BRENDA - Reference to 1.2.1.68; Id = 654264

biotechnology

biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16. The gene calB, encoding coniferyl aldehyde dehydrogenase, and ehyAB and calA encoding eugenol hydroxylase and coniferyl alcohol dehydrogenase, respectively, are amplified and combined to construct a catabolic gene cassette. This gene cassette is cloned in the broad-host-range vector pBBR1-JO2 and transferred to Ralstonia eutropha H16. A recombinant strain of Ralstonia eutropha H16 harboring this plasmid expresses functionally active eugenol hydroxylase, coniferyl alcohol dehydrogenase, and coniferyl aldehyde dehydrogenase. Cells of Ralstonia eutropha H16 from the late-exponential growth phase are used asa biocatalysts for the biotransformation of eugenol to ferulic acid. A maximum conversion rate of 2.9 mM of eugenol per h per liter of culture is achieved with a yield of 93.8 mol% of ferulic acid from eugenol within 20 h, without further optimization

Pseudomonas sp.

coniferyl aldehyde + H2O + NAD+

Pseudomonas sp.

involved in eugenol catabolism

ferulate + NADH

-

?

Pseudomonas sp.

Q86447

-

coniferyl aldehyde + H2O + NAD+

-

654264

Pseudomonas sp.

ferulate + NADH

-

?

coniferyl aldehyde + H2O + NAD+

involved in eugenol catabolism

654264

Pseudomonas sp.

ferulate + NADH

-

?

coniferyl aldehyde dehydrogenase

-

Pseudomonas sp.

biotechnology

biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16. The gene calB, encoding coniferyl aldehyde dehydrogenase, and ehyAB and calA encoding eugenol hydroxylase and coniferyl alcohol dehydrogenase, respectively, are amplified and combined to construct a catabolic gene cassette. This gene cassette is cloned in the broad-host-range vector pBBR1-JO2 and transferred to Ralstonia eutropha H16. A recombinant strain of Ralstonia eutropha H16 harboring this plasmid expresses functionally active eugenol hydroxylase, coniferyl alcohol dehydrogenase, and coniferyl aldehyde dehydrogenase. Cells of Ralstonia eutropha H16 from the late-exponential growth phase are used asa biocatalysts for the biotransformation of eugenol to ferulic acid. A maximum conversion rate of 2.9 mM of eugenol per h per liter of culture is achieved with a yield of 93.8 mol% of ferulic acid from eugenol within 20 h, without further optimization

Pseudomonas sp.

coniferyl aldehyde + H2O + NAD+

Pseudomonas sp.

involved in eugenol catabolism

ferulate + NADH

-

?

coniferyl aldehyde + H2O + NAD+

-

654264

Pseudomonas sp.

ferulate + NADH

-

?

coniferyl aldehyde + H2O + NAD+

involved in eugenol catabolism

654264

Pseudomonas sp.

ferulate + NADH

-

?

742102

Adeboye

A coniferyl aldehyde dehydrog ...

Pseudomonas sp. HR199, Pseudomonas sp. HR199 DSM 7063

Biores. Technol.

212

11-19

2016

-

1

-

1

-

2

-

6

-

1

-

2

-

1

-

1

-

1

-

1

-

2

-

1

-

2

-

654275

Overhage

Highly efficient biotransforma ...

Pseudomonas sp.

Appl. Environ. Microbiol.

69

6569-6576

2003

-

1

-

1

-

6

-

1

-

1

-

654264

Overhage

Biotransformation of eugenol t ...

Pseudomonas sp.

Appl. Environ. Microbiol.

68

4315-4321

2002

-

1

-

1

-

3

-

2

-

1

-

1

-

1

-

2

-

288325

Achterholt

Purification and characterizat ...

Pseudomonas sp.

J. Bacteriol.

180

4387-4391

1998

-

1

-

2

-

2

1

-

2

-

1

-

1

-

2

1

-

1

-

1

-

1

-

1

-

1

-

2

-

2

1

-

1

-

1

-

2

1

-

1

-

1

-

1

-

Biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16

Data extracted from this reference: