Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose 6 column chromatography | Rhodococcus jostii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0015 | - |
with lauroyl-CoA as substrate, at pH 7.0 and 25°C | Rhodococcus jostii |
0.007 | - |
with palmitoyl-CoA as substrate, at pH 7.0 and 25°C | Rhodococcus jostii |
0.041 | - |
with oleoyl-CoA as substrate, at pH 7.0 and 25°C | Rhodococcus jostii |
0.045 | - |
with stearoyl-CoA as substrate, at pH 7.0 and 25°C | Rhodococcus jostii |
2.8 | - |
with cis-11-hexadecanal as substrate, at pH 7.0 and 25°C | Rhodococcus jostii |
3.3 | - |
with decanal as substrate, at pH 7.0 and 25°C | Rhodococcus jostii |
5.3 | - |
with dodecanal as substrate, at pH 7.0 and 25°C | Rhodococcus jostii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-11-hexadecenal + NADPH + H+ | - |
Rhodococcus jostii | cis-11-hexadecenol + NADP+ | - |
? | |
decanal + NADPH + H+ | - |
Rhodococcus jostii | decanol + NADP+ | - |
? | |
dodecanal + NADPH + H+ | - |
Rhodococcus jostii | dodecanol + NADP+ | - |
? | |
lauroyl-CoA + 2 NADPH + 2 H+ | - |
Rhodococcus jostii | dodecanol + CoA + 2 NADP+ | - |
? | |
additional information | no activity is detected when NADPH is substituted with NADH | Rhodococcus jostii | ? | - |
- |
|
oleoyl-CoA + 2 NADPH + 2 H+ | - |
Rhodococcus jostii | octadecenol + CoA + 2 NADP+ | - |
? | |
palmitoyl-CoA + 2 NADPH + 2 H+ | - |
Rhodococcus jostii | hexadecanol + CoA + 2 NADP+ | - |
? | |
stearoyl-CoA + 2 NADPH + 2 H+ | - |
Rhodococcus jostii | stearyl alcohol + CoA + 2 NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 73627, calculated from amino acid sequence | Rhodococcus jostii |
? | x * 75000, His6-tagged enzyme, SDS-PAGE | Rhodococcus jostii |
Synonyms | Comment | Organism |
---|---|---|
alcohol-forming fatty acyl-CoA reductase | - |
Rhodococcus jostii |
fatty acyl coenzyme A reductase | - |
Rhodococcus jostii |
FcrA | - |
Rhodococcus jostii |
RS30405 | - |
Rhodococcus jostii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rhodococcus jostii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme promotes wax ester accumulation in Rhodococcus jostii RHA1 | Rhodococcus jostii |