Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.5 mM, 85% inhibition | Chloroflexus aurantiacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADPH | 55°C, pH 7.8 | Chloroflexus aurantiacus | |
0.03 | - |
malonyl-CoA | 55°C, pH 7.8 | Chloroflexus aurantiacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulation in decreasing order: Fe2+, Ca2+, Mg2+ | Chloroflexus aurantiacus | |
Fe2+ | stimulation in decreasing order: Fe2+, Ca2+, Mg2+ | Chloroflexus aurantiacus | |
Mg2+ | stimulation in decreasing order: Fe2+, Ca2+, Mg2+ | Chloroflexus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
145000 | - |
2 * 145000, SDS-PAGE | Chloroflexus aurantiacus |
300000 | - |
gel filtration | Chloroflexus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | - |
bifunctional aldehyde dehydrogenase plus alcohol dehydrogenase | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
55°C, pH 7.8 | Chloroflexus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + NADPH + H+ | - |
Chloroflexus aurantiacus | malonate semialdehyde + NADP+ + CoA | - |
? | |
additional information | enzyme additionally catalyzes the second reduction step of malonate semialdehyde + NADPH + H+ to 3-hydroxypropionate + NADP+. Reverse reaction starting with 3-hydroxypropionate does not require CoA and probably stops at malonate semialdehyde. No substrates are acetyl-CoA, propionyl-CoA, succinyl-CoA, or glyoxylate | Chloroflexus aurantiacus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 145000, SDS-PAGE | Chloroflexus aurantiacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
malonyl-CoA | 55°C, pH 7.8 | Chloroflexus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Chloroflexus aurantiacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADH, NAD+ | Chloroflexus aurantiacus | |
NADP+ | - |
Chloroflexus aurantiacus | |
NADPH | - |
Chloroflexus aurantiacus |