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Literature summary for 1.2.1.75 extracted from

  • Huegler, M.; Menendez, C.; Schaegger, H.; Fuchs, G.
    Malonyl-coenzyme A reductase from Chloroflexus aurantiacus, a key enzyme of the 3-hydroxypropionate cycle for autotrophic CO(2) fixation (2002), J. Bacteriol., 184, 2404-2410.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.5 mM, 85% inhibition Chloroflexus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NADPH 55°C, pH 7.8 Chloroflexus aurantiacus
0.03
-
malonyl-CoA 55°C, pH 7.8 Chloroflexus aurantiacus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation in decreasing order: Fe2+, Ca2+, Mg2+ Chloroflexus aurantiacus
Fe2+ stimulation in decreasing order: Fe2+, Ca2+, Mg2+ Chloroflexus aurantiacus
Mg2+ stimulation in decreasing order: Fe2+, Ca2+, Mg2+ Chloroflexus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
2 * 145000, SDS-PAGE Chloroflexus aurantiacus
300000
-
gel filtration Chloroflexus aurantiacus

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus
-
bifunctional aldehyde dehydrogenase plus alcohol dehydrogenase
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
55°C, pH 7.8 Chloroflexus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + NADPH + H+
-
Chloroflexus aurantiacus malonate semialdehyde + NADP+ + CoA
-
?
additional information enzyme additionally catalyzes the second reduction step of malonate semialdehyde + NADPH + H+ to 3-hydroxypropionate + NADP+. Reverse reaction starting with 3-hydroxypropionate does not require CoA and probably stops at malonate semialdehyde. No substrates are acetyl-CoA, propionyl-CoA, succinyl-CoA, or glyoxylate Chloroflexus aurantiacus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 145000, SDS-PAGE Chloroflexus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
50
-
malonyl-CoA 55°C, pH 7.8 Chloroflexus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Chloroflexus aurantiacus

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADH, NAD+ Chloroflexus aurantiacus
NADP+
-
Chloroflexus aurantiacus
NADPH
-
Chloroflexus aurantiacus