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Literature summary for 1.2.1.84 extracted from

  • Zhang, S.; Wu, S.; Niu, C.; Liu, D.; Yan, T.; Tian, Y.; Liu, S.; Xie, K.; Li, Z.; Wang, Y.; Zhao, W.; Dong, Z.; Zhu, T.; Hou, Q.; Ma, B.; An, X.; Li, J.; Wan, X.
    ZmMs25 encoding a plastid-localized fatty acyl reductase is critical for anther and pollen development in maize (2021), J. Exp. Bot., 72, 4298-4318 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Zea mays

Protein Variants

Protein Variants Comment Organism
G101A the activity of the variant is decreased by 62.2% after incubation with short-chain lauroyl-CoA at pH 8.0 and 30°C for 180 min, but 100% activity is lost when using long-chain palmitoyl- and stearoyl-CoAs as the substrate Zea mays
G104A the activities of the variant are decreased by 56.1%, 65.9%, and 68.3% toward lauroyl-, palmitoyl-, and stearoyl-CoAs, respectively after 180 min incubation, compared with the wild type enzyme Zea mays
Y327F the activities of the variant drop to 0-6.6% toward lauroyl-, palmitoyl-, and stearoyl-CoAs after 180 min incubation, compared with the wild type enzyme Zea mays
Y331I the activities of the variant are decreased by 86.2%, 88.3%, and 93.2% toward lauroyl-, palmitoyl-, and stearoyl-CoAs, respectively, compared with the wild type enzyme Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Zea mays 9536
-

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni affinity column chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
anther
-
 Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lauroyl-CoA + 2 NADPH + 2 H+
-
 Zea mays dodecanal + CoA + 2 NADP+
-
 ?
palmitoyl-CoA + 2 NADPH + 2 H+
-
 Zea mays hexadecanal + CoA + 2 NADP+
-
 ?
stearoyl-CoA + 2 NADPH + 2 H+
-
 Zea mays octadecanal + CoA + 2 NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 106000, maltose-binding protein-tagged enzyme, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
Ms25
-
 Zea mays

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Zea mays

Expression

Organism Comment Expression
Zea mays the enzyme gene is directly activated and regulated by MYB84 up

General Information

General Information Comment Organism
malfunction enzyme mutation has significant effects on reducing cutin monomers and internal lipids, and altering the composition of cuticular wax in anthers. Moreover, loss of function of the enzyme significantly affects the expression of its four paralogous genes and five cloned lipid metabolic male-sterility genes in maize Zea mays
physiological function the enzyme is critical for anther and pollen development in maize Zea mays