BRENDA - Enzyme Database
show all sequences of 1.2.1.73

Sulfoacetate released during the assimilation of taurine-nitrogen by Neptuniibacter caesariensis: purification of sulfoacetaldehyde dehydrogenase

Krejcik, Z.; Denger, K.; Weinitschke, S.; Hollemeyer, K.; Paces, V.; Cook, A.M.; Smits, T.H.; Arch. Microbiol. 190, 159-168 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene safD, DNA and amino acid sequence determination and analysis, genetic organization
Neptuniibacter caesariensis
General Stability
General Stability
Organism
SafD is stable in Tris-HCl buffer, pH 9.0
Neptuniibacter caesariensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
NADP+
low activity an inhibition at higher concentration
Neptuniibacter caesariensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
NAD+
pH 9.0, 22°C
Neptuniibacter caesariensis
0.49
-
Sulfoacetaldehyde
pH 9.0, 22°C
Neptuniibacter caesariensis
4.5
-
NADP+
pH 9.0, 22°C
Neptuniibacter caesariensis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Neptuniibacter caesariensis
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Neptuniibacter caesariensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49100
-
4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation
Neptuniibacter caesariensis
50000
-
4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation
Neptuniibacter caesariensis
190000
-
gel filtration
Neptuniibacter caesariensis
196500
-
sequence calculation
Neptuniibacter caesariensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-sulfoacetaldehyde + H2O + NAD+
Neptuniibacter caesariensis
-
sulfoacetate + NADH + H+
-
-
?
additional information
Neptuniibacter caesariensis
the organism assimilates taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter, taurine:pyruvate aminotransferase, a sulfoacetaldehyde dehydrogenase, and exporter(s) of sulfoacetate, overview
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Neptuniibacter caesariensis
-
formerly Oceanospirillum sp., CCUG 52065, a marine gammaproteobacterium, strain MED92, gene safD encoding an inducible enzyme
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme 610fold to homogeneity by anion exchange and hydrophobic interaction chromatography
Neptuniibacter caesariensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-sulfoacetaldehyde + H2O + NAD(P)+
the enzyme is specific for sulfoacetaldehyde, NAD+ is the highly preferred cofactor to NADP+
684762
Neptuniibacter caesariensis
sulfoacetate + NAD(P)H + H+
-
-
-
?
2-sulfoacetaldehyde + H2O + NAD+
-
684762
Neptuniibacter caesariensis
sulfoacetate + NADH + H+
-
-
-
?
additional information
the organism assimilates taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter, taurine:pyruvate aminotransferase, a sulfoacetaldehyde dehydrogenase, and exporter(s) of sulfoacetate, overview
684762
Neptuniibacter caesariensis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation
Neptuniibacter caesariensis
Synonyms
Synonyms
Commentary
Organism
More
the enzyme belongs to the NAD-dependent aldehyde dehydrogenase superfamily
Neptuniibacter caesariensis
SafD
-
Neptuniibacter caesariensis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Neptuniibacter caesariensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Neptuniibacter caesariensis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.2
10.5
50% of maximal activity at pH 7.2, 70% at pH 8.0, and 80% at pH 9.5-10.5
Neptuniibacter caesariensis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
highly preferred cofactor
Neptuniibacter caesariensis
NADP+
low activity and inhibition at higher concentration
Neptuniibacter caesariensis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene safD, DNA and amino acid sequence determination and analysis, genetic organization
Neptuniibacter caesariensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
highly preferred cofactor
Neptuniibacter caesariensis
NADP+
low activity and inhibition at higher concentration
Neptuniibacter caesariensis
General Stability (protein specific)
General Stability
Organism
SafD is stable in Tris-HCl buffer, pH 9.0
Neptuniibacter caesariensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NADP+
low activity an inhibition at higher concentration
Neptuniibacter caesariensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
NAD+
pH 9.0, 22°C
Neptuniibacter caesariensis
0.49
-
Sulfoacetaldehyde
pH 9.0, 22°C
Neptuniibacter caesariensis
4.5
-
NADP+
pH 9.0, 22°C
Neptuniibacter caesariensis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Neptuniibacter caesariensis
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Neptuniibacter caesariensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49100
-
4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation
Neptuniibacter caesariensis
50000
-
4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation
Neptuniibacter caesariensis
190000
-
gel filtration
Neptuniibacter caesariensis
196500
-
sequence calculation
Neptuniibacter caesariensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-sulfoacetaldehyde + H2O + NAD+
Neptuniibacter caesariensis
-
sulfoacetate + NADH + H+
-
-
?
additional information
Neptuniibacter caesariensis
the organism assimilates taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter, taurine:pyruvate aminotransferase, a sulfoacetaldehyde dehydrogenase, and exporter(s) of sulfoacetate, overview
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 610fold to homogeneity by anion exchange and hydrophobic interaction chromatography
Neptuniibacter caesariensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-sulfoacetaldehyde + H2O + NAD(P)+
the enzyme is specific for sulfoacetaldehyde, NAD+ is the highly preferred cofactor to NADP+
684762
Neptuniibacter caesariensis
sulfoacetate + NAD(P)H + H+
-
-
-
?
2-sulfoacetaldehyde + H2O + NAD+
-
684762
Neptuniibacter caesariensis
sulfoacetate + NADH + H+
-
-
-
?
additional information
the organism assimilates taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter, taurine:pyruvate aminotransferase, a sulfoacetaldehyde dehydrogenase, and exporter(s) of sulfoacetate, overview
684762
Neptuniibacter caesariensis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation
Neptuniibacter caesariensis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Neptuniibacter caesariensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Neptuniibacter caesariensis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.2
10.5
50% of maximal activity at pH 7.2, 70% at pH 8.0, and 80% at pH 9.5-10.5
Neptuniibacter caesariensis
Other publictions for EC 1.2.1.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
684762
Krejcik
Sulfoacetate released during t ...
Neptuniibacter caesariensis
Arch. Microbiol.
190
159-168
2008
-
-
1
-
-
1
1
3
1
1
4
2
-
4
-
-
1
-
-
-
-
-
3
1
2
1
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
1
-
1
-
3
1
1
4
2
-
-
-
1
-
-
-
-
3
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
675824
Weinitschke
Sulfoacetaldehyde is excreted ...
Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA009
Microbiology
151
1285-1290
2005
-
1
-
-
-
-
-
3
-
1
-
2
-
12
-
-
-
-
-
1
1
-
4
-
1
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
3
-
1
-
2
-
-
-
-
-
1
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684747
Denger
Sulfoacetate generated by Rhod ...
Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA009
Arch. Microbiol.
182
254-258
2004
-
-
-
-
-
-
-
-
-
1
-
2
-
9
-
-
-
-
-
-
-
-
4
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-