BRENDA - Enzyme Database
show all sequences of 1.2.1.80

Microbial biosynthesis of alkanes

Schirmer, A.; Rude, M.A.; Li, X.; Popova, E.; del Cardayre, S.B.; Science 329, 559-562 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of strain PCC7942 orf1593 and orf1594, the latter encoding an aldehde decarbonylase, both separately and together in Escherichia coli. Extracts of recombinant PCC7942_orf1594-expressing cells contain substantial quantities of evenchain fatty aldehydes and fatty alcohols, whereas coexpression of both PCC7942_orf1593 and orf1594 results in the production of odd chain alkanes and alkenes, as well as even-chain fatty aldehydes and fatty alcohols. Orf1593 and orf1594 appear to be part of a larger operon that contains a gene encoding a subunit of the acetyl-CoA carboxylase, accA, which is essential for growth
Synechococcus sp.
Engineering
Amino acid exchange
Commentary
Organism
additional information
replacement of the PC7942_orf1593 and 1594 orthologues in Synechocystis sp. PCC6803, where the orthologous genes apparently form only a bicistronic operon, with a kanamycin-resistance cassette. This replacement abolishes the presence of alkanes in the extracts of photoautoptrophically grown cells. PCC7942_orf 1593 and orf1594 coexpression in an Escherichia coli strain devoid of acyl-CoAs leads to very similar levels of hydrocarbon production, comparable to those in an Escherichia coli wild-type strain
Synechococcus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0079
-
oleoyl-[acyl carrier protein]
pH and temperature not specified in the publication
Synechococcus elongatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fatty acyl-[acyl carrier protein] + NAD(P)H + H+
Synechococcus elongatus
kinetically preferred and likely the in vivo substrate
fatty aldehyde + acyl carrier protein + NAD(P)+
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
Synechococcus sp.
acyl-ACP is kinetically preferred and is likely the in vivo substrate
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
Synechococcus sp. PCC 7942
acyl-ACP is kinetically preferred and is likely the in vivo substrate
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Synechococcus elongatus
-
-
-
Synechococcus sp.
-
orf1594 or sll0209
-
Synechococcus sp. PCC 7942
-
orf1594 or sll0209
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fatty acyl-[acyl carrier protein] + NAD(P)H + H+
kinetically preferred and likely the in vivo substrate
713511
Synechococcus elongatus
fatty aldehyde + acyl carrier protein + NAD(P)+
-
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
substrate selectivity
713511
Synechococcus sp.
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
acyl-ACP is kinetically preferred and is likely the in vivo substrate
713511
Synechococcus sp.
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
substrate selectivity
713511
Synechococcus sp. PCC 7942
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
acyl-ACP is kinetically preferred and is likely the in vivo substrate
713511
Synechococcus sp. PCC 7942
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
-
?
additional information
does not reduce acyl-CoA
713511
Synechococcus elongatus
?
-
-
-
-
oleoyl-[acyl carrier protein] + NAD(P)H + H+
-
713511
Synechococcus elongatus
oleic aldehyde + acyl carrier protein + NAD(P)+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Synechococcus elongatus
NAD(P)H
-
Synechococcus sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of strain PCC7942 orf1593 and orf1594, the latter encoding an aldehde decarbonylase, both separately and together in Escherichia coli. Extracts of recombinant PCC7942_orf1594-expressing cells contain substantial quantities of evenchain fatty aldehydes and fatty alcohols, whereas coexpression of both PCC7942_orf1593 and orf1594 results in the production of odd chain alkanes and alkenes, as well as even-chain fatty aldehydes and fatty alcohols. Orf1593 and orf1594 appear to be part of a larger operon that contains a gene encoding a subunit of the acetyl-CoA carboxylase, accA, which is essential for growth
Synechococcus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Synechococcus elongatus
NAD(P)H
-
Synechococcus sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
replacement of the PC7942_orf1593 and 1594 orthologues in Synechocystis sp. PCC6803, where the orthologous genes apparently form only a bicistronic operon, with a kanamycin-resistance cassette. This replacement abolishes the presence of alkanes in the extracts of photoautoptrophically grown cells. PCC7942_orf 1593 and orf1594 coexpression in an Escherichia coli strain devoid of acyl-CoAs leads to very similar levels of hydrocarbon production, comparable to those in an Escherichia coli wild-type strain
Synechococcus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0079
-
oleoyl-[acyl carrier protein]
pH and temperature not specified in the publication
Synechococcus elongatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fatty acyl-[acyl carrier protein] + NAD(P)H + H+
Synechococcus elongatus
kinetically preferred and likely the in vivo substrate
fatty aldehyde + acyl carrier protein + NAD(P)+
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
Synechococcus sp.
acyl-ACP is kinetically preferred and is likely the in vivo substrate
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
Synechococcus sp. PCC 7942
acyl-ACP is kinetically preferred and is likely the in vivo substrate
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fatty acyl-[acyl carrier protein] + NAD(P)H + H+
kinetically preferred and likely the in vivo substrate
713511
Synechococcus elongatus
fatty aldehyde + acyl carrier protein + NAD(P)+
-
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
substrate selectivity
713511
Synechococcus sp.
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
acyl-ACP is kinetically preferred and is likely the in vivo substrate
713511
Synechococcus sp.
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
substrate selectivity
713511
Synechococcus sp. PCC 7942
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
-
?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+
acyl-ACP is kinetically preferred and is likely the in vivo substrate
713511
Synechococcus sp. PCC 7942
hexadecanal + acyl-carrier protein + NAD(P)+
-
-
-
?
additional information
does not reduce acyl-CoA
713511
Synechococcus elongatus
?
-
-
-
-
oleoyl-[acyl carrier protein] + NAD(P)H + H+
-
713511
Synechococcus elongatus
oleic aldehyde + acyl carrier protein + NAD(P)+
-
-
-
?
Other publictions for EC 1.2.1.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740064
Warui
Efficient delivery of long-cha ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133
Biochemistry
54
1006-1015
2015
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-
1
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3
2
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3
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6
-
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1
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2
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2
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1
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1
1
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3
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2
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3
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1
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2
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2
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1
1
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-
724441
Doan
Biochemical characterization o ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1821
1244-1255
2012
-
-
1
-
-
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1
1
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8
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1
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6
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2
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1
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2
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1
2
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1
1
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1
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6
-
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2
-
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-
-
-
-
1
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-
-
-
-
-
-
-
713511
Schirmer
Microbial biosynthesis of alka ...
Synechococcus elongatus, Synechococcus sp., Synechococcus sp. PCC 7942
Science
329
559-562
2010
-
-
1
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1
-
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1
-
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3
-
22
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-
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7
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2
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1
2
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1
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1
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3
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7
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724655
Zhu
The reductase domain in a Type ...
Cryptosporidium parvum
BMC Biochem.
11
46
2010
-
1
1
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1
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1
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1
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1
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4
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1
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2
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1
1
2
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1
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1
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1
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4
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1
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1
1
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