Application | Comment | Organism |
---|---|---|
biotechnology | the crystallographic data indicate how to construct a bispecific cofactor binding site and to engineer a malonyl-CoA into methylmalonyl-CoA reductase for polyester building block production | Sulfurisphaera tokodaii |
synthesis | MCR is of biotechnological interest for the synthesis of polyester building blocks | Sulfurisphaera tokodaii |
Cloned (Comment) | Organism |
---|---|
Escherichia coli Rosetta 2 cells transformed with pTrc99A-Mcr plasmid harbouring mcr gene from Sulfolobus tokodaii | Sulfurisphaera tokodaii |
Crystallization (Comment) | Organism |
---|---|
malonyl-CoA reductase in the substrate-free state at 2.05 A resolution and in complex with NADP+ at 1.9 A resolution and in complex with CoA at 2.4 A resolution | Sulfurisphaera tokodaii |
sitting drop method, 18°C | Sulfurisphaera tokodaii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the crystallographic data indicate how to construct a bispecific cofactor binding site and to engineer a malonyl-CoA into methylmalonyl-CoA reductase for polyester building block production | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q96YK1 | - |
- |
Sulfurisphaera tokodaii DSM 16993 | Q96YK1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
recombinant enzyme purified by heat precipitation at 85°C and concentration by ultrafiltration, gel filtration chromatography using a Superdex 200 HR 26/60 gel filtration column and Resource phenyl chromatography using a Resource phenyl column | Sulfurisphaera tokodaii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
malonate semialdehyde + CoA + NADP+ = malonyl-CoA + NADPH + H+ | the protein acts as rigid template to press CoA and NADP into similar S-shaped, superimposable forms | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + NADPH + H+ | - |
Sulfurisphaera tokodaii | malonate semialdehyde + CoA + NADP+ | - |
? | |
malonyl-CoA + NADPH + H+ | structural analysis reveals an unexpected reaction cycle in which NADP+ and CoA successively occupy identical binding sites, proposed reaction mechanism | Sulfurisphaera tokodaii | malonate semialdehyde + CoA + NADP+ | - |
? | |
malonyl-CoA + NADPH + H+ | - |
Sulfurisphaera tokodaii DSM 16993 | malonate semialdehyde + CoA + NADP+ | - |
? | |
malonyl-CoA + NADPH + H+ | structural analysis reveals an unexpected reaction cycle in which NADP+ and CoA successively occupy identical binding sites, proposed reaction mechanism | Sulfurisphaera tokodaii DSM 16993 | malonate semialdehyde + CoA + NADP+ | - |
? | |
succinyl-CoA + NADPH + H+ | - |
Sulfurisphaera tokodaii | succinic semialdehyde + CoA + NADP+ | - |
? | |
succinyl-CoA + NADPH + H+ | - |
Sulfurisphaera tokodaii DSM 16993 | succinic semialdehyde + CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | organized as a dimer of two dimers | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
malonyl-CoA reductase | - |
Sulfurisphaera tokodaii |
MCR | - |
Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Sulfurisphaera tokodaii |