BRENDA - Enzyme Database
show all sequences of 1.2.1.77

Aerobic benzoyl-coenzyme A (CoA) catabolic pathway in: conversion of ring cleavage product by 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase

Gescher, J.; Ismail, W.; Olgeschläger, E.; Eisenreich, W.; Wörth, J.; Fuchs, G.; J. Bacteriol. 188, 2919-2927 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli as a protein tagged at its N terminus with maltose-binding protein
Aromatoleum evansii
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
salt concentrations up to 500 mM KCl and EDTA concentrations up to 50 mM have no effect on enzyme activity
Aromatoleum evansii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
NADP+
pH 7.2
Aromatoleum evansii
0.025
-
3,4-dehydroadipyl-CoA semialdehyde
pH 7.2
Aromatoleum evansii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
salt concentrations up to 500 mM KCl have no effect on enzyme activity
Aromatoleum evansii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
2 * 54000
Aromatoleum evansii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
Aromatoleum evansii
enzyme of the aerobic benzoyl-coenzyme A catabolic pathway
3,4-dehydroadipyl-CoA + NADPH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aromatoleum evansii
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Aromatoleum evansii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.17
-
extracts of cells grown aerobically on benzoate
Aromatoleum evansii
Storage Stability
Storage Stability
Organism
-20°C, enzyme could be stored without appreciable loss of activity for months at -20°C in the presence of 10% (v/v) glycerol
Aromatoleum evansii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
enzyme of the aerobic benzoyl-coenzyme A catabolic pathway
698572
Aromatoleum evansii
3,4-dehydroadipyl-CoA + NADPH + H+
-
-
-
?
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
the molar ratio of mol of converted substrate per mol of NADPH formed is estimated to be 1
698572
Aromatoleum evansii
3,4-dehydroadipyl-CoA + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 54000
Aromatoleum evansii
Synonyms
Synonyms
Commentary
Organism
3,4-dehydroadipyl-CoA semialdehyde dehydrogenase
-
Aromatoleum evansii
BoxD
-
Aromatoleum evansii
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
45
-
NADP+
pH 7.2
Aromatoleum evansii
45
-
3,4-dehydroadipyl-CoA semialdehyde
pH 7.2
Aromatoleum evansii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Aromatoleum evansii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.2
8.8
50% of maximal activity at pH 6.2 and pH 8.8
Aromatoleum evansii
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
no activity (below 2%) with NAD+. The molar ratio of mol of converted substrate per mol of NADPH formed is estimated to be 1
Aromatoleum evansii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli as a protein tagged at its N terminus with maltose-binding protein
Aromatoleum evansii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
no activity (below 2%) with NAD+. The molar ratio of mol of converted substrate per mol of NADPH formed is estimated to be 1
Aromatoleum evansii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
salt concentrations up to 500 mM KCl and EDTA concentrations up to 50 mM have no effect on enzyme activity
Aromatoleum evansii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
NADP+
pH 7.2
Aromatoleum evansii
0.025
-
3,4-dehydroadipyl-CoA semialdehyde
pH 7.2
Aromatoleum evansii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
salt concentrations up to 500 mM KCl have no effect on enzyme activity
Aromatoleum evansii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
2 * 54000
Aromatoleum evansii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
Aromatoleum evansii
enzyme of the aerobic benzoyl-coenzyme A catabolic pathway
3,4-dehydroadipyl-CoA + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aromatoleum evansii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.17
-
extracts of cells grown aerobically on benzoate
Aromatoleum evansii
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, enzyme could be stored without appreciable loss of activity for months at -20°C in the presence of 10% (v/v) glycerol
Aromatoleum evansii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
enzyme of the aerobic benzoyl-coenzyme A catabolic pathway
698572
Aromatoleum evansii
3,4-dehydroadipyl-CoA + NADPH + H+
-
-
-
?
3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
the molar ratio of mol of converted substrate per mol of NADPH formed is estimated to be 1
698572
Aromatoleum evansii
3,4-dehydroadipyl-CoA + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 54000
Aromatoleum evansii
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
45
-
NADP+
pH 7.2
Aromatoleum evansii
45
-
3,4-dehydroadipyl-CoA semialdehyde
pH 7.2
Aromatoleum evansii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Aromatoleum evansii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.2
8.8
50% of maximal activity at pH 6.2 and pH 8.8
Aromatoleum evansii
Other publictions for EC 1.2.1.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726445
Bains
Elucidating the reaction mecha ...
Paraburkholderia xenovorans
Protein Sci.
20
1048-1059
2011
-
-
1
1
8
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
7
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
7
-
-
-
-
-
1
1
-
-
-
712441
Rather
Coenzyme A-dependent aerobic m ...
Aromatoleum evansii, Aromatoleum evansii KB740
J. Biol. Chem.
285
20615-20624
2010
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688414
Bajns J.
Structural and biochemical cha ...
Paraburkholderia xenovorans
J. Mol. Biol.
379
597-608
2008
-
-
1
1
-
-
-
8
-
-
-
1
-
1
-
-
1
-
-
-
-
-
9
1
1
-
-
-
8
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
8
-
-
-
1
-
-
-
1
-
-
-
-
9
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
698572
Gescher
Aerobic benzoyl-coenzyme A (Co ...
Aromatoleum evansii
J. Bacteriol.
188
2919-2927
2006
-
-
1
-
-
-
1
2
-
1
1
1
-
5
-
-
1
-
-
-
1
1
2
1
2
-
-
-
2
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
2
-
1
1
1
-
-
-
1
-
-
1
1
2
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-