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show all sequences of 1.2.1.80

Efficient delivery of long-chain fatty aldehydes from the Nostoc punctiforme acyl-acyl carrier protein reductase to its cognate aldehyde-deformylating oxygenase

Warui, D.M.; Pandelia, M.E.; Rajakovich, L.J.; Krebs, C.; Bollinger, J.M.; Booker, S.J.; Biochemistry 54, 1006-1015 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expession in Escherichia coli
Nostoc punctiforme
Inhibitors
Inhibitors
Commentary
Organism
Structure
K+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations
Nostoc punctiforme
NH4+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations
Nostoc punctiforme
Rb+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations
Nostoc punctiforme
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
stearoyl-[acyl-carrier protein]
presence of potassium ions, pH 7.6, 37C
Nostoc punctiforme
0.027
-
stearoyl-[acyl-carrier protein]
pH 7.6, 37C
Nostoc punctiforme
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations
Nostoc punctiforme
NH4+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations
Nostoc punctiforme
Rb+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations
Nostoc punctiforme
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nostoc punctiforme
B2J1M0
-
-
Nostoc punctiforme ATCC 29133
B2J1M0
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
commercial preparation
codon-optimized gene for expression in Escherichia coli
Nostoc punctiforme
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
stearoyl-[acyl-carrier protein] + NAD(P)H + H+
-
740064
Nostoc punctiforme
stearylaldehyde + an [acyl-carrier protein] + NAD(P)+
the enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product
-
-
?
stearoyl-[acyl-carrier protein] + NAD(P)H + H+
-
740064
Nostoc punctiforme ATCC 29133
stearylaldehyde + an [acyl-carrier protein] + NAD(P)+
the enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0003
-
stearoyl-[acyl-carrier protein]
pH 7.6, 37C
Nostoc punctiforme
0.0028
-
stearoyl-[acyl-carrier protein]
presence of potassium ions, pH 7.6, 37C
Nostoc punctiforme
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Nostoc punctiforme
Cloned(Commentary) (protein specific)
Commentary
Organism
expession in Escherichia coli
Nostoc punctiforme
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Nostoc punctiforme
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
K+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations
Nostoc punctiforme
NH4+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations
Nostoc punctiforme
Rb+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations
Nostoc punctiforme
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
stearoyl-[acyl-carrier protein]
presence of potassium ions, pH 7.6, 37C
Nostoc punctiforme
0.027
-
stearoyl-[acyl-carrier protein]
pH 7.6, 37C
Nostoc punctiforme
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations
Nostoc punctiforme
NH4+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations
Nostoc punctiforme
Rb+
K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations
Nostoc punctiforme
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
commercial preparation
codon-optimized gene for expression in Escherichia coli
Nostoc punctiforme
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
stearoyl-[acyl-carrier protein] + NAD(P)H + H+
-
740064
Nostoc punctiforme
stearylaldehyde + an [acyl-carrier protein] + NAD(P)+
the enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product
-
-
?
stearoyl-[acyl-carrier protein] + NAD(P)H + H+
-
740064
Nostoc punctiforme ATCC 29133
stearylaldehyde + an [acyl-carrier protein] + NAD(P)+
the enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0003
-
stearoyl-[acyl-carrier protein]
pH 7.6, 37C
Nostoc punctiforme
0.0028
-
stearoyl-[acyl-carrier protein]
presence of potassium ions, pH 7.6, 37C
Nostoc punctiforme
General Information
General Information
Commentary
Organism
physiological function
enzyme and aldehyde-deformylating oxygenase ADO form a tight isolable complex with a Kd of 3 microM. When the aldehyde substrate is supplied to ADO by acyl-acyl carrier protein reductase, efficient in vitro turnover is observed in the absence of solubilizing agents. Catalysis by acyl-acyl carrier protein reductase proceeds via formation of a covalent intermediate involving residue C294. The enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product
Nostoc punctiforme
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzyme and aldehyde-deformylating oxygenase ADO form a tight isolable complex with a Kd of 3 microM. When the aldehyde substrate is supplied to ADO by acyl-acyl carrier protein reductase, efficient in vitro turnover is observed in the absence of solubilizing agents. Catalysis by acyl-acyl carrier protein reductase proceeds via formation of a covalent intermediate involving residue C294. The enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product
Nostoc punctiforme
Other publictions for EC 1.2.1.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740064
Warui
Efficient delivery of long-cha ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133
Biochemistry
54
1006-1015
2015
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1
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724441
Doan
Biochemical characterization o ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1821
1244-1255
2012
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8
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713511
Schirmer
Microbial biosynthesis of alka ...
Synechococcus elongatus, Synechococcus sp., Synechococcus sp. PCC 7942
Science
329
559-562
2010
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1
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1
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1
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3
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22
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7
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7
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724655
Zhu
The reductase domain in a Type ...
Cryptosporidium parvum
BMC Biochem.
11
46
2010
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1
1
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1
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1
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4
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4
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1
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1
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