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Literature summary for 1.2.1.7 extracted from

  • Stachow, C.S.; Stevenson, I.L.; Day, D.
    Purification and properties of nicotinamide adenine dinucleotide phosphate-specific benzaldehyde dehydrogenase from Pseudomonas (1967), J. Biol. Chem., 242, 5294-5300.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NH4+ or K+ or Rb+ required for maximal activity Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide
-
Pseudomonas fluorescens
p-hydroxymercuribenzoate
-
Pseudomonas fluorescens
Zn2+
-
Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
benzaldehyde
-
Pseudomonas fluorescens
0.037
-
NADP+
-
Pseudomonas fluorescens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ or NH4+ or Rb+ required for maximal activity Pseudomonas fluorescens
Rb+ or K+ or NH4+ required for maximal activity Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
sucrose density gradient centrifugation Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybenzaldehyde + NADP+ + H2O Pseudomonas fluorescens
-
4-hydroxybenzoate + NADPH + H+
-
?
benzaldehyde + NADP+ + H2O Pseudomonas fluorescens
-
benzoate + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
2.14
-
-
Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
-17┬░C, pH 7.2, 10 mM dithiothreitol, several months Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzaldehyde + NADP+ + H2O
-
Pseudomonas fluorescens 4-hydroxybenzoate + NADPH + H+
-
?
benzaldehyde + NADP+ + H2O
-
Pseudomonas fluorescens benzoate + NADPH
-
?
isovanillin + NADP+ + H2O
-
Pseudomonas fluorescens 3-hydroxy-4-methoxybenzoate + NADPH
-
?
salicylaldehyde + NADP+ + H2O
-
Pseudomonas fluorescens salicylic acid + NADPH
-
?

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
additional information
-
heat-labile Pseudomonas fluorescens
50
-
inactivation after 5 min Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Pseudomonas fluorescens