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Literature summary for 1.2.1.8 extracted from

  • Falkenberg, P.; Strom, A.R.
    Purification and characterization of osmoregulatory betaine aldehyde dehydrogenase of Escherichia coli (1990), Biochim. Biophys. Acta, 1034, 253-259.
    View publication on PubMed
Search for more literature for 1.2.1.8

General Stability

General Stability Organism
salt-tolerant enzyme retains 50% of its initial activity at 1.2 M K+ Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde 100 mM, 83% inhibition Escherichia coli
benzaldehyde 1 mM, 51% inhibition Escherichia coli
Betaine aldehyde above 0.5 mM Escherichia coli
Butyrylcholine 100 mM, 73% inhibition Escherichia coli
choline 100 mM, 78% inhibition Escherichia coli
ethanolamine 100 mM, 35% inhibition Escherichia coli
glyceraldehyde 100 mM, 83% inhibition Escherichia coli
Hg2+ 0.0001-0.0005 mM HgCl2 Escherichia coli
iodoacetamide 1 mM Escherichia coli
iodoacetate 1 mM Escherichia coli
isopentanal 1 mM, 84% inhibition Escherichia coli
additional information no substrate inhibition with NAD+ Escherichia coli
N,N-dimethylethanolamine 100 mM, 57% inhibition Escherichia coli
N,N-dimethylglycine 1 mM, 24% inhibition Escherichia coli
N-Methylethanolamine 100 mM, 50% inhibition Escherichia coli
N-methylglycine 1 mM, 24% inhibition Escherichia coli
NADP+ substrate inhibition above 10 mM Escherichia coli
PCMB 0.0001-0.0005 mM Escherichia coli
phenylacetaldehyde 1 mM, 54% inhibition Escherichia coli
tetramethylammonium hydroxide 100 mM, 57% inhibition Escherichia coli
trimethylacetaldehyde 100 mM, 89% inhibition Escherichia coli
ZnCl2 0.0001-0.0005 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.099
-
 NAD+
-
 Escherichia coli
0.16
-
 Betaine aldehyde
-
 Escherichia coli
0.4
-
 NADP+
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activation up to 0.15 M, decreases activity at higher concentrations Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 4 * 55000, SDS-PAGE Escherichia coli
232000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Escherichia coli the salt tolerance is an essential property for the enzyme participating in the cellular synthesis of an osmoprotectant betaine + NADH
-
 ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 over-producing strain carrying the structural gene for the enzyme on the plasmid pBR322
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-80°C, frozen in presence of 10 mM dithiothreitol, stable for several months without appreciable loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD+ + H2O highly specific for betaine aldehyde Escherichia coli betaine + NADH
-
 ir
betaine aldehyde + NAD+ + H2O the salt tolerance is an essential property for the enzyme participating in the cellular synthesis of an osmoprotectant Escherichia coli betaine + NADH
-
 ir
betaine aldehyde + NADP+ + H2O NAD+ is preferred over NADP+ Escherichia coli betaine + NADPH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ is preferred over NADP+ Escherichia coli
NADP+ NAD+ is preferred over NADP+ Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
 isopentanal
-
 Escherichia coli
0.53
-
 phenylacetaldehyde
-
 Escherichia coli
0.7
-
 benzaladehyde
-
 Escherichia coli
4.5
-
 trimethylacetaldehyde
-
 Escherichia coli
5.7
-
 acetaldehyde
-
 Escherichia coli
7.4
-
 glyceraldehyde
-
 Escherichia coli
11
-
 choline
-
 Escherichia coli
14
-
 Butyrylcholine
-
 Escherichia coli
26
-
 tetramethylammonium hydroxide
-
 Escherichia coli
27
-
 N,N-dimethylethanolamine
-
 Escherichia coli
40
-
 N-Methylethanolamine
-
 Escherichia coli
78
-
 ethanolamine
-
 Escherichia coli
130
-
 N,N-dimethylglycine
-
 Escherichia coli
130
-
 N-methylglycine
-
 Escherichia coli