Cloned (Comment) | Organism |
---|---|
gene mcr, separate recombinant expression of the N- and C-terminal subdomains of the enzyme, MCR-C and MCR-N, in Schizosaccharomyces pombe strain FY12804/NBRP, coexpression of acetyl-CoA synthase, gene acsSE, and aldehyde dehydrogenase, gene atd1, under the control of the cam1 promoter | Chloroflexus aurantiacus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe. 3-HP production from glucose and cellobiose via the malonyl-CoA pathway, the mcr gene, encoding the bifunctional malonyl-CoA reductase of Chloroflexus aurantiacus, is dissected into two functionally distinct fragments, and the activities of the encoded protein are balanced. The MCR-C fragment reduces malonyl-CoA to malonate semialdehyde, while the MCR-N fragment reduces malonate semialdehyde to 3-HP. To increase the cellular supply of malonyl-CoA and acetyl-CoA, genes encoding endogenous aldehyde dehydrogenase, acetyl-CoA synthase from Salmonella enterica, and endogenous pantothenate kinase are introduced. The resulting strain produces 3-HP at 1.0 g/l from a culture starting at a glucose concentration of 50 g/l. We also engineered the sugar supply by displaying beta-glucosidase (BGL) on the yeast cell surface. When grown on 50 g/l cellobiose, the beta-glucosidase-displaying strain consumes cellobiose efficiently and produces 3-HP at 3.5 g/l. Under fed-batch conditions starting from cellobiose, this strain produces 3-HP at up to 11.4 g/l, corresponding to a yield of 11.2% | Chloroflexus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + NADPH + H+ | Chloroflexus aurantiacus | - |
malonate semialdehyde + CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | Q6QQP7 | bifunctional enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + NADPH + H+ | - |
Chloroflexus aurantiacus | malonate semialdehyde + CoA + NADP+ | - |
? | |
additional information | the bifunctional enzyme shows malonate semialdehyde reduction activity, EC 1.1.1.298, and also malonyl-CoA reduction activity. The C-terminal subdomain MCR-C reduces malonyl-CoA to malonate semialdehyde, while the N-terminal subdomain MCR-N reduces malonate semialdehyde to 3-HP | Chloroflexus aurantiacus | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
malonyl-CoA reductase | UniProt | Chloroflexus aurantiacus |
MCR | - |
Chloroflexus aurantiacus |
More | see also EC 1.1.1.298 | Chloroflexus aurantiacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Chloroflexus aurantiacus |