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Literature summary for 1.2.1.87 extracted from

  • Lan, E.; Ro, S.; Liao, J.
    Oxygen-tolerant coenzyme A-acylating aldehyde dehydrogenase facilitates efficient photosynthetic n-butanol biosynthesis in cyanobacteria (2013), Energy Environ. Sci., 6, 2672-2681 .
No PubMed abstract available
Search for more literature for 1.2.1.87

Cloned(Commentary)

Cloned (Comment) Organism
gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Klebsiella pneumoniae produces more n-butanol than ethanol Klebsiella pneumoniae
gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Listeria monocytogenes produces more ethanol than n-butanol Listeria monocytogenes
gene pduP, recombinant expression in Escherichia coli strain JCL166, strain JCL166 cannot grow anaerobically unless complemented by an exogenous fermentation pathway such as n-butanol biosynthesis. Recombinant coexpression of PduP with the enzymes of the n-butanol synthesis pathway in Synechococcus elongatus strain PCC 7942 results in autotrophic n-butanol production. PduP from Salmonella enterica produces more ethanol than n-butanol Salmonella enterica

Protein Variants

Protein Variants Comment Organism
additional information design of a coenzyme A (CoA) dependent n-butanol biosynthesis pathway tailored to the metabolic physiology of the cyanobacterium Synechococcus elongatus PCC 7942 by incorporating an ATP driving force and a kinetically irreversible trap. Oxygen-sensitive CoA-acylating butyraldehyde dehydrogenase (Bldh) is exchanged for the oxygen-tolerant PduP from Salmonella enterica. Replacing Bldh with PduP in the n-butanol synthesis pathway results in n-butanol production to a cumulative titer of 404 mg/l with peak productivity of 51 mg/l per day, exceeding the base strain by 20fold. Anaerobic growth rescue of Escherichia coli strain JCL166 by overexpression of the Clostridium butanol pathway with different aldehyde dehydrogenases PduP Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kientics Listeria monocytogenes
0.056
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
0.0717
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
0.087
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica
0.0926
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
0.181
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
0.342
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + NADH + H+ Klebsiella pneumoniae
-
 acetaldehyde + CoA + NAD+
-
 r
acetyl-CoA + NADH + H+ Salmonella enterica
-
 acetaldehyde + CoA + NAD+
-
 r
acetyl-CoA + NADH + H+ Listeria monocytogenes low activity acetaldehyde + CoA + NAD+
-
 r
acetyl-CoA + NADH + H+ Listeria monocytogenes M7 low activity acetaldehyde + CoA + NAD+
-
 r
butanoyl-CoA + NADH + H+ Salmonella enterica
-
 butanal + CoA + NAD+
-
 r
butanoyl-CoA + NADH + H+ Listeria monocytogenes
-
 butanal + CoA + NAD+
-
 r
butanoyl-CoA + NADH + H+ Klebsiella pneumoniae high activity butanal + CoA + NAD+
-
 r
butanoyl-CoA + NADH + H+ Listeria monocytogenes M7
-
 butanal + CoA + NAD+
-
 r
propanoyl-CoA + NADH + H+ Klebsiella pneumoniae best substrate propanal + CoA + NAD+
-
 r
propanoyl-CoA + NADH + H+ Salmonella enterica best substrate propanal + CoA + NAD+
-
 r
propanoyl-CoA + NADH + H+ Listeria monocytogenes best substrate propanal + CoA + NAD+
-
 r
propanoyl-CoA + NADH + H+ Listeria monocytogenes M7 best substrate propanal + CoA + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Listeria monocytogenes A0A0E0UUX4
-
-
Listeria monocytogenes M7 A0A0E0UUX4
-
-
Salmonella enterica
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is oxygen-tolerant Klebsiella pneumoniae
the enzyme is oxygen-tolerant Salmonella enterica
the enzyme is oxygen-tolerant Listeria monocytogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.9
-
 with butanoyl-CoA, pH 7.15, 30°C Klebsiella pneumoniae
17
-
 with butanoyl-CoA, pH 7.15, 30°C Listeria monocytogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + NADH + H+
-
 Klebsiella pneumoniae acetaldehyde + CoA + NAD+
-
 r
acetyl-CoA + NADH + H+
-
 Salmonella enterica acetaldehyde + CoA + NAD+
-
 r
acetyl-CoA + NADH + H+ low activity Listeria monocytogenes acetaldehyde + CoA + NAD+
-
 r
acetyl-CoA + NADH + H+ preferred substrate Listeria monocytogenes acetaldehyde + CoA + NAD+
-
 r
acetyl-CoA + NADH + H+ low activity Listeria monocytogenes M7 acetaldehyde + CoA + NAD+
-
 r
acetyl-CoA + NADH + H+ preferred substrate Listeria monocytogenes M7 acetaldehyde + CoA + NAD+
-
 r
butanoyl-CoA + NADH + H+
-
 Salmonella enterica butanal + CoA + NAD+
-
 r
butanoyl-CoA + NADH + H+
-
 Listeria monocytogenes butanal + CoA + NAD+
-
 r
butanoyl-CoA + NADH + H+ high activity Klebsiella pneumoniae butanal + CoA + NAD+
-
 r
butanoyl-CoA + NADH + H+
-
 Listeria monocytogenes M7 butanal + CoA + NAD+
-
 r
additional information substrate chain length specificity of the enzyme is C2-C12, overview Klebsiella pneumoniae ?
-
 ?
additional information substrate chain length specificity of the enzyme is C2-C12, overview Salmonella enterica ?
-
 ?
additional information substrate chain length specificity of the enzyme is C2-C12, overview Listeria monocytogenes ?
-
 ?
additional information substrate chain length specificity of the enzyme is C2-C12, overview Listeria monocytogenes M7 ?
-
 ?
propanoyl-CoA + NADH + H+ best substrate Klebsiella pneumoniae propanal + CoA + NAD+
-
 r
propanoyl-CoA + NADH + H+ best substrate Salmonella enterica propanal + CoA + NAD+
-
 r
propanoyl-CoA + NADH + H+ best substrate Listeria monocytogenes propanal + CoA + NAD+
-
 r
propanoyl-CoA + NADH + H+ best substrate Listeria monocytogenes M7 propanal + CoA + NAD+
-
 r

Synonyms

Synonyms Comment Organism
CoA-acylating aldehyde dehydrogenase
-
 Klebsiella pneumoniae
CoA-acylating aldehyde dehydrogenase
-
 Salmonella enterica
CoA-acylating aldehyde dehydrogenase
-
 Listeria monocytogenes
coenzyme A-acylating aldehyde dehydrogenase
-
 Klebsiella pneumoniae
coenzyme A-acylating aldehyde dehydrogenase
-
 Salmonella enterica
coenzyme A-acylating aldehyde dehydrogenase
-
 Listeria monocytogenes
More cf. EC 1.2.1.57 Salmonella enterica
PduP
-
 Klebsiella pneumoniae
PduP
-
 Salmonella enterica
PduP
-
 Listeria monocytogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Klebsiella pneumoniae
30
-
 assay at Salmonella enterica
30
-
 assay at Listeria monocytogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.63
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
7.02
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
11.33
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
11.99
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
14.81
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica
25.38
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.15
-
 assay at Klebsiella pneumoniae
7.15
-
 assay at Salmonella enterica
7.15
-
 assay at Listeria monocytogenes

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Klebsiella pneumoniae
NADH
-
 Salmonella enterica
NADH
-
 Listeria monocytogenes

General Information

General Information Comment Organism
metabolism the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route Salmonella enterica
metabolism the oxygen sensitivity of CoA-acylating aldehyde dehydrogenase appears to be a key limiting factor for cyanobacteria to produce alcohols through the CoA-dependent route Listeria monocytogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
25
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
43
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica
122
-
 acetyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
125
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Klebsiella pneumoniae
167
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Listeria monocytogenes
292
-
 butanoyl-CoA recombinant enzyme, pH 7.15, 30°C Salmonella enterica