BRENDA - Enzyme Database
show all sequences of 1.2.1.80

Biochemical characterization of a chloroplast localized fatty acid reductase from Arabidopsis thaliana

Doan, T.T.; Domergue, F.; Fournier, A.E.; Vishwanath, S.J.; Rowland, O.; Moreau, P.; Wood, C.C.; Carlsson, A.S.; Hamberg, M.; Hofvander, P.; Biochim. Biophys. Acta 1821, 1244-1255 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence expressed in Escherichia coli, both enzymes fused at the C-terminus to a His Tag containing maltose binding protein; AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence subcloned into the pYES-DEST52 plasmid downstream of the GAL1 promoter and transformed into Saccharomyces cerevisiae W303-1A strain
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00148
-
hexadecanoyl-[acyl-carrier protein]
pH 7.0, at 30°C
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
transient expression of fusion proteins of AtFAR6 and yellow fluorescent protein in Nicotiana sp. leaves show that AtFAR6 is chloroplast-localized
Arabidopsis thaliana
9507
-
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
in Escherichia coli expressed AtFAR6 and mAtFAR6 purified by a HisTrap HP column BioLogic LP liquid chromatography system
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
AtFAR6 promoter active in epidermis, endothecium and tapetum of anthers, histochemical staining of AtFAR6 promoter-GUS reporter gene activity
Arabidopsis thaliana
-
additional information
no AtFAR6 promoter-GUS reporter gene activity in the inner cortex, vascular bundles or flower microspores
Arabidopsis thaliana
-
plant epidermis
AtFAR6 promoter active in stem layer, histochemical staining of AtFAR6 promoter-GUS reporter gene activity
Arabidopsis thaliana
-
receptacle
AtFAR6 promoter active in the replum and receptacle of siliques
Arabidopsis thaliana
-
root cap
throughout lateral and primary root development, histochemical staining of AtFAR6 promoter-GUS reporter gene activity
Arabidopsis thaliana
-
root primordium
histochemical staining of AtFAR6 promoter-GUS reporter gene activity
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
acyl-CoA and acyl-ACP can serve as substrates
724441
Arabidopsis thaliana
hexadecanal + acyl-carrier protein + NAD(P)+
hexadecanal is a reaction intermediate that is further converted into hexadecanol
-
-
?
additional information
in Saccharomyces cerevisiae overexpressed truncated version of AtFAR6, which lacks the N-terminal extension, produces C16:0 primary fatty alcohol as well as C18:0 fatty alcohols much more efficiently than the full length protein
724441
Arabidopsis thaliana
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
AtFAR6
-
Arabidopsis thaliana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
10
truncated mAtFAR6 protein
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no significant activity of truncated mAtFAR6 observed when NADH is used as cofactor
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence expressed in Escherichia coli, both enzymes fused at the C-terminus to a His Tag containing maltose binding protein; AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence subcloned into the pYES-DEST52 plasmid downstream of the GAL1 promoter and transformed into Saccharomyces cerevisiae W303-1A strain
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no significant activity of truncated mAtFAR6 observed when NADH is used as cofactor
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00148
-
hexadecanoyl-[acyl-carrier protein]
pH 7.0, at 30°C
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
transient expression of fusion proteins of AtFAR6 and yellow fluorescent protein in Nicotiana sp. leaves show that AtFAR6 is chloroplast-localized
Arabidopsis thaliana
9507
-
Purification (Commentary) (protein specific)
Commentary
Organism
in Escherichia coli expressed AtFAR6 and mAtFAR6 purified by a HisTrap HP column BioLogic LP liquid chromatography system
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
AtFAR6 promoter active in epidermis, endothecium and tapetum of anthers, histochemical staining of AtFAR6 promoter-GUS reporter gene activity
Arabidopsis thaliana
-
additional information
no AtFAR6 promoter-GUS reporter gene activity in the inner cortex, vascular bundles or flower microspores
Arabidopsis thaliana
-
plant epidermis
AtFAR6 promoter active in stem layer, histochemical staining of AtFAR6 promoter-GUS reporter gene activity
Arabidopsis thaliana
-
receptacle
AtFAR6 promoter active in the replum and receptacle of siliques
Arabidopsis thaliana
-
root cap
throughout lateral and primary root development, histochemical staining of AtFAR6 promoter-GUS reporter gene activity
Arabidopsis thaliana
-
root primordium
histochemical staining of AtFAR6 promoter-GUS reporter gene activity
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
acyl-CoA and acyl-ACP can serve as substrates
724441
Arabidopsis thaliana
hexadecanal + acyl-carrier protein + NAD(P)+
hexadecanal is a reaction intermediate that is further converted into hexadecanol
-
-
?
additional information
in Saccharomyces cerevisiae overexpressed truncated version of AtFAR6, which lacks the N-terminal extension, produces C16:0 primary fatty alcohol as well as C18:0 fatty alcohols much more efficiently than the full length protein
724441
Arabidopsis thaliana
?
-
-
-
?
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
10
truncated mAtFAR6 protein
Arabidopsis thaliana
Other publictions for EC 1.2.1.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740064
Warui
Efficient delivery of long-cha ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133
Biochemistry
54
1006-1015
2015
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1
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3
2
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3
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6
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1
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2
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1
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2
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1
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1
1
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3
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2
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3
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1
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2
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2
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-
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-
-
1
1
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-
724441
Doan
Biochemical characterization o ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1821
1244-1255
2012
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1
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1
1
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8
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1
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6
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2
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1
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1
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2
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1
2
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1
1
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1
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6
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2
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1
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713511
Schirmer
Microbial biosynthesis of alka ...
Synechococcus elongatus, Synechococcus elongatus PCC 7942, Synechococcus sp., Synechococcus sp. PCC 7942
Science
329
559-562
2010
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1
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1
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1
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4
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36
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10
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6
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2
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1
2
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1
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1
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4
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10
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724655
Zhu
The reductase domain in a Type ...
Cryptosporidium parvum
BMC Biochem.
11
46
2010
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1
1
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1
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1
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1
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1
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4
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2
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1
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2
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1
1
2
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1
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1
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1
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4
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1
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1
1
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