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Literature summary for 1.2.1.73 extracted from

  • Krejcik, Z.; Denger, K.; Weinitschke, S.; Hollemeyer, K.; Paces, V.; Cook, A.M.; Smits, T.H.
    Sulfoacetate released during the assimilation of taurine-nitrogen by Neptuniibacter caesariensis: purification of sulfoacetaldehyde dehydrogenase (2008), Arch. Microbiol., 190, 159-168.
    View publication on PubMed
Search for more literature for 1.2.1.73

Cloned(Commentary)

Cloned (Comment) Organism
gene safD, DNA and amino acid sequence determination and analysis, genetic organization Neptuniibacter caesariensis

General Stability

General Stability Organism
SafD is stable in Tris-HCl buffer, pH 9.0 Neptuniibacter caesariensis

Inhibitors

Inhibitors Comment Organism Structure
NADP+ low activity an inhibition at higher concentration Neptuniibacter caesariensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 NAD+ pH 9.0, 22°C Neptuniibacter caesariensis
0.49
-
 Sulfoacetaldehyde pH 9.0, 22°C Neptuniibacter caesariensis
4.5
-
 NADP+ pH 9.0, 22°C Neptuniibacter caesariensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Neptuniibacter caesariensis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Neptuniibacter caesariensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49100
-
 4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation Neptuniibacter caesariensis
50000
-
 4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation Neptuniibacter caesariensis
190000
-
 gel filtration Neptuniibacter caesariensis
196500
-
 sequence calculation Neptuniibacter caesariensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-sulfoacetaldehyde + H2O + NAD+ Neptuniibacter caesariensis
-
 sulfoacetate + NADH + H+
-
 ?
additional information Neptuniibacter caesariensis the organism assimilates taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter, taurine:pyruvate aminotransferase, a sulfoacetaldehyde dehydrogenase, and exporter(s) of sulfoacetate, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Neptuniibacter caesariensis
-
 formerly Oceanospirillum sp., CCUG 52065, a marine gammaproteobacterium, strain MED92, gene safD encoding an inducible enzyme
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 610fold to homogeneity by anion exchange and hydrophobic interaction chromatography Neptuniibacter caesariensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-sulfoacetaldehyde + H2O + NAD(P)+ the enzyme is specific for sulfoacetaldehyde, NAD+ is the highly preferred cofactor to NADP+ Neptuniibacter caesariensis sulfoacetate + NAD(P)H + H+
-
 ?
2-sulfoacetaldehyde + H2O + NAD+
-
 Neptuniibacter caesariensis sulfoacetate + NADH + H+
-
 ?
additional information the organism assimilates taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter, taurine:pyruvate aminotransferase, a sulfoacetaldehyde dehydrogenase, and exporter(s) of sulfoacetate, overview Neptuniibacter caesariensis ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation Neptuniibacter caesariensis

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the NAD-dependent aldehyde dehydrogenase superfamily Neptuniibacter caesariensis
SafD
-
 Neptuniibacter caesariensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Neptuniibacter caesariensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Neptuniibacter caesariensis

pH Range

pH Minimum pH Maximum Comment Organism
7.2 10.5 50% of maximal activity at pH 7.2, 70% at pH 8.0, and 80% at pH 9.5-10.5 Neptuniibacter caesariensis

Cofactor

Cofactor Comment Organism Structure
NAD+ highly preferred cofactor Neptuniibacter caesariensis
NADP+ low activity and inhibition at higher concentration Neptuniibacter caesariensis