BRENDA - Enzyme Database
show all sequences of 1.2.1.83

Functional identification of two novel genes from Pseudomonas sp. strain HZN6 involved in the catabolism of nicotine

Qiu, J.; Ma, Y.; Wen, Y.; Chen, L.; Wu, L.; Liu, W.; Appl. Environ. Microbiol. 78, 2154-2160 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Pseudomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
H8ZPX2
-
-
Pseudomonas sp. HZN6
H8ZPX2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-oxo-4-(pyridin-3-yl)butanal + NADP+ + H2O
-
718644
Pseudomonas sp.
4-oxo-4-(pyridin-3-yl)butanoate + NADPH + H+
-
-
-
?
4-oxo-4-(pyridin-3-yl)butanal + NADP+ + H2O
-
718644
Pseudomonas sp. HZN6
4-oxo-4-(pyridin-3-yl)butanoate + NADPH + H+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
ALD
-
Pseudomonas sp.
SapD
-
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NAD+
Pseudomonas sp.
NADP+
specific for NADP+
Pseudomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NAD+
Pseudomonas sp.
NADP+
specific for NADP+
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-oxo-4-(pyridin-3-yl)butanal + NADP+ + H2O
-
718644
Pseudomonas sp.
4-oxo-4-(pyridin-3-yl)butanoate + NADPH + H+
-
-
-
?
4-oxo-4-(pyridin-3-yl)butanal + NADP+ + H2O
-
718644
Pseudomonas sp. HZN6
4-oxo-4-(pyridin-3-yl)butanoate + NADPH + H+
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
an enzyme deletion strain is still able to grow on pseudoxynicotine, the cell growth and the rate of pseudoxynicotine removal are initially slightly reduced. After the deletion strain is cultured with pseudoxynicotine for several generations, the pseudoxynicotine degradation characteristics reach the wild-type level
Pseudomonas sp.
General Information (protein specific)
General Information
Commentary
Organism
physiological function
an enzyme deletion strain is still able to grow on pseudoxynicotine, the cell growth and the rate of pseudoxynicotine removal are initially slightly reduced. After the deletion strain is cultured with pseudoxynicotine for several generations, the pseudoxynicotine degradation characteristics reach the wild-type level
Pseudomonas sp.
Other publictions for EC 1.2.1.83
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718644
Qiu
Functional identification of t ...
Pseudomonas sp., Pseudomonas sp. HZN6
Appl. Environ. Microbiol.
78
2154-2160
2012
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