Application | Comment | Organism |
---|---|---|
biotechnology | BADH overexpression in maize is beneficial for drought tolerance and the three transgenic maize lines can be used for further breeding experiments. The agronomic traits of transgenic maize are not affected by the overexpression of BADH | Atriplex centralasiatica |
Cloned (Comment) | Organism |
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gene BADH, phylogenetic analysis, recombinant expression in Zea mays, quantitative RT-PCR expression analysis | Atriplex centralasiatica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | glycine betaine (GB) accumulation in transgenic crops following heterologous overexpression of the BADH gene dramatically improves the tolerance to salt, cold, and oxidative stresses. The root system of transgenic maize is more developed than that of wild-type maize, and the data showed the dry root weight of transgenic maize increased compared with the wild-type. The drought tolerance of high-generation BADH transgenic maize inbred lines at different growth stages using the hyperosmotic solution and water-withholding methods are analyzed. Molecular detection reveals that exogenous BADH is successfully introduced into the maize plant genome and overexpressed in three transgenic maize inbred lines. Under osmotic stress, transgenic maize hold better germination ability than the unmodified Dan988 wild-type line. In addition, transgenic maize contain higher levels of antioxidant enzymes and osmotic regulatory substances compared with wild-type, and thus accumulate less harmful substances and this alleviates the negative effects of drought. Engineered line Dan988-BADH-4 shows the best tolerance, followed by Dan988-BADH-2 and Dan988-BADH-1 while wild-type ranks lowest. BADH overexpression in maize is beneficial for drought tolerance. The agronomic traits of transgenic maize are not affected by the overexpression of BADH | Atriplex centralasiatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | Atriplex centralasiatica | - |
betaine + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Atriplex centralasiatica | Q8L8I1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | - |
Atriplex centralasiatica | betaine + NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AcBADH | - |
Atriplex centralasiatica |
BADH | - |
Atriplex centralasiatica |
betaine aldehyde dehydrogenase | - |
Atriplex centralasiatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Atriplex centralasiatica |
General Information | Comment | Organism |
---|---|---|
physiological function | betaine aldehyde dehydrogenase (BADH) is a key enzyme in glycine betaine (GB) synthesis, and the activity of the enzyme is significantly increased when plants are under abiotic stress, thus greatly increasing the accumulation of glycine betaine | Atriplex centralasiatica |