KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0238 | - |
NADPH | isolated C-terminal fragment, pH 7.8, 57°C | Chloroflexus aurantiacus | |
0.0417 | - |
NADPH | pH 7.8, 57°C | Chloroflexus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | Q6QQP7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + NADPH + H+ | - |
Chloroflexus aurantiacus | malonate semialdehyde + CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MCR | - |
Chloroflexus aurantiacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.81 | - |
NADPH | pH 7.8, 57°C | Chloroflexus aurantiacus | |
18.6 | - |
NADPH | isolated C-terminal fragment, pH 7.8, 57°C | Chloroflexus aurantiacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Chloroflexus aurantiacus |
General Information | Comment | Organism |
---|---|---|
physiological function | the N-terminal region of MCR (MCR-N, amino acids 1-549) and the C-terminal region of MCR (MCR-C, amino acids 550-1219) are functionally distinct. Malonyl-CoA is reduced into free intermediate malonate semialdehyde with NADPH by the MCR-C fragment, and further reduced to 3-hydroxypropionate by the MCR-N fragment, the initial reduction of malonyl-CoA being rate limiting. The TGXXXG(A)X(1-2)G and YXXXK motifs are important for enzyme activities of both MCR-N and MCR-C fragments, and the enzyme activity increases when MCR is separated into two individual fragments. The MCR-C fragment has higher affinity for malonyl-CoA and 4-times higher Kcat/Km value than MCR | Chloroflexus aurantiacus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
187 | - |
NADPH | pH 7.8, 57°C | Chloroflexus aurantiacus | |
782 | - |
NADPH | isolated C-terminal fragment, pH 7.8, 57°C | Chloroflexus aurantiacus |