Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | or K+ or Rb+ required for maximal activity | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Pseudomonas fluorescens | |
p-hydroxymercuribenzoate | - |
Pseudomonas fluorescens | |
Zn2+ | - |
Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
benzaldehyde | - |
Pseudomonas fluorescens | |
0.037 | - |
NADP+ | - |
Pseudomonas fluorescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | or NH4+ or Rb+ required for maximal activity | Pseudomonas fluorescens | |
Rb+ | or K+ or NH4+ required for maximal activity | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
sucrose density gradient centrifugation | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzaldehyde + NADP+ + H2O | Pseudomonas fluorescens | - |
4-hydroxybenzoate + NADPH + H+ | - |
? | |
benzaldehyde + NADP+ + H2O | Pseudomonas fluorescens | - |
benzoate + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.14 | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-17°C, pH 7.2, 10 mM dithiothreitol, several months | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzaldehyde + NADP+ + H2O | - |
Pseudomonas fluorescens | 4-hydroxybenzoate + NADPH + H+ | - |
? | |
benzaldehyde + NADP+ + H2O | - |
Pseudomonas fluorescens | benzoate + NADPH | - |
? | |
isovanillin + NADP+ + H2O | - |
Pseudomonas fluorescens | 3-hydroxy-4-methoxybenzoate + NADPH | - |
? | |
salicylaldehyde + NADP+ + H2O | - |
Pseudomonas fluorescens | salicylic acid + NADPH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
heat-labile | Pseudomonas fluorescens |
50 | - |
inactivation after 5 min | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Pseudomonas fluorescens |