Literature summary for 1.2.1.8 extracted from

Velasco-Garcia, R.; Villalobos, M.A.; Ramirez-Romero, M.A.; Mujica-Jimenez, C.; Iturriaga, G.; Munoz-Clares, R.A.
Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa: cloning, over-expression in Escherichia coli, and regulation by choline and salt (2006), Arch. Microbiol., 185, 14-22.

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Application

Application	Comment	Organism
medicine	Pseudomonas aeruginosa is an important pathogen, glycine betaine could play an important physiological role under the conditions present in the infection sites	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
the gene betB, encoding for Pseudomonas aeruginosa betaine-aldehyde dehydrogenase, is cloned into a pCAL-n vector for sequencing and protein expression in Escherichia coli	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53330	-	monomer, amino acid sequence	Pseudomonas aeruginosa
61000	-	SDS-PAGE, Coomassie blue staining and Western blot	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
betaine aldehyde + NAD+ + H2O	Pseudomonas aeruginosa	-	glycine betaine + NADH + 2 H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9HTJ1	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified on Q-Sepharose fast flow and 2', 5'-ADP-Sepharose columns, 28 mg of pure protein per liter of culture are yielded	Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.006	-	culture conditions, 11.1 mM glucose and 15 mM NH4Cl	Pseudomonas aeruginosa
0.012	-	culture conditions, 11.1 mM glucose, 15 mM NH4Cl and 0.4 M NaCl	Pseudomonas aeruginosa
0.211	-	culture conditions, 11.1 mM glucose, 20 mM choline and 0.4 M NaCl	Pseudomonas aeruginosa
0.215	-	culture conditions, 11.1 mM glucose and 20 mM choline	Pseudomonas aeruginosa
0.492	-	culture conditions, 20 mM choline and 0.4 M NaCl	Pseudomonas aeruginosa
0.571	-	culture conditions, 20 mM choline	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
betaine aldehyde + NAD+ + H2O	-	Pseudomonas aeruginosa	glycine betaine + NADH + 2 H+	-	r

Subunits

Subunits	Comment	Organism
tetramer	4 x 61000, SDS-PAGE	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
BADH	-	Pseudomonas aeruginosa
betaine aldehyde:NAD(P)+ oxidoreductase	-	Pseudomonas aeruginosa
betaine-aldehyde dehydrogenase	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	activity assay	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	activity assay	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)