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Literature summary for 1.2.1.8 extracted from

  • Bhatt, V.; Barvkar, V.T.; Furtado, A.; Henry, R.J.; Nadaf, A.
    Fragrance in Pandanus amaryllifolius Roxb. despite the presence of a betaine aldehyde dehydrogenase 2 (2021), Int. J. Mol. Sci., 22, 6968 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene BADH2, reconstructed from transcriptome, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR enzyme expression analysis Pandanus amaryllifolius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Pandanus amaryllifolius
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betaine + NADH + 2 H+
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?

Organism

Organism UniProt Comment Textmining
Pandanus amaryllifolius A0A2Z2GYT8
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-

Source Tissue

Source Tissue Comment Organism Textmining
leaf the site of storage for aroma volatile 2-acetyl-1-pyrroline (2AP) are bag-like structures called epidermal papillae identified on the lower epidermis of the leaves of the species Pandanus amaryllifolius
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seedling
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Pandanus amaryllifolius
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
quantification of 2-acetyl-1-pyrroline (2AP) and other metabolites in vivo Pandanus amaryllifolius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutyraldehyde + NAD+ + H2O
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Pandanus amaryllifolius 4-aminobutyrate + NADH + 2 H+
-
?
betaine aldehyde + NAD+ + H2O
-
Pandanus amaryllifolius betaine + NADH + 2 H+
-
?
additional information betaine aldehyde substrates docking study, overview. The enzyme shows a good affinity with both betaine aldehyde (BAD) and 4-aminobutyraldehyde (GABald) as substrates Pandanus amaryllifolius ?
-
-

Subunits

Subunits Comment Organism
? x * 54000, about, sequence calculation Pandanus amaryllifolius

Synonyms

Synonyms Comment Organism
BADH2
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Pandanus amaryllifolius
betaine aldehyde dehydrogenase 2
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Pandanus amaryllifolius
PaBADH2
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Pandanus amaryllifolius
PND BADH2
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Pandanus amaryllifolius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pandanus amaryllifolius

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pandanus amaryllifolius

General Information

General Information Comment Organism
additional information three-dimensional docking analysis of PaBADH2 with betaine aldehyde Pandanus amaryllifolius
physiological function Pandanus amaryllifolius accumulates the highest concentration of the major basmati aroma volatile 2-acetyl-1-pyrroline (2AP) in the plant kingdom. The expression of 2AP is correlated with the presence of a nonfunctional betaine aldehyde dehydrogenase 2 (BADH2) in aromatic rice and other plant species. 2AP biosynthesis in Pandanus amaryllifolius is not due to the inactivation of BADH2 Pandanus amaryllifolius