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Literature summary for 1.2.1.7 extracted from

  • Chalmers, R.M.; Scott, A.J.; Fewson, C.A.
    Purification of the benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by the TOL plasmid pWW53 of Pseudomonas putida MT53 and their preliminary comparison with benzyl alcohol dehydrogenase and benzaldehyde dehydrogenases I and II from Acinetobacter calcoaceticus (1990), J. Gen. Microbiol., 136, 637-643.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
NH4+ or K+ or Rb+ required for maximal activity Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
K+ or NH4+ or Rb+ required for maximal activity Pseudomonas putida
Rb+ or K+ or NH4+ required for maximal activity Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56300
-
4 * 56300, SDS-PAGE Pseudomonas putida
122000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzaldehyde + NADP+ + H2O Pseudomonas putida Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 benzoate + NADPH
-
ir
benzaldehyde + NADP+ + H2O Pseudomonas putida MT53 Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 benzoate + NADPH
-
ir

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
MT53
-
Pseudomonas putida MT53
-
MT53
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
60.2
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + NADP+ + H2O Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 Pseudomonas putida benzoate + NADPH
-
ir
benzaldehyde + NADP+ + H2O Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 Pseudomonas putida MT53 benzoate + NADPH
-
ir

Subunits

Subunits Comment Organism
tetramer 4 * 56300, SDS-PAGE Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ enzyme on Pseudomonas plasmid Pseudomonas putida
NADP+
-
Pseudomonas putida