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Literature summary for 1.2.1.7 extracted from

  • Gosling, A.; Zachariou, M.; Straffon, M.
    Purification and characterisation of a 4-hydroxy benzaldehyde dehydrogenase cloned from Acinetobacter baylyi (2008), Enzyme Microb. Technol., 43, 417-422.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
K+ 125 mM KCl, 1.5 fold increase in activity Acinetobacter baylyi
Na+ 125 mM NaCl, 1.5 fold increase in activity Acinetobacter baylyi

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET-Duet1 for expression in Escherichia coli BL21DE3 cells Acinetobacter baylyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
4-hydroxybenzaldehyde apparent KM Acinetobacter baylyi
0.029
-
benzaldehyde apparent KM Acinetobacter baylyi
0.037
-
3,4-Dihydroxybenzaldehyde apparent KM Acinetobacter baylyi
0.044
-
4-hydroxy-3-methoxybenzaldehyde apparent KM Acinetobacter baylyi
0.6
-
NAD+ apparent KM Acinetobacter baylyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
determined by SDS-PAGE Acinetobacter baylyi
116000
-
determined by gel filtration Acinetobacter baylyi

Organism

Organism UniProt Comment Textmining
Acinetobacter baylyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on a His-Trap FF IMAC column Acinetobacter baylyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
3.2
-
clarified lysate, total activity 37 U, protein concentration 5.77 mg/ml Acinetobacter baylyi
22
-
IMAC eluted 4-hydroxy benzaldehyde dehydrogenase, total activity 35 Units, protein concentration 0.27 mg/ml Acinetobacter baylyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dihydroxybenzaldehyde + NAD+ + H2O
-
Acinetobacter baylyi 2,4-dihydroxybenzoate + NADH + H+
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
Acinetobacter baylyi 3,4-dihydroxybenzoate + NADH + H+
-
?
3-hydroxy-4-methoxybenzaldehyde + NAD+ + H2O
-
Acinetobacter baylyi 3-hydroxy-4-methoxybenzoate + NADH + H+
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
-
Acinetobacter baylyi 3-hydroxybenzoate + NADH + H+
-
?
3-methoxybenzaldehyde + NAD+ + H2O
-
Acinetobacter baylyi 3-methoxybenzoate + NADH + H+
-
?
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
-
Acinetobacter baylyi 4-hydroxy-3-methoxybenzoate + NADH + H+
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
-
Acinetobacter baylyi 4-hydroxybenzoate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O
-
Acinetobacter baylyi benzoate + NADH + H+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 55000 Acinetobacter baylyi

Synonyms

Synonyms Comment Organism
4-hydroxy benzaldehyde dehydrogenase
-
Acinetobacter baylyi

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
21
-
activity assay Acinetobacter baylyi

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
21 45 21┬░C 100%, 37┬░C 90%, 45┬░C 75% activity Acinetobacter baylyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
4-hydroxybenzaldehyde apparent Kcat Acinetobacter baylyi
4.6
-
benzaldehyde apparent Kcat Acinetobacter baylyi
14
-
NAD+ apparent Kcat Acinetobacter baylyi
18
-
4-hydroxy-3-methoxybenzaldehyde apparent Kcat Acinetobacter baylyi
25
-
3,4-Dihydroxybenzaldehyde apparent Kcat Acinetobacter baylyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
Acinetobacter baylyi

pH Range

pH Minimum pH Maximum Comment Organism
6.9 10.7 the activities measured at pH 6.85 and 10.7 are approximately 30% of the activity measured at pH 9.3 Acinetobacter baylyi

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Acinetobacter baylyi

pI Value

Organism Comment pI Value Maximum pI Value
Acinetobacter baylyi determined by isoelectric focusing electrophoresis
-
5.6
Acinetobacter baylyi predicted from the amino acid sequence
-
5.8