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Literature summary for 1.2.1.8 extracted from
- Site-directed mutagenesis and homology modeling indicate an important role of cysteine 439 in the stability of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa (2005), Biochimie, 87, 1056-1064.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | betaine aldehyde dehydrogenase is a potential target for antimicrobial agents against Pseudomonas aeruginosa | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C353A | steady-state kinetic and structure not significantly affected | Pseudomonas aeruginosa |
| C377A | steady-state kinetic and structure not significantly affected | Pseudomonas aeruginosa |
| C439A | steady-state kinetic and structure not significantly affected, stability severely reduced | Pseudomonas aeruginosa |
| C439S | steady-state kinetic and structure not significantly affected, stability severely reduced | Pseudomonas aeruginosa |
| C439V | steady-state kinetic and structure not significantly affected, stability severely reduced | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | for the mutants and wild-type enzymes KM NADP+ varies within 0.060 and 0.107 mM, KM NAD+ within 0.254 and 0.411 mM, KM betaine aldehyde within 0.270 and 0.434 mM | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| betaine aldehyde + NAD(P)+ + H2O | Pseudomonas aeruginosa | - |
glycine betaine + NAD(P)H + 2 H+ | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified to homogeneity following a two-step procedure, proteins expressed in Escherichia coli | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 75 | - |
mutant C439V | Pseudomonas aeruginosa |
| 102 | - |
mutant C439S | Pseudomonas aeruginosa |
| 138 | - |
mutant C353A | Pseudomonas aeruginosa |
| 165 | - |
mutant C377A | Pseudomonas aeruginosa |
| 189 | - |
mutant C439A | Pseudomonas aeruginosa |
| 196 | - |
wild-type enzyme | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| betaine aldehyde + NAD(P)+ + H2O | - |
Pseudomonas aeruginosa | glycine betaine + NAD(P)H + 2 H+ | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BADH | - |
Pseudomonas aeruginosa |
| betaine aldehyde dehydrogenase | - |
Pseudomonas aeruginosa |
| betaine aldehyde:NAD+ oxidoreductase | - |
Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 34.3 | - |
apparent T50 mutant C439V | Pseudomonas aeruginosa |
| 37 | - |
apparent T50 mutant C439S | Pseudomonas aeruginosa |
| 43.6 | - |
apparent T50 mutant C439A | Pseudomonas aeruginosa |
| 46 | - |
apparent T50 mutant C377A | Pseudomonas aeruginosa |
| 49.4 | - |
apparent T50 mutant C353A | Pseudomonas aeruginosa |
| 50.7 | - |
apparent T50 wild-type enzyme | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD(P)+ | - |
Pseudomonas aeruginosa | |
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