Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Methanopyrus kandleri |
Protein Variants | Comment | Organism |
---|---|---|
C393S | 95% of the GluTR reductase activity compared to wild-type enzyme, 100% of the GluTR esterase activity compared to wild-type enzyme | Methanopyrus kandleri |
C42S | no GluTR reductase and GluTR esterase activity | Methanopyrus kandleri |
C48S | 90% of the GluTR reductase activity compared to wild-type enzyme, 95% of the GluTR esterase activity compared to wild-type enzyme | Methanopyrus kandleri |
C6S | 130% of the GluTR reductase activity compared to wild-type enzyme, 120% of the GluTR esterase activity compared to wild-type enzyme | Methanopyrus kandleri |
C90S | 85% of the GluTR reductase activity compared to wild-type enzyme, 105% of the GluTR esterase activity compared to wild-type enzyme | Methanopyrus kandleri |
H84A | no GluTR reductase activity, 5% of the GluTR esterase activity compared to wild-type enzyme | Methanopyrus kandleri |
H84N | 30% of the GluTR reductase activity compared to wild-type enzyme, 15% of the GluTR esterase activity compared to wild-type enzyme | Methanopyrus kandleri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 1.0 mM, 90% inhibition | Methanopyrus kandleri | |
glutamate-1-semialdehyde | 1.0 mM, 50% inhibition | Methanopyrus kandleri | |
glutamycin | 2.5 mM, 75% inhibition | Methanopyrus kandleri | |
heme | 0.007 mM, 70% inhibition | Methanopyrus kandleri | |
iodoacetamide | 0.01 mM, 30% inhibition, 0.1 mM, complete inhibition | Methanopyrus kandleri | |
N-tosyl-L-phenylalaninechloromethyl ketone | 0.1 mM, 90% inhibition, 1.0 mM, complete inhibition | Methanopyrus kandleri | |
PbCl2 | 0.1 mM, 60% inhibition, 1.0 mM, complete inhibition | Methanopyrus kandleri | |
PtCl4 | 0.1 mM, 55% inhibition, 1.0 mM, 90% inhibition | Methanopyrus kandleri | |
ZnCl2 | 0.2 mM, 45% inhibition, 5.0 mM, 90% inhibition | Methanopyrus kandleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45436 | - |
x * 45436, electrospray ionization mass spectrometry | Methanopyrus kandleri |
190000 | - |
gel filtration | Methanopyrus kandleri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | Q9UXR8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamyl-tRNAGlu + NADPH + H+ | in absence of NADPH, an esterase activity of GluTR hydrolyzes the highly reactive thioester of tRNAGlu to release glutamate | Methanopyrus kandleri | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45436, electrospray ionization mass spectrometry | Methanopyrus kandleri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Methanopyrus kandleri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
- |
Methanopyrus kandleri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not possess a chromophoric prosthetic group | Methanopyrus kandleri | |
NADPH | - |
Methanopyrus kandleri |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methanopyrus kandleri | isoelectric focusing | - |
6 |