BRENDA - Enzyme Database
show all sequences of 1.2.1.82

The gene carD encodes the aldehyde dehydrogenase responsible for neurosporaxanthin biosynthesis in Fusarium fujikuroi

Diaz-Sanchez, V.; Estrada, A.F.; Trautmann, D.; Al-Babili, S.; Avalos, J.; FEBS J. 278, 3164-3176 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
carD expressed in Escherichia coli BL21 strain
Fusarium fujikuroi
generation of an Escherichia coli strain producing beta-apo-4'-carotenal, which is converted into neurosporaxanthin upon expression of carD
Fusarium fujikuroi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
beta-apo-4'-carotenal + NAD+ + H2O
Fusarium fujikuroi
carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal
beta-apo-4'-carotenoic acid + NADH + H+
beta-apo-4'-carotenoic acid i.e. neurosporaxanthin
-
?
Organism
Organism
UniProt
Commentary
Textmining
Fusarium fujikuroi
F6IBC7
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial purification by generating of a crude lysate for in vitro assays
Fusarium fujikuroi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
apo-8'-lycopenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
apo-8'-lycopenoic acid + NADH + 2 H+
-
-
-
?
apo-8'-lycopenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
apo-8'-lycopenoic acid + NADH + H+
-
-
-
?
beta-apo-10'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-10'-carotenoic acid + NADH + H+
-
-
-
?
beta-apo-10'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-10'-carotenoic acid + NADH + 2 H+
-
-
-
?
beta-apo-4'-carotenal + NAD+ + H2O
carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal
714988
Fusarium fujikuroi
beta-apo-4'-carotenoic acid + NADH + H+
beta-apo-4'-carotenoic acid i.e. neurosporaxanthin
-
-
?
beta-apo-4'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
neurosporaxanthin + NADH + 2 H+
-
-
-
?
beta-apo-8'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-8'-carotenoic acid + NADH + H+
-
-
-
?
beta-apo-8'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-8'-carotenoic acid + NADH + 2 H+
-
-
-
?
additional information
no substrate: retinal
714988
Fusarium fujikuroi
?
-
-
-
?
additional information
C20-retinal i.e. beta-apo-15'-carotenal is not converted by carD enzymatic activity, indicating the requirement for a minimal length of the substrate chain
714988
Fusarium fujikuroi
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CarD
-
Fusarium fujikuroi
Cloned(Commentary) (protein specific)
Commentary
Organism
carD expressed in Escherichia coli BL21 strain
Fusarium fujikuroi
generation of an Escherichia coli strain producing beta-apo-4'-carotenal, which is converted into neurosporaxanthin upon expression of carD
Fusarium fujikuroi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
beta-apo-4'-carotenal + NAD+ + H2O
Fusarium fujikuroi
carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal
beta-apo-4'-carotenoic acid + NADH + H+
beta-apo-4'-carotenoic acid i.e. neurosporaxanthin
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification by generating of a crude lysate for in vitro assays
Fusarium fujikuroi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
apo-8'-lycopenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
apo-8'-lycopenoic acid + NADH + 2 H+
-
-
-
?
apo-8'-lycopenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
apo-8'-lycopenoic acid + NADH + H+
-
-
-
?
beta-apo-10'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-10'-carotenoic acid + NADH + H+
-
-
-
?
beta-apo-10'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-10'-carotenoic acid + NADH + 2 H+
-
-
-
?
beta-apo-4'-carotenal + NAD+ + H2O
carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal
714988
Fusarium fujikuroi
beta-apo-4'-carotenoic acid + NADH + H+
beta-apo-4'-carotenoic acid i.e. neurosporaxanthin
-
-
?
beta-apo-4'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
neurosporaxanthin + NADH + 2 H+
-
-
-
?
beta-apo-8'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-8'-carotenoic acid + NADH + H+
-
-
-
?
beta-apo-8'-carotenal + NAD+ + H2O
-
714988
Fusarium fujikuroi
beta-apo-8'-carotenoic acid + NADH + 2 H+
-
-
-
?
additional information
no substrate: retinal
714988
Fusarium fujikuroi
?
-
-
-
?
additional information
C20-retinal i.e. beta-apo-15'-carotenal is not converted by carD enzymatic activity, indicating the requirement for a minimal length of the substrate chain
714988
Fusarium fujikuroi
?
-
-
-
?
Expression
Organism
Commentary
Expression
Fusarium fujikuroi
CarD mRNA content is enhanced by light, and CarD mRNA levels are higher in carotenoid-overproducing mutants
up
Fusarium fujikuroi
carD mRNA levels increase about 3fold after 30 min of illumination
up
General Information
General Information
Commentary
Organism
physiological function
targeted disruption of CarD in a Gibberella fujikuroi carotenoid-overproducing strain results in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal, its derivative beta-apo-4'-carotenol, and minor amounts of other carotenoids. Strains harboring the CarD mutation acquire a yellowish pigmentation
Fusarium fujikuroi
General Information (protein specific)
General Information
Commentary
Organism
physiological function
targeted disruption of CarD in a Gibberella fujikuroi carotenoid-overproducing strain results in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal, its derivative beta-apo-4'-carotenol, and minor amounts of other carotenoids. Strains harboring the CarD mutation acquire a yellowish pigmentation
Fusarium fujikuroi
Expression (protein specific)
Organism
Commentary
Expression
Fusarium fujikuroi
CarD mRNA content is enhanced by light, and CarD mRNA levels are higher in carotenoid-overproducing mutants
up
Fusarium fujikuroi
carD mRNA levels increase about 3fold after 30 min of illumination
up
Other publictions for EC 1.2.1.82
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714988
Diaz-Sanchez
The gene carD encodes the alde ...
Fusarium fujikuroi
FEBS J.
278
3164-3176
2011
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-
2
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
10
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
698070
Estrada
Novel apocarotenoid intermedia ...
Neurospora crassa
Fungal Genet. Biol.
45
1497-1505
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
700267
Estrada
The ylo-1 gene encodes an alde ...
Neurospora crassa
Mol. Microbiol.
69
1207-1220
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
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5
-
1
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-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
716451
Sandmann
C35-apocarotenoids in the yell ...
Neurospora crassa
Phytochemistry
69
2886-2890
2008
-
-
-
-
-
-
-
-
-
-
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-
-
4
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1
1
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