BRENDA - Enzyme Database
show all sequences of 1.2.1.68

Highly efficient biotransformation of eugenol to ferulic acid and further conversion to vanillin in recombinant strains of Escherichia coli

Overhage, J.; Steinbuchel, A.; Priefert, H.; Appl. Environ. Microbiol. 69, 6569-6576 (2003)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
highly efficient two-step biotransformation of eugenol to ferulic acid and further conversion to vanillin in recombinant strains of Escherichia coli. Maximum production rate for ferulic acid at large scale is 14.4 mM per h per liter of culture, yield of 93.3% with respect to the added amount of eugenol
Pseudomonas sp.
Engineering
Protein Variants
Commentary
Organism
additional information
expression of the vaoA gene from Penicillium simplicissimum CBS 170.90, encoding vanillyl alcohol oxidase, which also catalyzes the conversion of eugenol to coniferyl alcohol, is expressed in Escherichia coli XL-1 Blue under the control of the lac promoter, together with the genes calA and calB, encoding coniferyl alcohol dehydrogenase and coniferyl aldehyde dehydrogenase of Pseudomonas sp. strain HR199, respectively
Pseudomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
O86447
-
-
Application (protein specific)
Application
Commentary
Organism
biotechnology
highly efficient two-step biotransformation of eugenol to ferulic acid and further conversion to vanillin in recombinant strains of Escherichia coli. Maximum production rate for ferulic acid at large scale is 14.4 mM per h per liter of culture, yield of 93.3% with respect to the added amount of eugenol
Pseudomonas sp.
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
expression of the vaoA gene from Penicillium simplicissimum CBS 170.90, encoding vanillyl alcohol oxidase, which also catalyzes the conversion of eugenol to coniferyl alcohol, is expressed in Escherichia coli XL-1 Blue under the control of the lac promoter, together with the genes calA and calB, encoding coniferyl alcohol dehydrogenase and coniferyl aldehyde dehydrogenase of Pseudomonas sp. strain HR199, respectively
Pseudomonas sp.
Other publictions for EC 1.2.1.68
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742102
Adeboye
A coniferyl aldehyde dehydrog ...
Pseudomonas sp. HR199, Pseudomonas sp. HR199 DSM 7063
Biores. Technol.
212
11-19
2016
-
-
1
-
1
-
-
-
-
-
-
2
-
6
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654275
Overhage
Highly efficient biotransforma ...
Pseudomonas sp.
Appl. Environ. Microbiol.
69
6569-6576
2003
-
1
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654264
Overhage
Biotransformation of eugenol t ...
Pseudomonas sp.
Appl. Environ. Microbiol.
68
4315-4321
2002
-
1
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288325
Achterholt
Purification and characterizat ...
Pseudomonas sp.
J. Bacteriol.
180
4387-4391
1998
-
1
-
-
-
-
-
2
-
-
2
1
-
2
-
-
1
-
-
1
1
-
2
1
1
1
-
1
-
1
-
1
1
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
1
1
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-