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Literature summary for 1.2.1.80 extracted from

  • Doan, T.T.; Domergue, F.; Fournier, A.E.; Vishwanath, S.J.; Rowland, O.; Moreau, P.; Wood, C.C.; Carlsson, A.S.; Hamberg, M.; Hofvander, P.
    Biochemical characterization of a chloroplast localized fatty acid reductase from Arabidopsis thaliana (2012), Biochim. Biophys. Acta, 1821, 1244-1255.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence expressed in Escherichia coli, both enzymes fused at the C-terminus to a His Tag containing maltose binding protein Arabidopsis thaliana
AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence subcloned into the pYES-DEST52 plasmid downstream of the GAL1 promoter and transformed into Saccharomyces cerevisiae W303-1A strain Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00148
-
hexadecanoyl-[acyl-carrier protein] pH 7.0, at 30°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast transient expression of fusion proteins of AtFAR6 and yellow fluorescent protein in Nicotiana sp. leaves show that AtFAR6 is chloroplast-localized Arabidopsis thaliana 9507
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
in Escherichia coli expressed AtFAR6 and mAtFAR6 purified by a HisTrap HP column BioLogic LP liquid chromatography system Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower AtFAR6 promoter active in epidermis, endothecium and tapetum of anthers, histochemical staining of AtFAR6 promoter-GUS reporter gene activity Arabidopsis thaliana
-
additional information no AtFAR6 promoter-GUS reporter gene activity in the inner cortex, vascular bundles or flower microspores Arabidopsis thaliana
-
plant epidermis AtFAR6 promoter active in stem layer, histochemical staining of AtFAR6 promoter-GUS reporter gene activity Arabidopsis thaliana
-
receptacle AtFAR6 promoter active in the replum and receptacle of siliques Arabidopsis thaliana
-
root cap throughout lateral and primary root development, histochemical staining of AtFAR6 promoter-GUS reporter gene activity Arabidopsis thaliana
-
root primordium histochemical staining of AtFAR6 promoter-GUS reporter gene activity Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+ acyl-CoA and acyl-ACP can serve as substrates Arabidopsis thaliana hexadecanal + acyl-carrier protein + NAD(P)+ hexadecanal is a reaction intermediate that is further converted into hexadecanol ?
additional information in Saccharomyces cerevisiae overexpressed truncated version of AtFAR6, which lacks the N-terminal extension, produces C16:0 primary fatty alcohol as well as C18:0 fatty alcohols much more efficiently than the full length protein Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
AtFAR6
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
4 10 truncated mAtFAR6 protein Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
additional information no significant activity of truncated mAtFAR6 observed when NADH is used as cofactor Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana