BRENDA - Enzyme Database
show all sequences of 1.2.1.82

Novel apocarotenoid intermediates in Neurospora crassa mutants imply a new biosynthetic reaction sequence leading to neurosporaxanthin formation

Estrada, A.F.; Maier, D.; Scherzinger, D.; Avalos, J.; Al-Babili, S.; Fungal Genet. Biol. 45, 1497-1505 (2008)

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Neurospora crassa
Q1K615
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
apo-4'-lycopenal + NAD+ + H2O
the in vivo substrate of Ylo-1 is apo-4'-lycopenal rather than its cyclic isomer beta-apo-4'-carotenal
698070
Neurospora crassa
apo-4'-lycopenoic acid + NADH + 2 H+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
YLO-1
-
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
apo-4'-lycopenal + NAD+ + H2O
the in vivo substrate of Ylo-1 is apo-4'-lycopenal rather than its cyclic isomer beta-apo-4'-carotenal
698070
Neurospora crassa
apo-4'-lycopenoic acid + NADH + 2 H+
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
enzyme is involved in the biosynthetic pathway to neurosporaxanthin. First step is the cleavage of 3,4-didehydrolycopene into apo-4'-lycopenal by Cao2, which is then oxidized by YLO-1 to yield apo-4'-lycopenoic acid, the last intermediate in the pathway. The final step is then catalyzed by the cyclase activity of AL-2. An Ylo-1 mutant accumulates three major pigments, i.e. lycopene, apo-4'-lycopenal and apo-4'-lycopenol. The pigments accumulating in the Ylo-1 mutant are non-cyclic and represent lycopene and apolycopenoids, indicating that the in vivo substrate of Ylo-1 is apo-4'-lycopenal rather than its cyclic isomer beta-apo-4'-carotenal
Neurospora crassa
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzyme is involved in the biosynthetic pathway to neurosporaxanthin. First step is the cleavage of 3,4-didehydrolycopene into apo-4'-lycopenal by Cao2, which is then oxidized by YLO-1 to yield apo-4'-lycopenoic acid, the last intermediate in the pathway. The final step is then catalyzed by the cyclase activity of AL-2. An Ylo-1 mutant accumulates three major pigments, i.e. lycopene, apo-4'-lycopenal and apo-4'-lycopenol. The pigments accumulating in the Ylo-1 mutant are non-cyclic and represent lycopene and apolycopenoids, indicating that the in vivo substrate of Ylo-1 is apo-4'-lycopenal rather than its cyclic isomer beta-apo-4'-carotenal
Neurospora crassa
Other publictions for EC 1.2.1.82
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714988
Diaz-Sanchez
The gene carD encodes the alde ...
Fusarium fujikuroi
FEBS J.
278
3164-3176
2011
-
-
2
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
10
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
698070
Estrada
Novel apocarotenoid intermedia ...
Neurospora crassa
Fungal Genet. Biol.
45
1497-1505
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
700267
Estrada
The ylo-1 gene encodes an alde ...
Neurospora crassa
Mol. Microbiol.
69
1207-1220
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
716451
Sandmann
C35-apocarotenoids in the yell ...
Neurospora crassa
Phytochemistry
69
2886-2890
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-