BRENDA - Enzyme Database show
show all sequences of 1.2.1.7

Anaerobic degradation of toluene in denitrifying Pseudomonas sp.: indication for toluene methylhydroxylation and benzoyl-CoA as central aromatic intermediate

Altenschmidt, U.; Fuchs, G.; Arch. Microbiol. 156, 152-158 (1991)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
benzaldehyde
-
Pseudomonas sp.
0.13
-
NADP+
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzaldehyde + NADP+ + H2O
Pseudomonas sp.
-
benzoate + NADPH
-
Pseudomonas sp.
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzaldehyde + NADP+ + H2O
-
288326
Pseudomonas sp.
benzoate + NADPH
-
288326
Pseudomonas sp.
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
benzaldehyde
-
Pseudomonas sp.
0.13
-
NADP+
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzaldehyde + NADP+ + H2O
Pseudomonas sp.
-
benzoate + NADPH
-
Pseudomonas sp.
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzaldehyde + NADP+ + H2O
-
288326
Pseudomonas sp.
benzoate + NADPH
-
288326
Pseudomonas sp.
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Pseudomonas sp.
Other publictions for EC 1.2.1.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697657
Gosling
-
Purification and characterisat ...
Acinetobacter baylyi
Enzyme Microb. Technol.
43
417-422
2008
2
-
1
-
-
-
-
5
-
-
2
-
-
1
-
-
1
-
-
-
2
-
8
1
1
1
-
5
1
1
-
1
-
2
-
2
-
1
1
-
-
-
-
-
-
5
-
-
2
-
-
-
-
1
-
-
2
-
8
1
1
1
-
5
1
1
-
2
-
-
-
-
-
-
288335
Kohler
-
Localization of a tumor-associ ...
Platichthys flesus, Rattus norvegicus
Mar. Environ. Res.
46
185-189
1998
-
-
-
-
-
-
2
-
-
-
-
2
-
2
-
-
-
2
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288336
Biergert
-
Anaerobic oxidation of toluene ...
Thauera sp.
Arch. Microbiol.
163
407-417
1995
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288326
Altenschmidt
Anaerobic degradation of tolue ...
Pseudomonas sp.
Arch. Microbiol.
156
152-158
1991
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4814
Tsou
Mandelate pathway of Pseudomon ...
Pseudomonas putida
Biochemistry
29
9856-9862
1990
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288328
Chalmers
-
Purification of the benzyl alc ...
Pseudomonas putida, Pseudomonas putida MT53
J. Gen. Microbiol.
136
637-643
1990
1
-
-
-
-
-
-
-
-
2
2
2
-
2
-
-
1
-
-
-
1
-
2
1
-
-
-
-
1
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
2
2
-
-
-
1
-
-
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288329
Harayama
Characterization of five genes ...
Pseudomonas putida
J. Bacteriol.
171
5048-5055
1989
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288330
Shaw
Physical and functional mappin ...
Pseudomonas putida
J. Gen. Microbiol.
134
2463-2474
1988
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288331
Keil
Gene organization of the first ...
Pseudomonas putida
J. Bacteriol.
169
764-770
1987
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4801
Van den Tweel
-
Microbial metabolism of D- and ...
Pseudomonas putida
Arch. Microbiol.
144
169-174
1986
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288333
Bhat
Involvement of 4-hydroxymandel ...
Pseudomonas putida
J. Bacteriol.
127
1108-1118
1976
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
1
-
-
1
-
3
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
1
-
3
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
288334
Stachow
Purification and properties of ...
Pseudomonas fluorescens
J. Biol. Chem.
242
5294-5300
1967
1
-
-
-
-
-
3
2
-
2
1
2
-
1
-
-
1
-
-
-
1
1
4
-
-
-
2
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
3
-
2
-
2
1
2
-
-
-
1
-
-
1
1
4
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-