + H+
low activity
742372
Listeria monocytogenes
acetaldehyde + CoA + NAD+
-
-
-
r
acetyl-CoA + NADH + H+
preferred substrate
742372
Listeria monocytogenes
acetaldehyde + CoA + NAD+
-
-
-
r
acetyl-CoA + NADH + H+
low activity
742372
Listeria monocytogenes M7
acetaldehyde + CoA + NAD+
-
-
-
r
acetyl-CoA + NADH + H+
preferred substrate
742372
Listeria monocytogenes M7
acetaldehyde + CoA + NAD+
-
-
-
r
butanoyl-CoA + NADH + H+
-
742372
Salmonella enterica
butanal + CoA + NAD+
-
-
-
r
butanoyl-CoA + NADH + H+
-
742372
Listeria monocytogenes
butanal + CoA + NAD+
-
-
-
r
butanoyl-CoA + NADH + H+
high activity
742372
Klebsiella pneumoniae
butanal + CoA + NAD+
-
-
-
r
butanoyl-CoA + NADH + H+
-
742372
Listeria monocytogenes M7
butanal + CoA + NAD+
-
-
-
r
additional information
substrate chain length specificity of the enzyme is C2-C12, overview
742372
Klebsiella pneumoniae
?
-
-
-
?
additional information
substrate chain length specificity of the enzyme is C2-C12, overview
742372
Salmonella enterica
?
-
-
-
?
additional information
substrate chain length specificity of the enzyme is C2-C12, overview
742372
Listeria monocytogenes
?
-
-
-
?
additional information
substrate chain length specificity of the enzyme is C2-C12, overview
742372
Listeria monocytogenes M7
?
-
-
-
?
propanoyl-CoA + NADH + H+
best substrate
742372
Klebsiella pneumoniae
propanal + CoA + NAD+
-
-
-
r
propanoyl-CoA + NADH + H+
best substrate
742372
Salmonella enterica
propanal + CoA + NAD+
-
-
-
r
propanoyl-CoA + NADH + H+
best substrate
742372
Listeria monocytogenes
propanal + CoA + NAD+
-
-
-
r
propanoyl-CoA + NADH + H+
best substrate
742372
Listeria monocytogenes M7
propanal + CoA + NAD+
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Klebsiella pneumoniae
30
-
assay at
Salmonella enterica
30
-
assay at
Listeria monocytogenes
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.63
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
7.02
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
11.33
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
11.99
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
14.81
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
25.38
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.15
-
assay at
Klebsiella pneumoniae
7.15
-
assay at
Salmonella enterica
7.15
-
assay at
Listeria monocytogenes
General Information
General Information
Commentary
Organism
metabolism
the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route
Salmonella enterica
metabolism
the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route
Listeria monocytogenes
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route
Salmonella enterica
metabolism
the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route
Listeria monocytogenes
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
25
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
43
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
122
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
125
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
167
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
292
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
25
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
43
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
122
-
acetyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
125
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Klebsiella pneumoniae
167
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Listeria monocytogenes
292
-
butanoyl-CoA
recombinant enzyme, pH 7.15, 30°C
Salmonella enterica
Other publictions for EC 1.2.1.87
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742372
Lan
-
Oxygen-tolerant coenzyme A-ac ...
Salmonella enterica, Klebsiella pneumoniae, Listeria monocytogenes, Listeria monocytogenes M7
Energy Environ. Sci.
6
2672-2681
2013
-
-
3
-
1
-
-
7
-
-
-
12
-
4
3
-
-
-
-
-
2
-
18
-
13
3
-
-
6
3
-
-
3
-
-
-
-
-
3
3
-
1
-
-
-
-
7
-
-
-
12
-
3
-
-
-
-
2
-
18
-
3
-
-
6
3
-
-
-
-
2
2
-
6
6
721659
Baker
Protein-protein interactions a ...
Thermus thermophilus
Biochemistry
51
1942-1952
2012
-
-
2
-
-
-
-
10
-
-
7
-
-
1
-
-
1
-
-
-
-
-
2
1
1
-
-
3
10
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
10
-
-
7
-
-
-
-
1
-
-
-
-
2
1
-
-
3
10
-
-
-
-
-
1
1
-
10
10
721651
Carere
Investigating the molecular de ...
Paraburkholderia xenovorans
Biochemistry
50
8407-8416
2011
-
-
1
-
2
-
-
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
1
1
-
-
-
721615
Baker
Characterization of an aldolas ...
Paraburkholderia xenovorans
Biochemistry
48
6551-6558
2009
-
-
1
-
-
-
-
6
-
-
3
1
-
1
-
-
1
-
-
-
-
1
8
1
1
-
-
-
6
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
6
-
-
3
1
-
-
-
1
-
-
-
1
8
1
-
-
-
6
-
-
-
-
-
1
1
-
7
7