Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | or K+ or Rb+ required for maximal activity | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | or NH4+ or Rb+ required for maximal activity | Pseudomonas putida | |
Rb+ | or K+ or NH4+ required for maximal activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56300 | - |
4 * 56300, SDS-PAGE | Pseudomonas putida |
122000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NADP+ + H2O | Pseudomonas putida | Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 | benzoate + NADPH | - |
ir | |
benzaldehyde + NADP+ + H2O | Pseudomonas putida MT53 | Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 | benzoate + NADPH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
MT53 | - |
Pseudomonas putida MT53 | - |
MT53 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60.2 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NADP+ + H2O | Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 | Pseudomonas putida | benzoate + NADPH | - |
ir | |
benzaldehyde + NADP+ + H2O | Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 | Pseudomonas putida MT53 | benzoate + NADPH | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 56300, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | enzyme on Pseudomonas plasmid | Pseudomonas putida | |
NADP+ | - |
Pseudomonas putida |