BRENDA - Enzyme Database
show all sequences of 1.2.1.80

The reductase domain in a Type i fatty acid synthase from the apicomplexan Cryptosporidium parvum: Restricted substrate preference towards very long chain fatty acyl thioesters

Zhu, G.; Shi, X.; Cai, X.; BMC Biochem. 11, 46 (2010)

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
since the giant CpFAS1 and polyketide synthase CpPKS1 are structurally and functionally different from human Type I FAS, these parasite megasynthases may serve as novel drug targets, also because CpFAS1 and CpPKS1 utilize R domains to release final products
Cryptosporidium parvum
Cloned(Commentary)
Cloned (Commentary)
Organism
in a Rosetta bacterial strain serving as an expression host for the maltose-binding protein-CpFAS1-R fusion protein
Cryptosporidium parvum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.91
-
hexacosanoyl-CoA
pH 7.2, temperature not specified in the publication
Cryptosporidium parvum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
fatty acyl-[acyl-carrier protein] + NAD(P)H + H+
Cryptosporidium parvum
-
fatty aldehyde + acyl-carrier protein + NAD(P)+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Cryptosporidium parvum
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
to homogeneity as a maltose-binding protein-fusion protein by amylose-resin based affinity chromatography
Cryptosporidium parvum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
fatty acyl-CoA + NAD(P)H + H+
CpFAS1-R can only utilize very long chain fatty acyl-CoAs as substrates, with activity on C26 > C24 > C22 > C20, but no activity on C18 and C16 or fewer carbons
724655
Cryptosporidium parvum
fatty aldehyde + CoA + NAD(P)+
since aldehydes are toxic to cells it is very likely that the fatty aldehyde is immediately transformed into the corresponding alcohol by the enzyme complex
-
-
?
fatty acyl-[acyl-carrier protein] + NAD(P)H + H+
-
724655
Cryptosporidium parvum
fatty aldehyde + acyl-carrier protein + NAD(P)+
-
-
-
?
hexacosanoyl-CoA + NAD(P)H + H+
-
724655
Cryptosporidium parvum
hexacosanal + CoA + NAD(P)+
-
-
-
?
additional information
because of technical difficulties in preparing the native substrates for CpFAS1-R, which are very long chain fatty acyl-ACPs, long chain and very long chain fatty acyl-CoAs are used as substrates to assay CpFAS1-R activity
724655
Cryptosporidium parvum
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CpFAS1 acyl reductase
-
Cryptosporidium parvum
CpFAS1-R
-
Cryptosporidium parvum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.077
-
hexacosanoyl-CoA
pH 7.2, temperature not specified in the publication
Cryptosporidium parvum
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Cryptosporidium parvum
NADPH
preferred cofactor
Cryptosporidium parvum
Application (protein specific)
Application
Commentary
Organism
drug development
since the giant CpFAS1 and polyketide synthase CpPKS1 are structurally and functionally different from human Type I FAS, these parasite megasynthases may serve as novel drug targets, also because CpFAS1 and CpPKS1 utilize R domains to release final products
Cryptosporidium parvum
Cloned(Commentary) (protein specific)
Commentary
Organism
in a Rosetta bacterial strain serving as an expression host for the maltose-binding protein-CpFAS1-R fusion protein
Cryptosporidium parvum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Cryptosporidium parvum
NADPH
preferred cofactor
Cryptosporidium parvum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.91
-
hexacosanoyl-CoA
pH 7.2, temperature not specified in the publication
Cryptosporidium parvum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
fatty acyl-[acyl-carrier protein] + NAD(P)H + H+
Cryptosporidium parvum
-
fatty aldehyde + acyl-carrier protein + NAD(P)+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity as a maltose-binding protein-fusion protein by amylose-resin based affinity chromatography
Cryptosporidium parvum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
fatty acyl-CoA + NAD(P)H + H+
CpFAS1-R can only utilize very long chain fatty acyl-CoAs as substrates, with activity on C26 > C24 > C22 > C20, but no activity on C18 and C16 or fewer carbons
724655
Cryptosporidium parvum
fatty aldehyde + CoA + NAD(P)+
since aldehydes are toxic to cells it is very likely that the fatty aldehyde is immediately transformed into the corresponding alcohol by the enzyme complex
-
-
?
fatty acyl-[acyl-carrier protein] + NAD(P)H + H+
-
724655
Cryptosporidium parvum
fatty aldehyde + acyl-carrier protein + NAD(P)+
-
-
-
?
hexacosanoyl-CoA + NAD(P)H + H+
-
724655
Cryptosporidium parvum
hexacosanal + CoA + NAD(P)+
-
-
-
?
additional information
because of technical difficulties in preparing the native substrates for CpFAS1-R, which are very long chain fatty acyl-ACPs, long chain and very long chain fatty acyl-CoAs are used as substrates to assay CpFAS1-R activity
724655
Cryptosporidium parvum
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.077
-
hexacosanoyl-CoA
pH 7.2, temperature not specified in the publication
Cryptosporidium parvum
General Information
General Information
Commentary
Organism
metabolism
C-terminal CpFAS1-R enzyme belongs to a multifunctional Type I fatty acid synthase, CpFAS1, with at least 21 enzymatic domains, this megasynthase is predicted to function as a fatty acyl elongase
Cryptosporidium parvum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
C-terminal CpFAS1-R enzyme belongs to a multifunctional Type I fatty acid synthase, CpFAS1, with at least 21 enzymatic domains, this megasynthase is predicted to function as a fatty acyl elongase
Cryptosporidium parvum
Other publictions for EC 1.2.1.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740064
Warui
Efficient delivery of long-cha ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133
Biochemistry
54
1006-1015
2015
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1
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3
2
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3
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6
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1
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2
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1
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2
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1
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1
1
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3
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2
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3
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1
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2
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2
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1
1
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-
724441
Doan
Biochemical characterization o ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1821
1244-1255
2012
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1
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1
1
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8
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1
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6
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2
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1
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1
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2
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1
2
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1
1
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1
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6
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2
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1
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713511
Schirmer
Microbial biosynthesis of alka ...
Synechococcus elongatus, Synechococcus elongatus PCC 7942, Synechococcus sp., Synechococcus sp. PCC 7942
Science
329
559-562
2010
-
-
1
-
1
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1
-
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4
-
36
-
-
-
-
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10
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6
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-
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2
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-
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1
2
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1
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1
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4
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10
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724655
Zhu
The reductase domain in a Type ...
Cryptosporidium parvum
BMC Biochem.
11
46
2010
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1
1
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1
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1
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1
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1
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-
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4
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2
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1
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-
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2
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-
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1
1
2
-
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-
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1
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1
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1
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4
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1
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1
1
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