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Literature summary for 1.2.1.8 extracted from

  • Munoz-Clares, R.A.; Riveros-Rosas, H.; Garza-Ramos, G.; Gonzalez-Segura, L.; Mujica-Jimenez, C.; Julian-Sanchez, A.
    Exploring the evolutionary route of the acquisition of betaine aldehyde dehydrogenase activity by plant ALDH10 enzymes: implications for the synthesis of the osmoprotectant glycine betaine (2014), BMC Plant Biol., 14, 149.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.8

Protein Variants

Protein Variants Comment Organism
A441C the mutant exhibited almost wild type activity with betaine aldehyde Spinacia oleracea
A441F the mutant shows no activity with betaine aldehyde Spinacia oleracea
A441I the mutant shows no activity with betaine aldehyde Spinacia oleracea
A441S the mutant exhibits slightly reduced activity with betaine aldehyde Spinacia oleracea
A441T the mutant exhibits clearly reduced activity with betaine aldehyde Spinacia oleracea
A441V the mutant shows no activity with betaine aldehyde Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 NAD+ mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea
0.0064
-
 NAD+ mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
0.014
-
 NAD+ mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
0.018
-
 NAD+ mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
0.022
-
 NAD+ wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea
0.024
-
 NAD+ mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
0.029
-
 NAD+ mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
0.09
-
 Betaine aldehyde mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
0.098
-
 Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea
0.119
-
 Betaine aldehyde mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
0.18
-
 Betaine aldehyde mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
0.512
-
 Betaine aldehyde mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
0.605
-
 Betaine aldehyde mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
1.791
-
 Betaine aldehyde mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Spinacia oleracea
-
 betaine + NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea P17202
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD+ + H2O
-
 Spinacia oleracea betaine + NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
ALD10
-
 Spinacia oleracea
BADH
-
 Spinacia oleracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 Betaine aldehyde mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
0.39
-
 NAD+ mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
0.51
-
 NAD+ mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
0.54
-
 Betaine aldehyde mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
0.68
-
 NAD+ mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea
0.74
-
 Betaine aldehyde mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea
2.2
-
 NAD+ mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
2.39
-
 NAD+ mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
2.64
-
 Betaine aldehyde mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
2.99
-
 Betaine aldehyde mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
3.27
-
 NAD+ mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
3.29
-
 Betaine aldehyde mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
3.36
-
 Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea
4.25
-
 NAD+ wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Spinacia oleracea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.41
-
 Betaine aldehyde mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea
0.48
-
 Betaine aldehyde mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
1.1
-
 Betaine aldehyde mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
15
-
 Betaine aldehyde mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
22
-
 NAD+ mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
28
-
 Betaine aldehyde mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
33
-
 Betaine aldehyde mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
35
-
 Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea
80
-
 NAD+ mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
100
-
 NAD+ mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
114
-
 NAD+ mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
179
-
 NAD+ mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
195
-
 NAD+ wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea
243
-
 NAD+ mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea