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Literature summary for 1.2.1.84 extracted from

  • Miklaszewska, M.; Banas, A.
    Biochemical characterization and substrate specificity of jojoba fatty acyl-CoA reductase and jojoba wax synthase (2016), Plant Sci., 249, 84-92 .
    View publication on PubMed
Search for more literature for 1.2.1.84

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activates 4fold at 0.5 mg Simmondsia chinensis

Cloned(Commentary)

Cloned (Comment) Organism
gene FAR, recombinant expression in Saccharomyces cerevisiae wild-type strain BY4742 and in quadruple mutant strain H1246 lacking genes involved in triacylglycerolsand sterol esters synthesis Simmondsia chinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane a membrane-associated enzyme Simmondsia chinensis 16020
-
microsome
-
 Simmondsia chinensis
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a long-chain acyl-CoA + 2 NADPH + 2 H+ Simmondsia chinensis
-
 a long-chain alcohol + 2 NADP+ + coenzyme A
-
 ?

Organism

Organism UniProt Comment Textmining
Simmondsia chinensis Q9XGY7
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by microsomal membrane preparation Simmondsia chinensis

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Simmondsia chinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylhexadecanoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis 2-methylhexadecanol + 2 NADP+ + coenzyme A
-
 ?
2-methyloctadecanoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis 2-methyloctadecanol + 2 NADP+ + coenzyme A
-
 ?
a long-chain acyl-CoA + 2 NADPH + 2 H+
-
 Simmondsia chinensis a long-chain alcohol + 2 NADP+ + coenzyme A
-
 ?
arachidoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis arachidol + 2 NADP+ + coenzyme A
-
 ?
decanoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis decanol + 2 NADP+ + coenzyme A
-
 ?
eicosenoyl-CoA + 2 NADPH + 2 H+ higher activity Simmondsia chinensis eicosanol + 2 NADP+ + coenzyme A
-
 ?
erucyl-CoA + 2 NADPH + 2 H+ moderate activity Simmondsia chinensis erucyl alcohol + 2 NADP+ + coenzyme A
-
 ?
lauroyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis dodecanol + 2 NADP+ + coenzyme A
-
 ?
additional information enzyme substrate specificity, overview. Poor activity with linoleoyl-CoA and linolenoyl-CoA Simmondsia chinensis ?
-
 ?
myristoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis myristol + 2 NADP+ + coenzyme A
-
 ?
oleoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis octadecenol + 2 NADP+ + coenzyme A
-
 ?
palmitoyl-CoA + 2 NADPH + 2 H+ lower activity Simmondsia chinensis hexadecanol + 2 NADP+ + coenzyme A
-
 ?
ricinoleoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis (9Z,12R)-octadec-9-ene-1,12-diol + 2 NADP+ + coenzyme A
-
 ?
stearoyl-CoA + 2 NADPH + 2 H+ best substrate Simmondsia chinensis octadecanol + 2 NADP+ + coenzyme A
-
 ?

Subunits

Subunits Comment Organism
? x * 56200, about, sequence calculation Simmondsia chinensis
More alcohol-forming fatty acyl-CoA reductases are membrane-associated enzymes, which contain a Rossmann fold NAD(P)H-binding domain and a fatty acyl-CoA reductase (FAR C) domain Simmondsia chinensis

Synonyms

Synonyms Comment Organism
alcohol-forming fatty acyl-CoA reductase
-
 Simmondsia chinensis
FAR
-
 Simmondsia chinensis
fatty acyl-CoA reductase
-
 Simmondsia chinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Simmondsia chinensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 over 50% of maximal activity within this range, 50% of maximal activity at 25°C Simmondsia chinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Simmondsia chinensis

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5 inactive at pH 4.0, gradual increase up to maximal activity at pH 6.5, 42% of maximal activity at pH 7.5, reduction of activity above Simmondsia chinensis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Simmondsia chinensis